...Petr Fajkus; Jiří Fajkus Telomeres, essential for maintaining genomic stability, are typically preserved through the action of telomerase, a ribonucleoprotein complex that synthesizes telomeric DNA. One of its two core components, telomerase RNA (TR), serves as the template for this synthesis...

... . Recombinant RECQ1 significantly stimulated FEN-1 endonucleolytic cleavage of 5′-flap DNA substrates containing non-telomeric or telomeric repeat sequence. RECQ1 and FEN-1 were constitutively present at telomeres and their binding to the telomeric chromatin was enhanced following DNA damage. Telomere residence...

...Prabhat Khadka; Ji Hoon Lee; Seung Han Baek; Sue Young Oh; In Kwon Chung Human telomeres associate with shelterin, a six-protein complex that protects chromosome ends from being recognized as sites of DNA damage. The shelterin subunit TRF2 (telomeric repeat-binding factor 2) protects telomeres...

...Yi-Ching Hsieh; Pei-Jung Tu; Ying-Yuan Lee; Chun-Chen Kuo; Yi-Chien Lin; Chi-Fang Wu; Jing-Jer Lin Imp4p is a component of U3 snoRNP (small nucleolar ribonucleoprotein) involved in the maturation of 18S rRNA. We have shown that Imp4p interacts with Cdc13p, a single-stranded telomere-binding protein...

... in a processive manner, but could not translocate to synthesize more than one telomeric repeat. The purified telomerase core complex exhibited different activities when primers were paired with the Tlc1 template at different positions. The procedure of reconstitution and purification of telomerase core enzyme...

... lysosomal-membrane integrity against H 2 O 2 -induced oxidative disruption. More importantly, both forms of DFO prevented H 2 O 2 -induced strand breaks in nuclear DNA, including telomeres. To exclude the possibility that lysosomal hydrolases, rather than iron, caused the observed DNA damage, limited...

...Jun Hyun KIM; Joo Hee KIM; Gun Eui Lee; Sang Woong KIM; I. Kwon CHUNG Telomere maintenance is essential for the continued proliferation of dividing cells, and is implicated in chromosome stability and cell immortalization. Telomerase activity allows cells to maintain their telomeric DNA...

...Manu DE RYCKER; Ranga N. VENKATESAN; Chao WEI; Carolyn M. PRICE Tankyrases 1 and 2 are two highly related poly(ADP-ribose) polymerases that interact with a variety of cytoplasmic and nuclear proteins. Both proteins have been implicated in telomere length regulation, insulin signalling...