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Articles

Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions

Ali Imran, Brandon S. Moyer, Ashley J. Canning, Dan Kalina, Thomas M. Duncan, Kelsey J. Moody, Aaron J. Wolfe, Michael S. Cosgrove, Liviu Movileanu

Biochem J (2021) 478 (11): 2145–2161.

https://doi.org/10.1042/BCJ20210253

Published: 11 June 2021

.... biolaye interferometry fluorescence polarization mixed lineage leukemia protein–peptide interactions SET1/MLL proteins surface plasmon resonance For MALDI-TOF mass spectroscopy of the purified peptides, 1 µl of peptide stock in H 2 O (1–100 µM) was mixed with 9 µl spotting matrix (10 mg/ml α...

View articletitled, Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions

Includes: Supplementary data

Articles

Identification of a glycan cluster in gp120 essential for irreversible HIV-1 lytic inactivation by a lectin-based recombinantly engineered protein conjugate

Bibek Parajuli, Kriti Acharya, Aakansha Nangarlia, Shiyu Zhang, Bijay Parajuli, Alexej Dick, Brendon Ngo, Cameron F. Abrams, Irwin Chaiken

Biochem J (2020) 477 (21): 4263–4280.

https://doi.org/10.1042/BCJ20200495

Published: 13 November 2020

... Society 2020 Biacore epitope glycosylation isothermal titration calorimetry protein–protein interactions surface plasmon resonance The envelope glycoprotein (Env) of HIV-1 is the important for recognition of virus by the receptor of host T-cells (CD4, CCR5 and CXCR4) [ 1 ]. The initial...

View articletitled, Identification of a glycan cluster in gp120 essential for irreversible HIV-1 lytic inactivation by a lectin-based recombinantly engineered protein conjugate

Includes: Supplementary data

Articles

AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution

Wanjun Qi, Iain W. Manfield, Stephen P. Muench, Alison Baker

Biochem J (2017) 474 (21): 3675–3687.

https://doi.org/10.1042/BCJ20170522

Published: 23 October 2017

... provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites. Using surface plasmon resonance, a tandem P1BS sequence showed ∼50-fold higher affinity for MBPAtdPHR1 (a fusion protein comprising the DNA-binding domain and coiled-coil domain of AtPHR1 fused to maltose-binding protein...

View articletitled, AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution

Includes: Supplementary data

Articles

Structure and lipid-binding properties of the kindlin-3 pleckstrin homology domain

Tao Ni, Antreas C. Kalli, Fiona B. Naughton, Luke A. Yates, Omar Naneh, Mirijam Kozorog, Gregor Anderluh, Mark S.P. Sansom, Robert J.C. Gilbert

Biochem J (2017) 474 (4): 539–556.

https://doi.org/10.1042/BCJ20160791

Published: 03 February 2017

...–β2 loop and that it binds with somewhat higher affinity to PtdIns(3,4,5)P 3 compared with PtdIns(4,5)P 2 . Surface plasmon resonance (SPR) with lipid headgroups immobilised and the PH domain as an analyte indicate affinities of 300 µM for PtdIns(3,4,5)P 3 and 1 mM for PtdIns(4,5)P 2 . In contrast...

View articletitled, Structure and lipid-binding properties of the kindlin-3 pleckstrin homology domain

Includes: Supplementary data

Articles

Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca 2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch

Dakshnamurthy Selvakumar, Marian J. Drescher, Nathan A. Deckard, Neeliyath A. Ramakrishnan, Barbara J. Morley, Dennis G. Drescher

Biochem J (2017) 474 (1): 79–104.

https://doi.org/10.1042/BCJ20160690

Published: 22 December 2016

.... Specifically, the carboxy terminus of dopamine D1A was found to directly bind t-SNARE-associated protein snapin in teleost and mammalian hair cell models by yeast two-hybrid (Y2H) and pull-down assays, and snapin directly interacts with hair cell calcium-sensor otoferlin. Surface plasmon resonance (SPR...

View articletitled, Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca 2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch

Includes: Supplementary data

Articles

Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1

Stefan Mahrhold, Tomas Bergström, Daniel Stern, Brigitte G. Dorner, Crister Åstot, Andreas Rummel

Biochem J (2016) 473 (17): 2645–2654.

https://doi.org/10.1042/BCJ20160439

Published: 30 August 2016

... dissociation rate k d resulting in an overall 12-fold decreased binding affinity in surface plasmon resonance (SPR) experiments. The delayed dissociation might provide BoNT/A more time for endocytosis into synaptic vesicles. In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding...

View articletitled, Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1

Includes: Supplementary data

Articles

New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p -cyanophenylalanine

Daniela Xhindoli, Francesca Morgera, Ursula Zinth, Roberto Rizzo, Sabrina Pacor, Alessandro Tossi

Biochem J (2015) 465 (3): 443–457.

https://doi.org/10.1042/BJ20141016

Published: 22 January 2015

... IR and surface plasmon resonance studies also indicated different modes of interaction for LL-37 and RL-37 with anionic and neutral membranes. This correlated with a distinctly different mode of bacterial membrane permeabilization, as determined using a flow cytometric method involving impermeant...

View articletitled, New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p -cyanophenylalanine

Includes: Supplementary data

Articles

Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix

Romain Salza, Franck Peysselon, Emilie Chautard, Clément Faye, Laura Moschcovich, Tali Weiss, Laure Perrin-Cocon, Vincent Lotteau, Efrat Kessler, Sylvie Ricard-Blum

Biochem J (2014) 457 (1): 137–149.

https://doi.org/10.1042/BJ20130295

Published: 10 December 2013

... in the extracellular matrix, which could provide new insights into its biological roles. We identified 17 new partners of PCPE-1 by SPR (surface plasmon resonance) imaging. PCPE-1 forms a transient complex with the β-amyloid peptide, whereas it forms high or very high affinity complexes with laminin-111 ( K D =58.8 pM...

View articletitled, Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix

Includes: Supplementary data

Articles

Bivalent and co-operative binding of complement Factor H to heparan sulfate and heparin

Sanaullah Khan, Ruodan Nan, Jayesh Gor, Barbara Mulloy, Stephen J. Perkins

Biochem J (2012) 444 (3): 417–428.

https://doi.org/10.1042/BJ20120183

Published: 29 May 2012

... glycosaminoglycan composed of sulfated S-domains and unsulfated NA-domains. To elucidate the molecular mechanism of binding of FH to glycosaminoglycans, we performed ultracentrifugation, X-ray scattering and surface plasmon resonance with FH and glycosaminoglycan fragments. Ultracentrifugation showed that FH formed...

View articletitled, Bivalent and co-operative binding of complement Factor H to heparan sulfate and heparin

Includes: Supplementary data

Articles

A conserved motif within the flexible C-terminus of the translational regulator 4E-BP is required for tight binding to the mRNA cap-binding protein eIF4E

Keum Soon Paku, Yu Umenaga, Tsunego Usui, Ai Fukuyo, Atsuo Mizuno, Yasuko In, Toshimasa Ishida, Koji Tomoo

Biochem J (2012) 441 (1): 237–245.

https://doi.org/10.1042/BJ20101481

Published: 14 December 2011

... deletion mutants of 4E-BPn ( n =1–3) subtypes were investigated by SPR (surface plasmon resonance) analysis and ITC (isothermal titration calorimetry). Consequently, the conserved PGVTS/T motif within the C-terminal region was shown to act as the second binding region and to play an important role...

View articletitled, A conserved motif within the flexible C-terminus of the translational regulator 4E-BP is required for tight binding to the mRNA cap-binding protein eIF4E

Articles

Structural changes in the BH3 domain of SOUL protein upon interaction with the anti-apoptotic protein Bcl-xL

Emmanuele Ambrosi, Stefano Capaldi, Michele Bovi, Gianmaria Saccomani, Massimiliano Perduca, Hugo L. Monaco

Biochem J (2011) 438 (2): 291–301.

https://doi.org/10.1042/BJ20110257

Published: 12 August 2011

... identified in the protein, thus making it a potential new member of the BH3-only protein family. In the present study, we provide NMR, SPR (surface plasmon resonance) and crystallographic evidence that a peptide spanning residues 147–172 in SOUL interacts with the anti-apoptotic protein Bcl-xL. We have...

View articletitled, Structural changes in the BH3 domain of SOUL protein upon interaction with the anti-apoptotic protein Bcl-xL

Includes: Supplementary data

Articles

Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor

Christopher J. Duff, Martin J. Scott, Ian T. Kirby, Sue E. Hutchinson, Steve L. Martin, Nigel M. Hooper

Biochem J (2009) 419 (3): 577–584.

https://doi.org/10.1042/BJ20082407

Published: 14 April 2009

... against PCSK9. Studies using surface plasmon resonance demonstrated that direct binding of PCSK9 to the LDLR could be abolished with three different anti-PCSK9 antibodies. Two of these antibodies were raised against peptide epitopes in a region of the catalytic domain of PCSK9 that is involved...

View articletitled, Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor

Articles

Single-Myb-histone proteins from Arabidopsis thaliana : a quantitative study of telomere-binding specificity and kinetics

Ctirad Hofr, Pavla Šultesová, Michal Zimmermann, Iva Mozgová, Petra Procházková Schrumpfová, Michaela Wimmerová, Jiří Fajkus

Biochem J (2009) 419 (1): 221–230.

https://doi.org/10.1042/BJ20082195

Published: 13 March 2009

... quantitative DNA-binding study of this plant-specific family of proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics...

View articletitled, Single-Myb-histone proteins from Arabidopsis thaliana : a quantitative study of telomere-binding specificity and kinetics

Includes: Supplementary data

Articles

Membrane binding of zebrafish actinoporin-like protein: AF domains, a novel superfamily of cell membrane binding domains

Ion Gutiérrez-Aguirre, Peter Trontelj, Peter Maček, Jeremy H. Lakey, Gregor Anderluh

Biochem J (2006) 398 (3): 381–392.

https://doi.org/10.1042/BJ20060206

Published: 29 August 2006

... should be addressed (email [email protected] ). 3 2 2006 22 5 2006 1 6 2006 1 6 2006 The Biochemical Society, London 2006 actinoporins AF domain fungal fruit-body lectins surface plasmon resonance lipid monolayer The protein world is classically...

View articletitled, Membrane binding of zebrafish actinoporin-like protein: AF domains, a novel superfamily of cell membrane binding domains

Articles

Synaptic vesicle chips to assay botulinum neurotoxins

Géraldine Ferracci, Raymond Miquelis, Shunji Kozaki, Michael Seagar, Christian Lévêque

Biochem J (2005) 391 (3): 659–666.

https://doi.org/10.1042/BJ20050855

Published: 25 October 2005

... for rapid and sensitive in vitro assays. Here, we present new automatized bioassays to detect VAMP cleavage by BoNT/B and F. Western blotting and SPR (surface plasmon resonance) methods revealed that BoNT/B and F totally cleave their substrate on immunoisolated SVs (synaptic vesicles). Real-time monitoring...

View articletitled, Synaptic vesicle chips to assay botulinum neurotoxins

Articles

Controlled alteration of the shape and conformational stability of α-helical cell-lytic peptides: effect on mode of action and cell specificity

Igor Zelezetsky, Sabrina Pacor, Ulrike Pag, Niv Papo, Yechiel Shai, Hans-Georg Sahl, Alessandro Tossi

Biochem J (2005) 390 (1): 177–188.

https://doi.org/10.1042/BJ20042138

Published: 09 August 2005

... and surface plasmon resonance studies with model phosphatidylcholine/cholesterol membranes. The mode of interaction changes radically for a peptide with a stable, preformed helical conformation compared with others that form a structure only on membrane binding. The close correlation between effects observed...

View articletitled, Controlled alteration of the shape and conformational stability of α-helical cell-lytic peptides: effect on mode of action and cell specificity

Includes: Supplementary data

Articles

The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27 Kip1 are functional homologues with a structurally conserved inhibitory domain

Matteo BARBERIS, Luca DE GIOIA, Maria RUZZENE, Stefania SARNO, Paola COCCETTI, Piercarlo FANTUCCI, Marco VANONI, Lilia ALBERGHINA

Biochem J (2005) 387 (3): 639–647.

https://doi.org/10.1042/BJ20041299

Published: 26 April 2005

... together, these findings strongly indicate that Sic1 from budding yeast and mammalian p27 Kip1 are functional homologues with a structurally conserved inhibitory domain. cell division cycle cyclin-dependent kinase inhibitor (Cki) molecular modelling p27 Kip1 Sic1 surface plasmon resonance...

View articletitled, The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27 Kip1 are functional homologues with a structurally conserved inhibitory domain

Articles

Kinetic and structural analysis of the increased affinity of enoyl-ACP (acyl-carrier protein) reductase for triclosan in the presence of NAD +

Mili KAPOOR, P. L. Swarna MUKHI, Namita SUROLIA, K. SUGUNA, Avadhesha SUROLIA

Biochem J (2004) 381 (3): 725–733.

https://doi.org/10.1042/BJ20040228

Published: 27 July 2004

...Mili KAPOOR; P. L. Swarna MUKHI; Namita SUROLIA; K. SUGUNA; Avadhesha SUROLIA The binding of enoyl-ACP (acyl-carrier protein) reductase from Plasmodium falciparum (PfENR) with its substrates and inhibitors has been analysed by SPR (surface plasmon resonance). The binding of the substrate analogue...

View articletitled, Kinetic and structural analysis of the increased affinity of enoyl-ACP (acyl-carrier protein) reductase for triclosan in the presence of NAD +

Includes: Supplementary data

Articles

Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif

Randall J. KIMPLE, Francis S. WILLARD, Melinda D. HAINS, Miller B. JONES, Gift K. NWEKE, David P. SIDEROVSKI

Biochem J (2004) 378 (3): 801–808.

https://doi.org/10.1042/bj20031686

Published: 15 March 2004

[email protected] ). 4 11 2003 2 12 2003 5 12 2003 5 12 2003 The Biochemical Society, London ©2004 2004 fluorescence spectroscopy GoLoco motif G-protein regulatory motif (GPR motif) guanine nucleotide dissociation inhibitor heterotrimeric G-protein surface plasmon resonance...

View articletitled, Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif

Articles

Eukaryotic translation-initiation factor eIF2β binds to protein kinase CK2: effects on CK2α activity

Franc LLORENS, Nerea ROHER, Francesc A. MIRÓ, Stefania SARNO, F. Xavier RUIZ, Flavio MEGGIO, Maria PLANA, Lorenzo A. PINNA, Emilio ITARTE

Biochem J (2003) 375 (3): 623–631.

https://doi.org/10.1042/bj20030915

Published: 01 November 2003

... techniques. Surface plasmon resonance analysis indicated a high affinity in the interaction between eIF2β and CK2α, whereas the affinity for the association with CK2β is much lower. Free CK2α is unable to phosphorylate eIF2β, whereas up to 1.2 mol of phosphate/mol of eIF2β was incorporated...

View articletitled, Eukaryotic translation-initiation factor eIF2β binds to protein kinase CK2: effects on CK2α activity

Articles

Epitope mapping for the monoclonal antibody that inhibits intramolecular electron transfer in flavocytochrome b2

K. H. Diêp LÊ, Martine MAYER, Florence LEDERER

Biochem J (2003) 373 (1): 115–123.

https://doi.org/10.1042/bj20030024

Published: 01 July 2003

... inhibition of cytochrome c reduction by flavocytochrome b 2 , competitive ELISA tests and surface plasmon resonance. We have thus defined a minimal epitope surface on the haem domain; it encompasses positions 63, 64, 65, 67, 69 and 70 and one or both haem propionates. When the haem and flavin domains...

View articletitled, Epitope mapping for the monoclonal antibody that inhibits intramolecular electron transfer in flavocytochrome b2

Articles

Activation of C-terminal Src kinase (Csk) by phosphorylation at serine-364 depends on the Csk-Src homology 3 domain

Sheraz YAQUB, Hilde ABRAHAMSEN, Bastian ZIMMERMAN, Natalya KHOLOD, Knut Martin TORGERSEN, Tomas MUSTELIN, Friedrich W. HERBERG, Kjetil TASKÉN, Torkel VANG

Biochem J (2003) 372 (1): 271–278.

https://doi.org/10.1042/bj20030021

Published: 15 May 2003

... and kinase domains was facilitated by PKA-mediated phosphorylation of the kinase domain, as evaluated by surface plasmon resonance. This suggests that an overall structural domain organization and interaction between the kinase and SH3 domains are important for the activity of Csk and its regulation by PKA...

View articletitled, Activation of C-terminal Src kinase (Csk) by phosphorylation at serine-364 depends on the Csk-Src homology 3 domain

Articles

Oligolysine-based saccharide clusters: synthesis and specificity

Natacha FRISON, Philippe MARCEAU, Annie-Claude ROCHE, Michel MONSIGNY, Roger MAYER

Biochem J (2002) 368 (1): 111–119.

https://doi.org/10.1042/bj20020673

Published: 15 November 2002

... to homogeneous glycoclusters. The thiol group of the cysteine residue was used to tag the glycosylated oligolysines upon reaction with fluorescein iodoacetamide. The affinity of these glycoclusters towards two plant lectins was assessed by surface plasmon resonance. The selectivity of their cell uptake...

View articletitled, Oligolysine-based saccharide clusters: synthesis and specificity

Articles

The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies

Frank DIETZ, Sebastian FRANKEN, Kenya YOSHIDA, Hideji NAKAMURA, Joachim KAPPLER, Volkmar GIESELMANN

Biochem J (2002) 366 (2): 491–500.

https://doi.org/10.1042/bj20011811

Published: 01 September 2002

... bacterial protein expression glycosaminoglycan mitogen nuclear localization surface plasmon resonance Abbreviations used: FCS, foetal calf serum; FGF, fibroblast growth factor; GAG, glycosaminoglycan; GFP, green fluorescent protein; HDGF, hepatoma-derived growth factor; hath, homologous...

View articletitled, The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies

Articles

Studies on the relevance of the glycan at Asn-52 of the α-subunit of human chorionic gonadotropin in the αβ dimer

Paul J.A. ERBEL, Simon R. HASELEY, Johannis P. KAMERLING, Johannes F.G. VLIEGENTHART

Biochem J (2002) 364 (2): 485–495.

https://doi.org/10.1042/bj20011482

Published: 01 June 2002

... ). 12 10 2001 4 2 2002 13 3 2002 The Biochemical Society, London ©2002 2002 glycoprotein structure glycosylation hCG surface plasmon resonance Abbreviations used: 2D, two-dimensional; hCG, human chorionic gonadotropin; αhCG[glycan 52,78 ], native-hCG-derived α-subunit...

View articletitled, Studies on the relevance of the glycan at Asn-52 of the α-subunit of human chorionic gonadotropin in the αβ dimer

Articles

Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose

Yves BOURNE, Corinne Houlès ASTOUL, Véronique ZAMBONI, Willy J. PEUMANS, Laurence MENU-BOUAOUICHE, Els J.M. Van DAMME, Annick BARRE, Pierre ROUGÉ

Biochem J (2002) 364 (1): 173–180.

https://doi.org/10.1042/bj3640173

Published: 08 May 2002

... the T-antigen disaccharide Galβ1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin—α-methyl-mannose complex at 2Å...

View articletitled, Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose

Articles

A single WW domain is the predominant mediator of the interaction between the human ubiquitin-protein ligase Nedd4 and the human epithelial sodium channel

J. Shaun LOTT, Sarah J. CODDINGTON-LAWSON, Paul H. TEESDALE-SPITTLE, Fiona J. MCDONALD

Biochem J (2002) 361 (3): 481–488.

https://doi.org/10.1042/bj3610481

Published: 25 January 2002

..., London ©2002 2002 BIAcore ENaC surface plasmon resonance Abbreviations used: BPTI, bovine pancreatic trypsin inhibitor; ENaC, epithelial Na + channel; hENaC, human ENaC; αhENaC, βhENaC and γhENaC, α, β and γ subunits of hENaC; GST, glutathione S-transferase; PY motif, PPXY motif; Nedd4...

View articletitled, A single WW domain is the predominant mediator of the interaction between the human ubiquitin-protein ligase Nedd4 and the human epithelial sodium channel

Articles

Roles of calcium ions in the membrane binding of C2 domains

Robert V. STAHELIN, Wonhwa CHO

Biochem J (2001) 359 (3): 679–685.

https://doi.org/10.1042/bj3590679

Published: 25 October 2001

... the mechanisms by which Ca 2+ mediates the membrane binding of C2 domains, we measured the membrane binding of the C2 domains of group IV cytosolic phospholipase A 2 (cPLA 2 ) and protein kinase C-α (PKC-α) by surface plasmon resonance and lipid monolayer analyses. Ca 2+ ions mainly slow the membrane...

View articletitled, Roles of calcium ions in the membrane binding of C2 domains

Articles

Bovine prion protein as a modulator of protein kinase CK2

Flavio MEGGIO, Alessandro NEGRO, Stefania SARNO, Maria RUZZENE, Alessandro BERTOLI, M. Catia SORGATO, Lorenzo A. PINNA

Biochem J (2000) 352 (1): 191–196.

https://doi.org/10.1042/bj3520191

Published: 07 November 2000

...; CK2, casein kinase 2 ; I-2, inhibitor 2 of protein phosphatase 1; PrP, prion protein ; PrPc, cellular PrP; PrPSc, pathological isoform of PrP; SPR, surface plasmon resonance. 1 To whom correspondence should be addressed (e-mail pinna!civ.bio.unipd.it). not affect its catalytic activity. The opposite...

View articletitled, Bovine prion protein as a modulator of protein kinase CK2

Articles

Quantitative assessment of complex formation of nuclear-receptor accessory proteins

Klaus GRAUMANN, Alois JUNGBAUER

Biochem J (2000) 345 (3): 627–636.

https://doi.org/10.1042/bj3450627

Published: 25 January 2000

... correspondence should be addressed (e-mail jungbaue@;hp01.boku.ac.at). 21 7 1999 5 10 1999 15 11 1999 The Biochemical Society, London © 2000 2000 co-operativity cyclophilin heat-shock proteins real-time biological interaction assay surface plasmon resonance Biochem. J. (2000...

View articletitled, Quantitative assessment of complex formation of nuclear-receptor accessory proteins

Articles

Characterization and polyanion-binding properties of purified recombinant prion protein

Debbie B. BRIMACOMBE, Alan D. BENNETT, Fred S. WUSTEMAN, Andrew C. GILL, Janine C. DANN, Christopher J. BOSTOCK

Biochem J (1999) 342 (3): 605–613.

https://doi.org/10.1042/bj3420605

Published: 05 September 1999

..., soluble and partially glycosylated protein were purified under non-denaturing conditions and the identities of mature-length aglycosyl recPrP and two cleavage fragments were determined by electrospray MS. Binding was assessed by surface plasmon resonance techniques using both direct and competitive ligand...

View articletitled, Characterization and polyanion-binding properties of purified recombinant prion protein

Articles

Apoptosis induction via microtubule disassembly by an antitumour compound, pironetin

Masuo KONDOH, Takeo USUI, Takaaki NISHIKIORI, Tadanori MAYUMI, Hiroyuki OSADA

Biochem J (1999) 340 (2): 411–416.

https://doi.org/10.1042/bj3400411

Published: 25 May 1999

..., Japan. 2 To whom correspondence should be addressed (e-mail [email protected]). 23 11 1998 22 2 1999 12 3 1999 The Biochemical Society, London © 1999 1999 microtubule network programmed cell death surface plasmon resonance tubulin Biochem. J. (1999...

View articletitled, Apoptosis induction via microtubule disassembly by an antitumour compound, pironetin

Articles

Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin

Ian C. BOULTON, Andrew R. GORRINGE, Beatrice GORINSKY, Mark D. RETZER, Anthony B. SCHRYVERS, Christopher L. JOANNOU, Robert W. EVANS

Biochem J (1999) 339 (1): 143–149.

https://doi.org/10.1042/bj3390143

Published: 25 March 1999

... serum transferrin (hTF) but not bovine serum transferrin (bTF). We have used surface plasmon resonance to characterize the interaction between individual TbpA and TbpB and a series of full-length human–bovine chimaeric transferrins (hbTFs) under conditions of variable saturation with iron. A comparative...

View articletitled, Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin

Articles

Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1

Richard A. CURRIE, Kay S. WALKER, Alex GRAY, Maria DEAK, Antonio CASAMAYOR, C. Peter DOWNES, Philip COHEN, Dario R. ALESSI, John LUCOCQ

Biochem J (1999) 337 (3): 575–583.

https://doi.org/10.1042/bj3370575

Published: 25 January 1999

... contributed equally to this work. 7 9 1998 3 11 1998 16 11 1998 The Biochemical Society, London © 1999 1999 lipid binding protein kinase B signal transduction surface plasmon resonance Biochem. J. (1999) 337, 575 583 (Printed in Great Britain) 575 Role...

View articletitled, Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1

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