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Keyword: surface plasmon resonance
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Articles
Biochem J (2017) 474 (21): 3675-3687.
Published: 23 October 2017
... provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites. Using surface plasmon resonance, a tandem P1BS sequence showed ∼50-fold higher affinity for MBPAtdPHR1 (a fusion protein comprising the DNA-binding domain and coiled-coil domain of AtPHR1 fused to maltose-binding protein...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (4): 539-556.
Published: 03 February 2017
... positively charged surface presented by the β1–β2 loop and that it binds with somewhat higher affinity to PtdIns(3,4,5)P 3 compared with PtdIns(4,5)P 2 . Surface plasmon resonance (SPR) with lipid headgroups immobilised and the PH domain as an analyte indicate affinities of 300 µM for PtdIns(3,4,5)P 3 and 1...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (1): 79-104.
Published: 22 December 2016
.... Specifically, the carboxy terminus of dopamine D1A was found to directly bind t-SNARE-associated protein snapin in teleost and mammalian hair cell models by yeast two-hybrid (Y2H) and pull-down assays, and snapin directly interacts with hair cell calcium-sensor otoferlin. Surface plasmon resonance (SPR...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (17): 2645-2654.
Published: 30 August 2016
... dissociation rate k d resulting in an overall 12-fold decreased binding affinity in surface plasmon resonance (SPR) experiments. The delayed dissociation might provide BoNT/A more time for endocytosis into synaptic vesicles. In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding a...
Includes: Supplementary data
Articles
Biochem J (2015) 465 (3): 443-457.
Published: 22 January 2015
.... Fourier transform IR and surface plasmon resonance studies also indicated different modes of interaction for LL-37 and RL-37 with anionic and neutral membranes. This correlated with a distinctly different mode of bacterial membrane permeabilization, as determined using a flow cytometric method involving...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (1): 137-149.
Published: 10 December 2013
... extracellular matrix, which could provide new insights into its biological roles. We identified 17 new partners of PCPE-1 by SPR (surface plasmon resonance) imaging. PCPE-1 forms a transient complex with the β-amyloid peptide, whereas it forms high or very high affinity complexes with laminin-111 ( K D =58.8 pM...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (3): 417-428.
Published: 29 May 2012
... glycosaminoglycan composed of sulfated S-domains and unsulfated NA-domains. To elucidate the molecular mechanism of binding of FH to glycosaminoglycans, we performed ultracentrifugation, X-ray scattering and surface plasmon resonance with FH and glycosaminoglycan fragments. Ultracentrifugation showed that FH formed...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (1): 237-245.
Published: 14 December 2011
...-terminal deletion mutants of 4E-BPn ( n =1–3) subtypes were investigated by SPR (surface plasmon resonance) analysis and ITC (isothermal titration calorimetry). Consequently, the conserved PGVTS/T motif within the C-terminal region was shown to act as the second binding region and to play an important role...
Articles
Biochem J (2011) 438 (2): 291-301.
Published: 12 August 2011
... identified in the protein, thus making it a potential new member of the BH3-only protein family. In the present study, we provide NMR, SPR (surface plasmon resonance) and crystallographic evidence that a peptide spanning residues 147–172 in SOUL interacts with the anti-apoptotic protein Bcl-xL. We have...
Includes: Supplementary data
Articles
Biochem J (2009) 419 (3): 577-584.
Published: 14 April 2009
... against PCSK9. Studies using surface plasmon resonance demonstrated that direct binding of PCSK9 to the LDLR could be abolished with three different anti-PCSK9 antibodies. Two of these antibodies were raised against peptide epitopes in a region of the catalytic domain of PCSK9 that is involved in the...
Articles
Biochem J (2009) 419 (1): 221-230.
Published: 13 March 2009
... quantitative DNA-binding study of this plant-specific family of proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics...
Includes: Supplementary data
Articles
Biochem J (2006) 398 (3): 381-392.
Published: 29 August 2006
... fruit-body lectins surface plasmon resonance lipid monolayer The protein world is classically divided into soluble and membrane proteins, the latter comprising approx. 30% of all proteins. There is, however, another large group that spans both definitions. Peripheral or amphitrophic proteins...
Articles
Biochem J (2005) 391 (3): 659-666.
Published: 25 October 2005
... rapid and sensitive in vitro assays. Here, we present new automatized bioassays to detect VAMP cleavage by BoNT/B and F. Western blotting and SPR (surface plasmon resonance) methods revealed that BoNT/B and F totally cleave their substrate on immunoisolated SVs (synaptic vesicles). Real-time monitoring...
Articles
Biochem J (2005) 390 (1): 177-188.
Published: 09 August 2005
... surface plasmon resonance studies with model phosphatidylcholine/cholesterol membranes. The mode of interaction changes radically for a peptide with a stable, preformed helical conformation compared with others that form a structure only on membrane binding. The close correlation between effects observed...
Includes: Supplementary data
Articles
Biochem J (2005) 387 (3): 639-647.
Published: 26 April 2005
... Sic1 surface plasmon resonance Cdks (cyclin-dependent kinases) play an essential regulatory role in cell cycle progression: it is in fact the sequential activation of Cdks by specific, unstable, regulatory subunits, named cyclins, that first triggers the onset of DNA replication and later...
Articles
Biochem J (2004) 381 (3): 725-733.
Published: 27 July 2004
...Mili KAPOOR; P. L. Swarna MUKHI; Namita SUROLIA; K. SUGUNA; Avadhesha SUROLIA The binding of enoyl-ACP (acyl-carrier protein) reductase from Plasmodium falciparum (PfENR) with its substrates and inhibitors has been analysed by SPR (surface plasmon resonance). The binding of the substrate analogue...
Includes: Supplementary data
Articles
Biochem J (2004) 378 (3): 801-808.
Published: 15 March 2004
..., regulator of G-protein signalling; SPR, surface plasmon resonance; TEV, tobacco etch virus. Biochem. J. (2004) 378, 801 808 (Printed in Great Britain) 801 Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an...
Articles
Biochem J (2003) 375 (3): 623-631.
Published: 01 November 2003
... techniques. Surface plasmon resonance analysis indicated a high affinity in the interaction between eIF2β and CK2α, whereas the affinity for the association with CK2β is much lower. Free CK2α is unable to phosphorylate eIF2β, whereas up to 1.2 mol of phosphate/mol of eIF2β was incorporated by the...
Articles
Biochem J (2003) 373 (1): 115-123.
Published: 01 July 2003
...-mediated inhibition of cytochrome c reduction by flavocytochrome b 2 , competitive ELISA tests and surface plasmon resonance. We have thus defined a minimal epitope surface on the haem domain; it encompasses positions 63, 64, 65, 67, 69 and 70 and one or both haem propionates. When the haem and flavin...
Articles
Biochem J (2003) 372 (1): 271-278.
Published: 15 May 2003
... kinase domains was facilitated by PKA-mediated phosphorylation of the kinase domain, as evaluated by surface plasmon resonance. This suggests that an overall structural domain organization and interaction between the kinase and SH3 domains are important for the activity of Csk and its regulation by PKA...
Articles
Biochem J (2002) 368 (1): 111-119.
Published: 15 November 2002
... to homogeneous glycoclusters. The thiol group of the cysteine residue was used to tag the glycosylated oligolysines upon reaction with fluorescein iodoacetamide. The affinity of these glycoclusters towards two plant lectins was assessed by surface plasmon resonance. The selectivity of their cell...
Articles
Biochem J (2002) 366 (2): 491-500.
Published: 01 September 2002
... bacterial protein expression glycosaminoglycan mitogen nuclear localization surface plasmon resonance Abbreviations used: FCS, foetal calf serum; FGF, fibroblast growth factor; GAG, glycosaminoglycan; GFP, green fluorescent protein; HDGF, hepatoma-derived growth factor; hath, homologous with...
Articles
Biochem J (2002) 364 (2): 485-495.
Published: 01 June 2002
... ). 12 10 2001 4 2 2002 13 3 2002 The Biochemical Society, London ©2002 2002 glycoprotein structure glycosylation hCG surface plasmon resonance Abbreviations used: 2D, two-dimensional; hCG, human chorionic gonadotropin; αhCG[glycan 52,78 ], native-hCG-derived α-subunit...
Articles
Biochem J (2002) 364 (1): 173-180.
Published: 08 May 2002
... the T-antigen disaccharide Galβ1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin—α-methyl-mannose complex at 2Å...
Articles
Biochem J (2002) 361 (3): 481-488.
Published: 25 January 2002
..., London ©2002 2002 BIAcore ENaC surface plasmon resonance Abbreviations used: BPTI, bovine pancreatic trypsin inhibitor; ENaC, epithelial Na + channel; hENaC, human ENaC; αhENaC, βhENaC and γhENaC, α, β and γ subunits of hENaC; GST, glutathione S-transferase; PY motif, PPXY motif; Nedd4...
Articles
Biochem J (2001) 359 (3): 679-685.
Published: 25 October 2001
... mechanisms by which Ca 2+ mediates the membrane binding of C2 domains, we measured the membrane binding of the C2 domains of group IV cytosolic phospholipase A 2 (cPLA 2 ) and protein kinase C-α (PKC-α) by surface plasmon resonance and lipid monolayer analyses. Ca 2+ ions mainly slow the membrane...
Articles
Biochem J (2000) 352 (1): 191-196.
Published: 07 November 2000
... The Biochemical Society, London © 2000 2000 casein kinase 2 enzymic regulation protein phosphorylation surface plasmon resonance transmissible spongiform encephalopathies Biochem. J. (2000) 352, 191 196 (Printed in Great Britain) 191 Bovine prion protein as a modulator of protein kinase...
Articles
Biochem J (2000) 345 (3): 627-636.
Published: 25 January 2000
... correspondence should be addressed (e-mail jungbaue@;hp01.boku.ac.at). 21 7 1999 5 10 1999 15 11 1999 The Biochemical Society, London © 2000 2000 co-operativity cyclophilin heat-shock proteins real-time biological interaction assay surface plasmon resonance Biochem. J. (2000...
Articles
Biochem J (1999) 342 (3): 605-613.
Published: 05 September 1999
..., soluble and partially glycosylated protein were purified under non-denaturing conditions and the identities of mature-length aglycosyl recPrP and two cleavage fragments were determined by electrospray MS. Binding was assessed by surface plasmon resonance techniques using both direct and competitive ligand...
Articles
Biochem J (1999) 340 (2): 411-416.
Published: 25 May 1999
..., 770-8514, Japan. 2 To whom correspondence should be addressed (e-mail antibiot@postman.riken.go.jp). 23 11 1998 22 2 1999 12 3 1999 The Biochemical Society, London © 1999 1999 microtubule network programmed cell death surface plasmon resonance tubulin Biochem...
Articles
Biochem J (1999) 339 (1): 143-149.
Published: 25 March 1999
... serum transferrin (hTF) but not bovine serum transferrin (bTF). We have used surface plasmon resonance to characterize the interaction between individual TbpA and TbpB and a series of full-length human–bovine chimaeric transferrins (hbTFs) under conditions of variable saturation with iron. A comparative...
Articles
Biochem J (1999) 337 (3): 575-583.
Published: 25 January 1999
... authors contributed equally to this work. 7 9 1998 3 11 1998 16 11 1998 The Biochemical Society, London © 1999 1999 lipid binding protein kinase B signal transduction surface plasmon resonance Biochem. J. (1999) 337, 575 583 (Printed in Great Britain) 575 Role of...