... into homo- and hetero-tetramers. This is demonstrated in yeast, mammalian cells and native tissue by complementary yeast two-hybrid, chemical cross-linking and co-immunoprecipitation assays. The IP 3 (inositol 1,4,5-trisphosphate) receptor N-terminus (residues 1–667) similarly exhibits tetrameric...

... GTPase activity by inducing conformational changes in the bound nucleotide. In the present study we investigate the importance of the tetramerization of ZapA on its function. We generated a number of mutant forms of ZapA with the aim of disrupting the dimer–dimer interface. We show that one...

... tetramerization of G142A was significantly impaired. Our observations highlight the sensitivity of the structure of CTPS to mutations in the UTP- and CTP-binding sites, with Gly 142 being critical for nucleotide-dependent oligomerization of CTPS to active tetramers. This ‘structural sensitivity’ may limit...

...Maria E. BULINA; Vladislav V. VERKHUSHA; Dmitry B. STAROVEROV; Dmitry M. CHUDAKOV; Konstantin A. LUKYANOV The tendency for tetramerization is the main disadvantage in the green fluorescent protein homologues from Anthozoa species. We report a universal method called hetero-oligomeric tagging, which...