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Keywords: superoxide
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Biochem J (2018) 475 (21): 3451–3470.
Published: 09 November 2018
... at pH 4.7 with peroxide (H 2 O 2 ), ‘superoxide’ (a ∼50 : 50 mixture of and ), hydroxyl radicals ( • OH, formed in Fenton mixtures), and illuminated riboflavin (generating singlet oxygen, 1 O 2 ). Products were monitored electrophoretically. DHA quenched H 2 O 2 far more effectively than superoxide...
Includes: Supplementary data
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Biochem J (2013) 456 (1): 139–146.
Published: 24 October 2013
... clusters to transfer electrons from the flavin to quinone and an eighth cluster (N1a) on the opposite side of the flavin. The role of cluster N1a is unknown, but Escherichia coli complex I has an unusually high-potential cluster N1a and its reduced flavin produces H 2 O 2 , not superoxide, suggesting...
Includes: Supplementary data
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Biochem J (2011) 437 (3): 381–387.
Published: 13 July 2011
...Markus Schwarzländer; David C. Logan; Mark D. Fricker; Lee J. Sweetlove The properties of a cpYFP [circularly permuted YFP (yellow fluorescent protein)] reported to act as a superoxide sensor have been re-examined in Arabidopsis mitochondria. We have found that the probe has high pH sensitivity...
Includes: Multimedia, Supplementary data
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Biochem J (2011) 436 (2): 493–505.
Published: 13 May 2011
...Philippe Pasdois; Joanne E. Parker; Elinor J. Griffiths; Andrew P. Halestrap Oxidized cytochrome c is a powerful superoxide scavenger within the mitochondrial IMS (intermembrane space), but the importance of this role in situ has not been well explored. In the present study, we investigated...
Includes: Supplementary data
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Biochem J (2009) 422 (2): 373–382.
Published: 13 August 2009
...Kei Miyano; Hirofumi Koga; Reiko Minakami; Hideki Sumimoto Rac1 and Rac2, which belong to the Rho subfamily of Ras-related GTPases, play an essential role in activation of gp91 phox /Nox2 (cytochrome b -245, β polypeptide; also known as Cybb), the catalytic core of the superoxide-producing NADPH...
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Biochem J (2009) 421 (1): 79–86.
Published: 12 June 2009
... of inflammatory disease. They are believed to exert protective effects principally by acting as superoxide dismutase mimetics or radical scavengers. However, we show here that nitroxides can also potently inhibit MPO-mediated HOCl production, with the nitroxide 4-aminoTEMPO inhibiting HOCl production by MPO...
Includes: Supplementary data
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Biochem J (2009) 417 (3): 773–781.
Published: 16 January 2009
.... Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose...
Includes: Supplementary data
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Biochem J (2009) 417 (1): 1–13.
Published: 12 December 2008
...Michael P. Murphy The production of ROS (reactive oxygen species) by mammalian mitochondria is important because it underlies oxidative damage in many pathologies and contributes to retrograde redox signalling from the organelle to the cytosol and nucleus. Superoxide (O 2 •− ) is the proximal...
Includes: Multimedia, Supplementary data
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Biochem J (2008) 415 (1): 57–65.
Published: 12 September 2008
... and Rac1. NADPH-specific superoxide generating activity was reduced by 80% in the presence of either a flavoenzyme inhibitor DPI (diphenyleneiodonium) or NADP + . The plasma membranes from PMA-stimulated cells showed an increased amount of Rac1 (19.6 pmol/mg), as compared with the membranes from...
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Biochem J (2007) 406 (1): 105–114.
Published: 26 July 2007
... NOX4-expressing cells was H 2 O 2 , whereas superoxide (O 2 − ) was almost undetectable. Probes that allow detection of intracellular O 2 − generation yielded differential results: DHE (dihydroethidium) fluorescence and ACP (1-acetoxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine) ESR measurements did...
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Biochem J (2004) 381 (1): 241–248.
Published: 22 June 2004
...Christine C. WINTERBOURN; Helena N. PARSONS-MAIR; Silvia GEBICKI; Janusz M. GEBICKI; Michael J. DAVIES Superoxide reacts rapidly with other radicals, but these reactions have received little attention in the context of oxidative stress. For tyrosyl radicals, reaction with superoxide is 3-fold...
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Biochem J (2002) 368 (2): 545–553.
Published: 01 December 2002
... superoxide Abbreviations used: FCCP, p -trifluoromethoxyphenylhydrazone carbonyl cyanide; β-HOB, d -β-hydroxybutyrate; ROS, reactive oxygen species; ∗ROS site, site of superoxide generation. Biochem. J. (2002) 368, 545 553 (Printed in Great Britain) 545 Complex I-mediated reactive oxygen...
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Biochem J (2002) 362 (2): 253–258.
Published: 22 February 2002
... Society, London ©2002 2002 1 To whom correspondence should be addressed (e-mail [email protected] ). 11 9 2001 12 11 2001 12 12 2001 copper manganese neurodegeneration scrapie superoxide Abbreviations used: BCA, bicinchoninic acid; NBT, Nitro Blue Tetrazolium...
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Biochem J (2000) 349 (1): 113–117.
Published: 26 June 2000
... 2000 host defence oxygen radicals p47 phox respiratory burst superoxide Biochem. J. (2000) 349, 113 117 (Printed in Great Britain) 113 p40phox participates in the activation of NADPH oxidase by increasing the affinity of p47phox for flavocytochrome b558 Andrew R. CROSS Department...
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Biochem J (2000) 349 (1): 369–375.
Published: 26 June 2000
...-actin magnesium ion phagocytosis superoxide Biochem. J. (2000) 349, 369 375 (Printed in Great Britain) 369 Deactivation of neutrophil NADPH oxidase by actin-depolymerizing agents in a cell-free system Minoru TAMURA1, Masamichi KANNO and Yaeta ENDO Department of Applied Biochemistry, Faculty...
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Biochem J (2000) 345 (2): 195–200.
Published: 10 January 2000
.... The effect of activation was sensitive to the antioxidant enzymes superoxide dismutase and catalase. When ascorbate was oxidized in situ by chemical or enzymic oxidation, the rates of uptake were similar to those after activation of the cells by phorbol ester; however, in the latter case the extracellular...
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