... Cdk2 cell cycle cyclin-dependent kinases cyclins meiosis testis Cyclin-dependent kinases (Cdks) are critical regulators of cell cycle progression in eukaryotic cells via the phosphorylation of key substrates involved in DNA replication and mitosis [ 1 ]. Cdk2 regulates the G1/S-phase...

... cells. Despite the implicated role of RLF/INSL3 in testis development, its native conformation remains unknown. In the present paper we demonstrate for the first time that boar testicular RLF/INSL3 is isolated as a monomeric structure with full biological activity. Using a series of chromatography steps...

... myopathy or Charcot–Marie–Tooth disease, which manifest in skeletal muscle or in peripheral neurons respectively. Interestingly, studies have shown MTMR2 and MTMR5 , two MTM family members, to be highly expressed in the testis, particularly in Sertoli and germ cells, and knockout of either gene resulted...

... comprises two homologous domains, the differing substrate preferences of which present a new avenue for domain-selective inhibitor design. We have co-crystallized lisW-S, a C-domain-selective derivative of the drug lisinopril, with human testis ACE and determined a structure using X-ray crystallography...

..., immunolocalization microscopy and cell fractionation studies with transfected HEK-293 cells and mouse testis show that MRP9/Mrp9 does not localize to mitochondria. In HEK-293 cells, it is predominantly localized in the endoplasmic reticulum. We have been unable to demonstrate transport by MRP9 of substrates...

...Yasumasa Nishito; Mizuho Hasegawa; Naohiro Inohara; Gabriel Núñez In the present study, we report the identification and characterization of MEX ( ME KK1-related protein X ), a protein with homology to MEKK1 that is expressed uniquely in the testis. MEX is comprises four putative zinc-binding...

...% and 36.7% with mouse ABCA3 and sea urchin ABCA respectively. RNA blot and quantitative real-time PCR analyses showed that ABCA17 mRNA is expressed exclusively in the testis. Examination of testis by in situ hybridization showed that ABCA17 mRNA is expressed in germ cells, mainly spermatocytes...

...Michaela KUHN; Chi Kin Domingos NG; Yueh-Hsing SU; Ana KILIĆ; Danuta MITKO; Nga BIEN-LY; László G. KÖMÜVES; Ruey-Bing YANG We have identified a novel membrane form of guanylate cyclase (GC) from a mouse testis cDNA library and termed it mGC-G (mouse GC-G) based on its high sequence homology to rat...

... ). 22 10 2002 12 2 2003 19 2 2003 19 2 2003 The Biochemical Society, London ©2003 2003 cytokinesis RhoGAP testis Abbreviations used: COG, conserved oligomeric Golgi; ECL®, enhanced chemiluminescence; GST, glutathione S-transferase; GAP, GTPase-activating protein...

... OTEX . A phylogenetic analysis revealed that OTEX belonged to the recently defined PEPP subfamily of paired -like homeobox genes. It was organized into three introns and, like the other PEPP genes, it was mapped to chromosome X. Its transcripts were detected mainly in the ovary, testis and epididymis...

... (RNPDE7B3), which possessed unique N-terminal sequences, consisted of 359 and 459 residues respectively. Northern hybridization analysis showed that rat PDE7B transcripts were particularly abundant in the striatum and testis. PCR analyses revealed that rat PDE7B2 transcripts were restricted to the testis...

... synthesis provides a good index of NPRA protein expression, which ranges from maximal in adrenal gland, lung, kidney, and testis to minimal in heart and colon. In contrast, immunoreactive NPRA is not detectable in tissues isolated from NPRA knockout animals and ANP- and BNP-stimulatable GC activities...

... (testis- and skeletal-muscle-specific DSP). Nucleotide sequence analysis of TMDP cDNA indicated that the open reading frame of 597 bp encodes a protein of 198 amino acid residues with a predicted molecular mass of 22.5 kDa. The deduced amino acid sequence contains a motif for a conserved catalytic domain...

... testis and sperm extracts probed with a macaque anti-tMDC II polyclonal antiserum. These results are discussed in the context of our proposed model for multiple proteins implicated in sperm-egg interactions. 1 To whom correspondence should be addressed (e-mail [email protected]...

... function. Key words: epididymis, pituitary gland, reproduction, testis. broadly expressed [11]. Although it is well established by studies in Šitro that the cystatins are cysteine protease inhibitors with specificities against papain-like cysteine proteases such as cathepsins B, S, H and L, the functions...