... investigated the membrane topology of Ypc1p by probing the cysteine residue accessibility of natural and substituted cysteines with membrane non-permeating mass-tagged probes. The N- and C-terminal ends of Ypc1p are oriented towards the lumen and cytosol respectively. Two of the five natural cysteines, Cys 27...

...). Besides the signal peptide, amino acids 31–90 of Nrf1 also negatively regulate the CNC-bZIP factor. In the present study we have tested the hypothesis that amino acids 31–90 of Nrf1, and the overlapping NHB2 (N-terminal homology box 2; residues 82–106), inhibit Nrf1 because they control its topology...

... protein. Samples of both total and cell-surface protein were analysed by Western blotting as above. ion channel pre-transmembrane segment 2 (TM2) topology scanning cysteine mutagenesis P2X receptors are a family of ligand-gated ion channels that play key roles in diverse physiological...

... mitochondrial membrane serum- and glucocorticoid-inducible kinase 1 (Sgk1) topology The serum- and glucocorticoid-inducible serine/threonine kinase Sgk1 (serum- and glucocorticoid-inducible kinase 1) consists of 431 amino acids and belongs to a family of structurally related enzymes termed AGC-type...

... cerevisiae [Guillas, Kirchman, Chuard, Pfefferli, Jiang, Jazwinski and Conzelman (2001) EMBO J. 20 , 2655–2665; Schorling, Vallee, Barz, Reizman and Oesterhelt (2001) Mol. Biol. Cell 12 , 3417–3427; Vallee and Riezman (2005) EMBO J. 24 , 730–741]. In the present study, the topology of the Lag1p and Lac1p...

... that increases the carbon-dioxide-carrying capacity of blood. To study the topology of TMs (transmembrane segments) 1–4, a series of scanning N-glycosylation mutants were created spanning the region from EC (extracellular loop) 1 to EC2 in full-length AE1. These constructs were expressed in HEK-293 (human...

..., Lass5 and Lass6 were N-glycosylated, each at their N-terminal Asn residue. The occurrence of N-glycosylation of some Lass proteins provides topological insight, indicating that the N-termini of Lass family members probably face the luminal side of the endoplasmic reticulum membrane. Furthermore, based...

... for a topological model of the N-terminal extension on the surface of the gelsolin molecule, which was unknown previously. 1 Present address: Department of Biochemistry, University of Leicester, Leicester, U.K. 2 To whom correspondence should be addressed (email [email protected]...

...Leonardo M. CASANO; H. Ramiro LASCANO; Mercedes MARTÍN; Bartolomé SABATER We have investigated the topologies of Ndh (a plastid complex with NADH dehydrogenase activity) and its NDH-F subunit in thylakoids by trypsin and proteinase V8 digestion of both intact and Triton X-100-permeabilized barley...

.... This demonstrates that the topology of the substrate-binding region of BthTx-I has a direct effect on the Ca 2+ -independent membrane damage, and implies that substrate binding retains an important role in this process. bothropstoxin-I Lys 49 -phospholipase A 2 (Lys 49 -PLA 2 ) mutagenesis myotoxicity...

... topology twin arginine targeting Abbreviations used: LB, Luria–Bertani; MKH 2 , reduced menaquinone; Nap, periplasmic nitrate reductase; TAT, twin-arginine targeting; UQH 2 , reduced ubiquinone; X-Gal, 5-bromo-4-chloroindol-3-yl β- d -galactopyranoside; X-P, bromo-4-chloroindolyl phosphate...

... on information from the incomplete B. pseudomallei genome-sequencing project, the genes encoding Omp38 were identified and amplified by PCR from B. pseudomallei and B. thailandensis genomic DNA. The nucleotide sequences are 99.7% identical, and the predicted processed proteins are 100% identical. Topology...

... biosynthesis glycoprotein membrane protein processing topology Biochem. J. (1999) 339, 269 279 (Printed in Great Britain) 269 Transmembrane folding of the human erythrocyte anion exchanger (AE1, Band 3) determined by scanning and insertional N-glycosylation mutagenesis Milka POPOV, Jing LI and Reinhart...