.... The effect of D -glucose binding on the thermal unfolding of the GGBP protein at pH 7.0 has been measured by differential scanning calorimetry (DSC), far-UV CD and intrinsic tryptophanyl residue fluorescence (Trp fluorescence). All three techniques reveal reversible, thermal transitions and a midpoint...

... electrophoresis metalloenzyme phosphotriesterase protein stability thermal unfolding Abbreviations used: BCA, bicinchoninic acid; CE, capillary electrophoresis; CHES, 2-( N -cyclohexylamino)ethanesulphonic acid; IEF, isoelectric focusing; OP, organophosphorus compounds; PTE, phosphotriesterase; RMSD...

..., is an effector that influences folding and stability of newly translocated proteins. Key words: exocellular protein, reversible folding, thermal unfolding, urea denaturation. required for a complete kinetic and thermodynamic charac- terization of conformational transition is not possible under these conditions...