Skip Nav Destination
Close Modal
Update search
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
NARROW
Format
Subjects
Article Type
Date
Availability
1-34 of 34
Keywords: surface plasmon resonance
Close
Follow your search
Access your saved searches in your account
Would you like to receive an alert when new items match your search?
Sort by
Articles
Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions
Available to PurchaseAli Imran, Brandon S. Moyer, Ashley J. Canning, Dan Kalina, Thomas M. Duncan, Kelsey J. Moody, Aaron J. Wolfe, Michael S. Cosgrove, Liviu Movileanu
Journal:
Biochemical Journal
Biochem J (2021) 478 (11): 2145–2161.
Published: 11 June 2021
.... biolaye interferometry fluorescence polarization mixed lineage leukemia protein–peptide interactions SET1/MLL proteins surface plasmon resonance For MALDI-TOF mass spectroscopy of the purified peptides, 1 µl of peptide stock in H 2 O (1–100 µM) was mixed with 9 µl spotting matrix (10 mg/ml α...
Includes: Supplementary data
Articles
Identification of a glycan cluster in gp120 essential for irreversible HIV-1 lytic inactivation by a lectin-based recombinantly engineered protein conjugate
Available to PurchaseBibek Parajuli, Kriti Acharya, Aakansha Nangarlia, Shiyu Zhang, Bijay Parajuli, Alexej Dick, Brendon Ngo, Cameron F. Abrams, Irwin Chaiken
Journal:
Biochemical Journal
Biochem J (2020) 477 (21): 4263–4280.
Published: 13 November 2020
... Society 2020 Biacore epitope glycosylation isothermal titration calorimetry protein–protein interactions surface plasmon resonance The envelope glycoprotein (Env) of HIV-1 is the important for recognition of virus by the receptor of host T-cells (CD4, CCR5 and CXCR4) [ 1 ]. The initial...
Includes: Supplementary data
Articles
AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution
Open Access
Journal:
Biochemical Journal
Biochem J (2017) 474 (21): 3675–3687.
Published: 23 October 2017
... provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites. Using surface plasmon resonance, a tandem P1BS sequence showed ∼50-fold higher affinity for MBPAtdPHR1 (a fusion protein comprising the DNA-binding domain and coiled-coil domain of AtPHR1 fused to maltose-binding protein...
Includes: Supplementary data
Articles
Tao Ni, Antreas C. Kalli, Fiona B. Naughton, Luke A. Yates, Omar Naneh, Mirijam Kozorog, Gregor Anderluh, Mark S.P. Sansom, Robert J.C. Gilbert
Journal:
Biochemical Journal
Biochem J (2017) 474 (4): 539–556.
Published: 03 February 2017
...–β2 loop and that it binds with somewhat higher affinity to PtdIns(3,4,5)P 3 compared with PtdIns(4,5)P 2 . Surface plasmon resonance (SPR) with lipid headgroups immobilised and the PH domain as an analyte indicate affinities of 300 µM for PtdIns(3,4,5)P 3 and 1 mM for PtdIns(4,5)P 2 . In contrast...
Includes: Supplementary data
Articles
Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca 2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
Available to PurchaseDakshnamurthy Selvakumar, Marian J. Drescher, Nathan A. Deckard, Neeliyath A. Ramakrishnan, Barbara J. Morley, Dennis G. Drescher
Journal:
Biochemical Journal
Biochem J (2017) 474 (1): 79–104.
Published: 22 December 2016
.... Specifically, the carboxy terminus of dopamine D1A was found to directly bind t-SNARE-associated protein snapin in teleost and mammalian hair cell models by yeast two-hybrid (Y2H) and pull-down assays, and snapin directly interacts with hair cell calcium-sensor otoferlin. Surface plasmon resonance (SPR...
Includes: Supplementary data
Articles
Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2016) 473 (17): 2645–2654.
Published: 30 August 2016
... dissociation rate k d resulting in an overall 12-fold decreased binding affinity in surface plasmon resonance (SPR) experiments. The delayed dissociation might provide BoNT/A more time for endocytosis into synaptic vesicles. In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding...
Includes: Supplementary data
Articles
New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p -cyanophenylalanine
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2015) 465 (3): 443–457.
Published: 22 January 2015
... IR and surface plasmon resonance studies also indicated different modes of interaction for LL-37 and RL-37 with anionic and neutral membranes. This correlated with a distinctly different mode of bacterial membrane permeabilization, as determined using a flow cytometric method involving impermeant...
Includes: Supplementary data
Articles
Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix
Available to PurchaseRomain Salza, Franck Peysselon, Emilie Chautard, Clément Faye, Laura Moschcovich, Tali Weiss, Laure Perrin-Cocon, Vincent Lotteau, Efrat Kessler, Sylvie Ricard-Blum
Journal:
Biochemical Journal
Biochem J (2014) 457 (1): 137–149.
Published: 10 December 2013
... in the extracellular matrix, which could provide new insights into its biological roles. We identified 17 new partners of PCPE-1 by SPR (surface plasmon resonance) imaging. PCPE-1 forms a transient complex with the β-amyloid peptide, whereas it forms high or very high affinity complexes with laminin-111 ( K D =58.8 pM...
Includes: Supplementary data
Articles
Bivalent and co-operative binding of complement Factor H to heparan sulfate and heparin
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2012) 444 (3): 417–428.
Published: 29 May 2012
... glycosaminoglycan composed of sulfated S-domains and unsulfated NA-domains. To elucidate the molecular mechanism of binding of FH to glycosaminoglycans, we performed ultracentrifugation, X-ray scattering and surface plasmon resonance with FH and glycosaminoglycan fragments. Ultracentrifugation showed that FH formed...
Includes: Supplementary data
Articles
A conserved motif within the flexible C-terminus of the translational regulator 4E-BP is required for tight binding to the mRNA cap-binding protein eIF4E
Available to PurchaseKeum Soon Paku, Yu Umenaga, Tsunego Usui, Ai Fukuyo, Atsuo Mizuno, Yasuko In, Toshimasa Ishida, Koji Tomoo
Journal:
Biochemical Journal
Biochem J (2012) 441 (1): 237–245.
Published: 14 December 2011
... deletion mutants of 4E-BPn ( n =1–3) subtypes were investigated by SPR (surface plasmon resonance) analysis and ITC (isothermal titration calorimetry). Consequently, the conserved PGVTS/T motif within the C-terminal region was shown to act as the second binding region and to play an important role...
Articles
Structural changes in the BH3 domain of SOUL protein upon interaction with the anti-apoptotic protein Bcl-xL
Available to PurchaseEmmanuele Ambrosi, Stefano Capaldi, Michele Bovi, Gianmaria Saccomani, Massimiliano Perduca, Hugo L. Monaco
Journal:
Biochemical Journal
Biochem J (2011) 438 (2): 291–301.
Published: 12 August 2011
... identified in the protein, thus making it a potential new member of the BH3-only protein family. In the present study, we provide NMR, SPR (surface plasmon resonance) and crystallographic evidence that a peptide spanning residues 147–172 in SOUL interacts with the anti-apoptotic protein Bcl-xL. We have...
Includes: Supplementary data
Articles
Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor
Open AccessChristopher J. Duff, Martin J. Scott, Ian T. Kirby, Sue E. Hutchinson, Steve L. Martin, Nigel M. Hooper
Journal:
Biochemical Journal
Biochem J (2009) 419 (3): 577–584.
Published: 14 April 2009
... against PCSK9. Studies using surface plasmon resonance demonstrated that direct binding of PCSK9 to the LDLR could be abolished with three different anti-PCSK9 antibodies. Two of these antibodies were raised against peptide epitopes in a region of the catalytic domain of PCSK9 that is involved...
Articles
Single-Myb-histone proteins from Arabidopsis thaliana : a quantitative study of telomere-binding specificity and kinetics
Available to PurchaseCtirad Hofr, Pavla Šultesová, Michal Zimmermann, Iva Mozgová, Petra Procházková Schrumpfová, Michaela Wimmerová, Jiří Fajkus
Journal:
Biochemical Journal
Biochem J (2009) 419 (1): 221–230.
Published: 13 March 2009
... quantitative DNA-binding study of this plant-specific family of proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics...
Includes: Supplementary data
Articles
Membrane binding of zebrafish actinoporin-like protein: AF domains, a novel superfamily of cell membrane binding domains
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2006) 398 (3): 381–392.
Published: 29 August 2006
... should be addressed (email [email protected] ). 3 2 2006 22 5 2006 1 6 2006 1 6 2006 The Biochemical Society, London 2006 actinoporins AF domain fungal fruit-body lectins surface plasmon resonance lipid monolayer The protein world is classically...
Articles
Synaptic vesicle chips to assay botulinum neurotoxins
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2005) 391 (3): 659–666.
Published: 25 October 2005
... for rapid and sensitive in vitro assays. Here, we present new automatized bioassays to detect VAMP cleavage by BoNT/B and F. Western blotting and SPR (surface plasmon resonance) methods revealed that BoNT/B and F totally cleave their substrate on immunoisolated SVs (synaptic vesicles). Real-time monitoring...
Articles
Controlled alteration of the shape and conformational stability of α-helical cell-lytic peptides: effect on mode of action and cell specificity
Available to PurchaseIgor Zelezetsky, Sabrina Pacor, Ulrike Pag, Niv Papo, Yechiel Shai, Hans-Georg Sahl, Alessandro Tossi
Journal:
Biochemical Journal
Biochem J (2005) 390 (1): 177–188.
Published: 09 August 2005
... and surface plasmon resonance studies with model phosphatidylcholine/cholesterol membranes. The mode of interaction changes radically for a peptide with a stable, preformed helical conformation compared with others that form a structure only on membrane binding. The close correlation between effects observed...
Includes: Supplementary data
Articles
The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27 Kip1 are functional homologues with a structurally conserved inhibitory domain
Available to PurchaseMatteo BARBERIS, Luca DE GIOIA, Maria RUZZENE, Stefania SARNO, Paola COCCETTI, Piercarlo FANTUCCI, Marco VANONI, Lilia ALBERGHINA
Journal:
Biochemical Journal
Biochem J (2005) 387 (3): 639–647.
Published: 26 April 2005
... together, these findings strongly indicate that Sic1 from budding yeast and mammalian p27 Kip1 are functional homologues with a structurally conserved inhibitory domain. cell division cycle cyclin-dependent kinase inhibitor (Cki) molecular modelling p27 Kip1 Sic1 surface plasmon resonance...
Articles
Kinetic and structural analysis of the increased affinity of enoyl-ACP (acyl-carrier protein) reductase for triclosan in the presence of NAD +
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2004) 381 (3): 725–733.
Published: 27 July 2004
...Mili KAPOOR; P. L. Swarna MUKHI; Namita SUROLIA; K. SUGUNA; Avadhesha SUROLIA The binding of enoyl-ACP (acyl-carrier protein) reductase from Plasmodium falciparum (PfENR) with its substrates and inhibitors has been analysed by SPR (surface plasmon resonance). The binding of the substrate analogue...
Includes: Supplementary data
Articles
Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif
Available to PurchaseRandall J. KIMPLE, Francis S. WILLARD, Melinda D. HAINS, Miller B. JONES, Gift K. NWEKE, David P. SIDEROVSKI
Journal:
Biochemical Journal
Biochem J (2004) 378 (3): 801–808.
Published: 15 March 2004
[email protected] ). 4 11 2003 2 12 2003 5 12 2003 5 12 2003 The Biochemical Society, London ©2004 2004 fluorescence spectroscopy GoLoco motif G-protein regulatory motif (GPR motif) guanine nucleotide dissociation inhibitor heterotrimeric G-protein surface plasmon resonance...
Articles
Eukaryotic translation-initiation factor eIF2β binds to protein kinase CK2: effects on CK2α activity
Available to PurchaseFranc LLORENS, Nerea ROHER, Francesc A. MIRÓ, Stefania SARNO, F. Xavier RUIZ, Flavio MEGGIO, Maria PLANA, Lorenzo A. PINNA, Emilio ITARTE
Journal:
Biochemical Journal
Biochem J (2003) 375 (3): 623–631.
Published: 01 November 2003
... techniques. Surface plasmon resonance analysis indicated a high affinity in the interaction between eIF2β and CK2α, whereas the affinity for the association with CK2β is much lower. Free CK2α is unable to phosphorylate eIF2β, whereas up to 1.2 mol of phosphate/mol of eIF2β was incorporated...
Articles
Epitope mapping for the monoclonal antibody that inhibits intramolecular electron transfer in flavocytochrome b2
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2003) 373 (1): 115–123.
Published: 01 July 2003
... inhibition of cytochrome c reduction by flavocytochrome b 2 , competitive ELISA tests and surface plasmon resonance. We have thus defined a minimal epitope surface on the haem domain; it encompasses positions 63, 64, 65, 67, 69 and 70 and one or both haem propionates. When the haem and flavin domains...
Articles
Activation of C-terminal Src kinase (Csk) by phosphorylation at serine-364 depends on the Csk-Src homology 3 domain
Available to PurchaseSheraz YAQUB, Hilde ABRAHAMSEN, Bastian ZIMMERMAN, Natalya KHOLOD, Knut Martin TORGERSEN, Tomas MUSTELIN, Friedrich W. HERBERG, Kjetil TASKÉN, Torkel VANG
Journal:
Biochemical Journal
Biochem J (2003) 372 (1): 271–278.
Published: 15 May 2003
... and kinase domains was facilitated by PKA-mediated phosphorylation of the kinase domain, as evaluated by surface plasmon resonance. This suggests that an overall structural domain organization and interaction between the kinase and SH3 domains are important for the activity of Csk and its regulation by PKA...
Articles
Oligolysine-based saccharide clusters: synthesis and specificity
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2002) 368 (1): 111–119.
Published: 15 November 2002
... to homogeneous glycoclusters. The thiol group of the cysteine residue was used to tag the glycosylated oligolysines upon reaction with fluorescein iodoacetamide. The affinity of these glycoclusters towards two plant lectins was assessed by surface plasmon resonance. The selectivity of their cell uptake...
Articles
The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2002) 366 (2): 491–500.
Published: 01 September 2002
... bacterial protein expression glycosaminoglycan mitogen nuclear localization surface plasmon resonance Abbreviations used: FCS, foetal calf serum; FGF, fibroblast growth factor; GAG, glycosaminoglycan; GFP, green fluorescent protein; HDGF, hepatoma-derived growth factor; hath, homologous...
Articles
Studies on the relevance of the glycan at Asn-52 of the α-subunit of human chorionic gonadotropin in the αβ dimer
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2002) 364 (2): 485–495.
Published: 01 June 2002
... ). 12 10 2001 4 2 2002 13 3 2002 The Biochemical Society, London ©2002 2002 glycoprotein structure glycosylation hCG surface plasmon resonance Abbreviations used: 2D, two-dimensional; hCG, human chorionic gonadotropin; αhCG[glycan 52,78 ], native-hCG-derived α-subunit...
Articles
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose
Available to PurchaseYves BOURNE, Corinne Houlès ASTOUL, Véronique ZAMBONI, Willy J. PEUMANS, Laurence MENU-BOUAOUICHE, Els J.M. Van DAMME, Annick BARRE, Pierre ROUGÉ
Journal:
Biochemical Journal
Biochem J (2002) 364 (1): 173–180.
Published: 08 May 2002
... the T-antigen disaccharide Galβ1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin—α-methyl-mannose complex at 2Å...
Articles
A single WW domain is the predominant mediator of the interaction between the human ubiquitin-protein ligase Nedd4 and the human epithelial sodium channel
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2002) 361 (3): 481–488.
Published: 25 January 2002
..., London ©2002 2002 BIAcore ENaC surface plasmon resonance Abbreviations used: BPTI, bovine pancreatic trypsin inhibitor; ENaC, epithelial Na + channel; hENaC, human ENaC; αhENaC, βhENaC and γhENaC, α, β and γ subunits of hENaC; GST, glutathione S-transferase; PY motif, PPXY motif; Nedd4...
Articles
Roles of calcium ions in the membrane binding of C2 domains
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2001) 359 (3): 679–685.
Published: 25 October 2001
... the mechanisms by which Ca 2+ mediates the membrane binding of C2 domains, we measured the membrane binding of the C2 domains of group IV cytosolic phospholipase A 2 (cPLA 2 ) and protein kinase C-α (PKC-α) by surface plasmon resonance and lipid monolayer analyses. Ca 2+ ions mainly slow the membrane...
Articles
Bovine prion protein as a modulator of protein kinase CK2
Available to PurchaseFlavio MEGGIO, Alessandro NEGRO, Stefania SARNO, Maria RUZZENE, Alessandro BERTOLI, M. Catia SORGATO, Lorenzo A. PINNA
Journal:
Biochemical Journal
Biochem J (2000) 352 (1): 191–196.
Published: 07 November 2000
...; CK2, casein kinase 2 ; I-2, inhibitor 2 of protein phosphatase 1; PrP, prion protein ; PrPc, cellular PrP; PrPSc, pathological isoform of PrP; SPR, surface plasmon resonance. 1 To whom correspondence should be addressed (e-mail pinna!civ.bio.unipd.it). not affect its catalytic activity. The opposite...
Articles
Quantitative assessment of complex formation of nuclear-receptor accessory proteins
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 345 (3): 627–636.
Published: 25 January 2000
... correspondence should be addressed (e-mail jungbaue@;hp01.boku.ac.at). 21 7 1999 5 10 1999 15 11 1999 The Biochemical Society, London © 2000 2000 co-operativity cyclophilin heat-shock proteins real-time biological interaction assay surface plasmon resonance Biochem. J. (2000...
Articles
Characterization and polyanion-binding properties of purified recombinant prion protein
Available to PurchaseDebbie B. BRIMACOMBE, Alan D. BENNETT, Fred S. WUSTEMAN, Andrew C. GILL, Janine C. DANN, Christopher J. BOSTOCK
Journal:
Biochemical Journal
Biochem J (1999) 342 (3): 605–613.
Published: 05 September 1999
..., soluble and partially glycosylated protein were purified under non-denaturing conditions and the identities of mature-length aglycosyl recPrP and two cleavage fragments were determined by electrospray MS. Binding was assessed by surface plasmon resonance techniques using both direct and competitive ligand...
Articles
Apoptosis induction via microtubule disassembly by an antitumour compound, pironetin
Available to Purchase
Journal:
Biochemical Journal
Biochem J (1999) 340 (2): 411–416.
Published: 25 May 1999
..., Japan. 2 To whom correspondence should be addressed (e-mail [email protected]). 23 11 1998 22 2 1999 12 3 1999 The Biochemical Society, London © 1999 1999 microtubule network programmed cell death surface plasmon resonance tubulin Biochem. J. (1999...
Articles
Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin
Available to PurchaseIan C. BOULTON, Andrew R. GORRINGE, Beatrice GORINSKY, Mark D. RETZER, Anthony B. SCHRYVERS, Christopher L. JOANNOU, Robert W. EVANS
Journal:
Biochemical Journal
Biochem J (1999) 339 (1): 143–149.
Published: 25 March 1999
... serum transferrin (hTF) but not bovine serum transferrin (bTF). We have used surface plasmon resonance to characterize the interaction between individual TbpA and TbpB and a series of full-length human–bovine chimaeric transferrins (hbTFs) under conditions of variable saturation with iron. A comparative...
Articles
Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
Available to PurchaseRichard A. CURRIE, Kay S. WALKER, Alex GRAY, Maria DEAK, Antonio CASAMAYOR, C. Peter DOWNES, Philip COHEN, Dario R. ALESSI, John LUCOCQ
Journal:
Biochemical Journal
Biochem J (1999) 337 (3): 575–583.
Published: 25 January 1999
... contributed equally to this work. 7 9 1998 3 11 1998 16 11 1998 The Biochemical Society, London © 1999 1999 lipid binding protein kinase B signal transduction surface plasmon resonance Biochem. J. (1999) 337, 575 583 (Printed in Great Britain) 575 Role...