... the mechanism of this perturbation, we measured the effects of Rab14 knockdown on the trafficking kinetics of Glut4 relative to two proteins that partially co-localize with Glut4, the transferrin (Tf) receptor and low-density-lipoprotein-receptor-related protein 1 (LRP1). Our data support the hypothesis...

...Giada Sebastiani; Kostas Pantopoulos Non-transferrin-bound iron (NTBI) emerges in plasma of patients with systemic iron overload, but has also been documented in further pathological conditions. Quantification of NTBI can be useful for diagnosis and management of these disorders. However, currently...

... chaperone divalent metal transporter 1 (DMT1) ferroportin 1 (FPN1) iron transport poly(rC)-binding protein (PCBP) transferrin Iron (Fe) is an essential transition metal for life. The redox characteristics of Fe that ensure its utility as a widely employed protein co-factor also pose major...

... with protein amino groups, known as glycation, has been shown to compromise the action of Tf (transferrin), the systemic iron transporter. In order to understand the structural alterations that impair its function, Tf was glycated in vitro and the modification sites were determined by MS. Iron binding...

... an unbiased proteomics screen where we identified TfR1 (transferrin receptor 1). TfR1 is required for transferrin binding and internalization and ultimately for iron homoeostasis. TfR1 depletion does not lead to changes in IKK subunit protein levels; however, it does reduce the formation of the IKK complex...

...Leslie P. Silva; Rong-hua Yu; Charles Calmettes; Xue Yang; Trevor F. Moraes; David C. Schriemer; Anthony B. Schryvers The ability to acquire iron directly from host Tf (transferrin) is an adaptation common to important bacterial pathogens belonging to the Pasteurellaceae, Moraxellaceae...

...Maya Shvartsman; Eitan Fibach; Z. Ioav Cabantchik In the present study we analysed the mechanism of intracellular routing of iron acquired by erythroid cells via receptor-mediated endocytosis of Tf-Fe [Tf (transferrin)-iron]. Using real-time fluorimetry and flow cytometry, in conjunction...

... the virulence of a pleomorphic strain of the parasite, for reasons that are not clear. In the present paper, we report that GPI-PLC stimulates endocytosis of transferrin by 300–500%. Surprisingly, GPI-PLC is not detected at endosomes, suggesting that the enzyme does not interact directly with the endosomal...

... in the concentration of the Fe–HPO complex. Having established that the shuttling of iron from the bidentate chelator to DFO does occur under clinically relevant concentrations of chelator, studies were undertaken to evaluate whether this mechanism of transfer would apply to iron removal from transferrin. Again...

...Ali G. Khan; Stephen R. Shouldice; Shane D. Kirby; Rong-hua Yu; Leslie W. Tari; Anthony B. Schryvers The periplasmic iron-binding protein, FbpA (ferric-ion-binding protein A), performs an essential role in iron acquisition from transferrin in Haemophilus influenzae . A series of site-directed...

...Ali G. Khan; Stephen R. Shouldice; Leslie W. Tari; Anthony B. Schryvers The acquisition of iron from transferrin by Gram-negative bacterial pathogens is dependent on a periplasmic ferric-ion-binding protein, FbpA. FbpA shuttles iron from the outer membrane to an inner membrane transport complex...

...Daniel Wüstner Transport of the recycling marker transferrin was analysed in polarized hepatic HepG2 cells using quantitative fluorescence microscopy and mathematical modelling. A detailed map and kinetic model for transport of transferrin in hepatic cells was developed. Fluorescent transferrin...

... correspondence should be addressed (email [email protected] ). 15 12 2004 11 4 2005 28 4 2005 28 4 2005 The Biochemical Society, London 2005 cholesterol efflux cholesterol homoeostasis cholesterol metabolism endocytosis oxysterol-binding protein (OSBP) transferrin...

...Alessia CALZOLARI; Silvia DEAGLIO; Nadia Maria SPOSI; Eleonora PETRUCCI; Ornella MORSILLI; Marco GABBIANELLI; Fabio MALAVASI; Cesare PESCHLE; Ugo TESTA Human TFR2 (transferrin receptor 2) is a membrane-bound protein homologous with TFR1. High levels of TFR2 mRNA were found mainly in the liver...

... mice, following phagocytosis of 59 Fe-labelled transferrin—anti-transferrin immune complexes, showed reduced iron release compared with cells from wild-type iNOS littermates. Uptake of the complexes by macrophages was similar in iNOS-deficient and wild-type mice. Ferritin was up-regulated by IFNγ/LPS...

...Philippa MILLS; Kevin MILLS; Peter CLAYTON; Andrew JOHNSON; David WHITEHOUSE; Bryan WINCHESTER The N-linked glycans on transferrin and α 1 -antitrypsin from patients with congenital disorders of glycosylation type I have increased fucosylation and branching relative to normal controls. The elevated...

...James MWANJEWE; Betsy King HUI; Michael D. COUGHLIN; Ashok K. GROVER Phaeochromocytoma PC12 cells treated with nerve growth factor (NGF) differentiate into a neuronal phenotype. Here we compare the uptake of transferrin-bound and non-transferrin-bound iron in NGF-treated (neuronal phenotype...