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Articles

The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Inmaculada Segura, Angelika Harbauer

Biochem J (2024) 481 (3): 119–139.

https://doi.org/10.1042/BCJ20230110

Published: 29 January 2024

... occur in a post-translational manner [ 8 , 9 ]. This is contrasted with early findings that detected ribosomes attached to the mitochondrial surface [ 10 ], which suggested that even though mitochondrial protein import in vitro can occur post-translationally, the cell might also use localized...

View articletitled, The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Articles

Chloroplast translation factor EF-Tu of Arabidopsis thaliana can be inactivated via oxidation of a specific cysteine residue

Machi Toriu, Momoka Horie, Yuka Kumaki, Taku Yoneyama, Shin Kore-eda, Susumu Mitsuyama, Keisuke Yoshida, Toru Hisabori, Yoshitaka Nishiyama

Biochem J (2023) 480 (5): 307–318.

https://doi.org/10.1042/BCJ20220609

Published: 09 March 2023

...Machi Toriu; Momoka Horie; Yuka Kumaki; Taku Yoneyama; Shin Kore-eda; Susumu Mitsuyama; Keisuke Yoshida; Toru Hisabori; Yoshitaka Nishiyama Translational elongation factor EF-Tu, which delivers aminoacyl-tRNA to the ribosome, is susceptible to inactivation by reactive oxygen species (ROS...

View articletitled, Chloroplast translation factor EF-Tu of Arabidopsis thaliana can be inactivated via oxidation of a specific cysteine residue

Includes: Supplementary data

Articles

The role of eIF2 phosphorylation in cell and organismal physiology: new roles for well-known actors

Xuemin Wang, Christopher G. Proud

Biochem J (2022) 479 (10): 1059–1082.

https://doi.org/10.1042/BCJ20220068

Published: 23 May 2022

...Xuemin Wang; Christopher G. Proud Control of protein synthesis (mRNA translation) plays key roles in shaping the proteome and in many physiological, including homeostatic, responses. One long-known translational control mechanism involves phosphorylation of initiation factor, eIF2, which...

View articletitled, The role of eIF2 phosphorylation in cell and organismal physiology: new roles for well-known actors

Articles

Translational regulation in pathogenic and beneficial plant–microbe interactions

Soledad Traubenik, Milagros Ferrari, Flavio Antonio Blanco, María Eugenia Zanetti

Biochem J (2021) 478 (14): 2775–2788.

https://doi.org/10.1042/BCJ20210066

Published: 23 July 2021

... Society 2021 These studies highlighted the importance of the translational level of control of gene expression for plant immunity. Genes translationally regulated during PTI and ETI belongs to different functional categories, suggesting a certain degree of specificity in the translational response...

View articletitled, Translational regulation in pathogenic and beneficial plant–microbe interactions

Articles

Elongation factor eEF2 kinase and autophagy jointly promote survival of cancer cells

Roman V. Lenchine, Sushma R. Rao, Xuemin Wang, Danielle Meiwen Fang, Christopher G. Proud

Biochem J (2021) 478 (8): 1547–1569.

https://doi.org/10.1042/BCJ20210126

Published: 21 April 2021

... of the Biochemical Society 2021 alpha kinase apoptosis cancer therapy protein synthesis translation MDA-MB-231 cells were seeded at a density of 2.5 × 10 4 cells per well in a 96-well black walled opaque plates with a clear bottom and left overnight in the incubator. Once the cells had reached 90...

View articletitled, Elongation factor eEF2 kinase and autophagy jointly promote survival of cancer cells

Includes: Supplementary data

Articles

eEF2K enhances expression of PD-L1 by promoting the translation of its mRNA

Yu Wu, Jianling Xie, Xin Jin, Roman V. Lenchine, Xuemin Wang, Danielle M. Fang, Zeyad D. Nassar, Lisa M. Butler, Jing Li, Christopher G. Proud

Biochem J (2020) 477 (22): 4367–4381.

https://doi.org/10.1042/BCJ20200697

Published: 26 November 2020

... cells to escape from immunosurveillance. Ablation of eEF2K in prostate and lung cancer cells markedly reduced the expression levels of the PD-L1 protein. We show that eEF2K promotes the association of PD-L1 mRNAs with translationally active polyribosomes and that translation of the PD-L1 mRNA...

View articletitled, eEF2K enhances expression of PD-L1 by promoting the translation of its mRNA

Includes: Supplementary data

Articles

Functional characterization of a special dicistronic transcription unit encoding histone methyltransferase su(var)3-9 and translation regulator eIF2γ in Tribolium castaneum

Xiaowen Song, Qisheng Zhong, Guifang Peng, Yanhao Ji, Yuemei Zhang, Jing Tang, Jia Xie, Jingxiu Bi, Fan Feng, Bin Li

Biochem J (2020) 477 (16): 3059–3074.

https://doi.org/10.1042/BCJ20200444

Published: 28 August 2020

..., the suppressor of position-effect variegation s u(var)3-9 and the gamma subunit of eukaryotic translation initiation factor 2 ( eIF2γ ) has been found in insecta, and its significance is not well understood. Here, we analyzed the evolutionary history of this transcription unit in arthropods and its functions...

View articletitled, Functional characterization of a special dicistronic transcription unit encoding histone methyltransferase su(var)3-9 and translation regulator eIF2γ in Tribolium castaneum

Includes: Supplementary data

Articles

Mitochondrial control of cellular protein homeostasis

Karthik Mohanraj, Urszula Nowicka, Agnieszka Chacinska

Biochem J (2020) 477 (16): 3033–3054.

https://doi.org/10.1042/BCJ20190654

Published: 26 August 2020

... leads to the accumulation of mitochondrial precursor proteins in the cytosol and activates several stress response pathways. These defense mechanisms engage a network of processes involving transcription, translation, and protein clearance to restore cellular protein homeostasis. In this review, we...

View articletitled, Mitochondrial control of cellular protein homeostasis

Articles

Polyamines stimulate the CHSY1 synthesis through the unfolding of the RNA G-quadruplex at the 5′-untraslated region

Katsutoshi Yamaguchi, Kiryu Asakura, Masataka Imamura, Gota Kawai, Taiichi Sakamoto, Tomomi Furihata, Robert J. Linhardt, Kazuei Igarashi, Toshihiko Toida, Kyohei Higashi

Biochem J (2018) 475 (23): 3797–3812.

https://doi.org/10.1042/BCJ20180672

Published: 06 December 2018

... in polyamine-depleted cells. In addition, decreased levels of chondroitin synthase 1 (CHSY1) and chondroitin 4- O -sulfotransferase 2 proteins were also observed on polyamine depletion. Interestingly, the translation initiation of CHSY1 was suppressed by a highly structured sequence (positions −202 to −117...

View articletitled, Polyamines stimulate the CHSY1 synthesis through the unfolding of the RNA G-quadruplex at the 5′-untraslated region

Includes: Supplementary data

Articles

Who does TORC2 talk to?

Jianling Xie, Xuemin Wang, Christopher G. Proud

Biochem J (2018) 475 (10): 1721–1738.

https://doi.org/10.1042/BCJ20180130

Published: 24 May 2018

... ( [email protected] ) 11 2 2018 19 4 2018 23 4 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 mechanistic target of rapamycin post-translational modification translation Signalling through multiprotein...

View articletitled, Who does TORC2 talk to?

Articles

The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading

Alice C. Copsey, Simon Cooper, Robert Parker, Ella Lineham, Cuzack Lapworth, Deema Jallad, Steve Sweet, Simon J. Morley

Biochem J (2017) 474 (18): 3109–3120.

https://doi.org/10.1042/BCJ20170354

Published: 31 August 2017

...Alice C. Copsey; Simon Cooper; Robert Parker; Ella Lineham; Cuzack Lapworth; Deema Jallad; Steve Sweet; Simon J. Morley DDX3X, a helicase, can interact directly with mRNA and translation initiation factors, regulating the selective translation of mRNAs that contain a structured 5′ untranslated...

View articletitled, The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading

Includes: Supplementary data

Articles

Post-transcriptional regulation of Wnt co-receptor LRP6 and RNA-binding protein HuR by miR-29b in intestinal epithelial cells

Yanwu Li, Gang Chen, Jun-Yao Wang, Tongtong Zou, Lan Liu, Lan Xiao, Hee Kyoung Chung, Jaladanki N. Rao, Jian-Ying Wang

Biochem J (2016) 473 (11): 1641–1649.

https://doi.org/10.1042/BCJ20160057

Published: 27 May 2016

...Yanwu Li; Gang Chen; Jun-Yao Wang; Tongtong Zou; Lan Liu; Lan Xiao; Hee Kyoung Chung; Jaladanki N. Rao; Jian-Ying Wang MicroRNAs (miRNAs) control gene expression by binding to their target mRNAs for degradation and/or translation repression and are implicated in many aspects of cellular physiology...

View articletitled, Post-transcriptional regulation of Wnt co-receptor LRP6 and RNA-binding protein HuR by miR-29b in intestinal epithelial cells

Includes: Supplementary data

Articles

In vitro translation of mRNAs that are in their native ribonucleoprotein complexes

Baptiste Panthu, Fabrice Mure, Henri Gruffat, Theophile Ohlmann

Biochem J (2015) 472 (1): 111–119.

https://doi.org/10.1042/BJ20150772

Published: 30 October 2015

... journey to the cytoplasm, where it participates in translation. However, due to the size, complexity and variability of the mRNP, it remains technically challenging to assess its impact on translation. By designing a novel in vitro translational assay, we have been able to compare the translational...

View articletitled, In vitro translation of mRNAs that are in their native ribonucleoprotein complexes

Includes: Supplementary data

Articles

The MAP kinase-interacting kinases regulate cell migration, vimentin expression and eIF4E/CYFIP1 binding

James E. Beggs, Shuye Tian, Greg G. Jones, Jianling Xie, Valentina Iadevaia, Veronika Jenei, Gareth Thomas, Christopher G. Proud

Biochem J (2015) 467 (1): 63–76.

https://doi.org/10.1042/BJ20141066

Published: 20 March 2015

... for phosphorylating the translation initiation factor eIF4E. Genetic knockout of the Mnks impaired the migration of embryonic fibroblasts both in two-dimensional wound-healing experiments and in three-dimensional migration assays. Furthermore, a novel and selective Mnk inhibitor, Mnk-I1, which potently blocks eIF4E...

View articletitled, The MAP kinase-interacting kinases regulate cell migration, vimentin expression and eIF4E/CYFIP1 binding

Articles

A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

Chun Kit Kwok, Yiliang Ding, Saima Shahid, Sarah M. Assmann, Philip C. Bevilacqua

Biochem J (2015) 467 (1): 91–102.

https://doi.org/10.1042/BJ20141063

Published: 20 March 2015

... cells. However, whether GQSs form and are functional in plants remains essentially unexplored. On the basis of circular dichroism (CD)-detected titration, UV-detected melting, in-line probing (ILP) and reporter gene assay studies, we report the first example of a plant RNA GQS that inhibits translation...

View articletitled, A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

Includes: Supplementary data

Articles

p58 IPK is an inhibitor of the eIF2α kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity

Anne Roobol, Jo Roobol, Amandine Bastide, John R. P. Knight, Anne E. Willis, C. Mark Smales

Biochem J (2015) 465 (2): 213–225.

https://doi.org/10.1042/BJ20140852

Published: 06 January 2015

...Anne Roobol; Jo Roobol; Amandine Bastide; John R. P. Knight; Anne E. Willis; C. Mark Smales One of the key cellular responses to stress is the attenuation of mRNA translation and protein synthesis via the phosphorylation of eIF2α (eukaryotic translation initiation factor 2α). This is mediated...

View articletitled, p58 IPK is an inhibitor of the eIF2α kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity

Includes: Supplementary data

Articles

Archaeal MBF1 binds to 30S and 70S ribosomes via its helix–turn–helix domain

Fabian Blombach, Helene Launay, Ambrosius P. L. Snijders, Violeta Zorraquino, Hao Wu, Bart de Koning, Stan J. J. Brouns, Thijs J. G. Ettema, Carlo Camilloni, Andrea Cavalli, Michele Vendruscolo, Mark J. Dickman, Lisa D. Cabrita, Anna La Teana, Dario Benelli, Paola Londei, John Christodoulou, John van der Oost

Biochem J (2014) 462 (2): 373–384.

https://doi.org/10.1042/BJ20131474

Published: 07 August 2014

... this, we have conducted a molecular characterization of this aMBF1. Affinity purification of interacting proteins indicates that aMBF1 binds to ribosomal subunits. On sucrose density gradients, aMBF1 co-fractionates with free 30S ribosomal subunits as well as with 70S ribosomes engaged in translation...

View articletitled, Archaeal MBF1 binds to 30S and 70S ribosomes via its helix–turn–helix domain

Includes: Supplementary data

Articles

Genome-wide profiling reveals a role for T-cell intracellular antigens TIA1 and TIAR in the control of translational specificity in HeLa cells

Isabel Carrascoso, Carmen Sánchez-Jiménez, José M. Izquierdo

Biochem J (2014) 461 (1): 43–50.

https://doi.org/10.1042/BJ20140227

Published: 13 June 2014

...Isabel Carrascoso; Carmen Sánchez-Jiménez; José M. Izquierdo TIA (T-cell intracellular antigens)-knockdown HeLa cells show an increase in ribosomes and translational machinery components. This increase correlates with specific changes in translationally up-regulated mRNAs involved in cell-cycle...

View articletitled, Genome-wide profiling reveals a role for T-cell intracellular antigens TIA1 and TIAR in the control of translational specificity in HeLa cells

Articles

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h

Anne Roobol, Jo Roobol, Martin J. Carden, Matthew E. Smith, John W. B. Hershey, Amandine Bastide, John R. P. Knight, Anne E. Willis, C. Mark Smales

Biochem J (2014) 458 (2): 213–224.

https://doi.org/10.1042/BJ20130979

Published: 14 February 2014

... in translation rather than luciferase folding because, although heat- or chemically denatured firefly luciferase has been used as a model substrate for CCT, it has also been shown that de novo luciferase can fold co-translationally without the assistance of CCT [ 47 ]. The data in Supplementary Figure S11...

View articletitled, The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h

Includes: Supplementary data

Articles

Cap-binding complex (CBC)

Thomas Gonatopoulos-Pournatzis, Victoria H. Cowling

Biochem J (2014) 457 (2): 231–242.

https://doi.org/10.1042/BJ20131214

Published: 20 December 2013

... functions is CBC (cap-binding complex). CBC has a key role in several gene expression mechanisms, including transcription, splicing, transcript export and translation. Gene expression can be regulated by signalling pathways which influence CBC function. The aim of the present review is to discuss...

View articletitled, Cap-binding complex (CBC)

Articles

Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites

Michael Aregger, Victoria H. Cowling

Biochem J (2013) 455 (1): 67–73.

https://doi.org/10.1042/BJ20130378

Published: 13 September 2013

...Michael Aregger; Victoria H. Cowling Gene expression in eukaryotes is dependent on the mRNA methyl cap which mediates mRNA processing and translation initiation. Synthesis of the methyl cap initiates with the addition of 7-methylguanosine to the initiating nucleotide of RNA pol II (polymerase II...

View articletitled, Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites

Articles

Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity

Kate M. Dudek, Laura Suter, Veerle M. Darras, Emma L. Marczylo, Timothy W. Gant

Biochem J (2013) 453 (1): 71–82.

https://doi.org/10.1042/BJ20130049

Published: 13 June 2013

... colon carcinogenesis Cancer Prev. Res. 2009 2 984 994 7 Kudo K. Xi Y. Wang Y. Song B. Chu E. Ju J. Russo J. J. Translational control analysis by translationally active RNA capture/microarray analysis (TrIP-Chip) Nucleic Acids Res. 2010 38 e104 8...

View articletitled, Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity

Includes: Supplementary data

Articles

mRNA encoding WAVE–Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration

Mark Willett, Michele Brocard, Hilary J. Pollard, Simon J. Morley

Biochem J (2013) 452 (1): 45–55.

https://doi.org/10.1042/BJ20121803

Published: 25 April 2013

...] complex are localized to the leading edge of the cell and associated with sites of active translation. Furthermore, we demonstrate that steady-state levels of ArpC2 and Rac1 proteins increase at the leading edge during cell spreading, suggesting that localized protein synthesis has a pivotal role...

View articletitled, mRNA encoding WAVE–Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration

Includes: Supplementary data

Articles

Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA

Shinya Matsumoto, Takeshi Uchiumi, Hiroyuki Tanamachi, Toshiro Saito, Mikako Yagi, Shinya Takazaki, Tomotake Kanki, Dongchon Kang

Biochem J (2012) 443 (2): 573–584.

https://doi.org/10.1042/BJ20111728

Published: 27 March 2012

... protein-1) is a unique multifunctional protein that can regulate gene expression in both transcriptional and translational manners [ 10 , 11 ]. In both germinal and somatic cells, YB-1 is a major component of translationally inactive mRNPs (messenger ribonucleoprotein particles) and is mainly responsible...

View articletitled, Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA

Includes: Supplementary data

Articles

Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase

Sébastien Pyr Dit Ruys, Xuemin Wang, Ewan M. Smith, Gaëtan Herinckx, Nusrat Hussain, Mark H. Rider, Didier Vertommen, Christopher G. Proud

Biochem J (2012) 442 (3): 681–692.

https://doi.org/10.1042/BJ20111530

Published: 24 February 2012

...Sébastien Pyr Dit Ruys; Xuemin Wang; Ewan M. Smith; Gaëtan Herinckx; Nusrat Hussain; Mark H. Rider; Didier Vertommen; Christopher G. Proud eEF2K [eEF2 (eukaryotic elongation factor 2) kinase] phosphorylates and inactivates the translation elongation factor eEF2. eEF2K is not a member of the main...

View articletitled, Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase

Includes: Supplementary data

Articles

Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae

L. Ashley Cowart, Jason L. Gandy, Baby Tholanikunnel, Yusuf A. Hannun

Biochem J (2010) 431 (1): 31–38.

https://doi.org/10.1042/BJ20100307

Published: 14 September 2010

...L. Ashley Cowart; Jason L. Gandy; Baby Tholanikunnel; Yusuf A. Hannun Recent work, especially in the yeast Saccharomyces cerevisiae , has demonstrated that mRNA movement from active translation to cytoplasmic granules, termed mRNA‘p-bodies’ (processing bodies), occurs in concert with the regulation...

View articletitled, Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae

Articles

Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit

Sven Dennerlein, Agata Rozanska, Mateusz Wydro, Zofia M. A. Chrzanowska-Lightowlers, Robert N. Lightowlers

Biochem J (2010) 430 (3): 551–558.

https://doi.org/10.1042/BJ20100757

Published: 27 August 2010

... (ERAL1) mitoribosome ribosome assembly rRNA translation For affinity purification either cell lysates or mitochondria from HEK-293T cells expressing FLAG-tagged derivatives were used. Cell lysates were prepared in lysis buffer [50 mM Tris/HCl, pH 7.4, 150 mM NaCl, 10 mM MgCl 2 , 1 mM EDTA, 1...

View articletitled, Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit

Includes: Supplementary data

Articles

Selenium controls the sex-specific immune response and selenoprotein expression during the acute-phase response in mice

Mette Stoedter, Kostja Renko, Antonia Hög, Lutz Schomburg

Biochem J (2010) 429 (1): 43–51.

https://doi.org/10.1042/BJ20091868

Published: 14 June 2010

...) sexual dimorphism translation Se is an essential trace element needed for the biosynthesis of a small number of mammalian selenoproteins [ 1 ]. Se intake and personal Se status are implicated in widespread human pathologies including cancer, cardiovascular disease and neurodegeneration [ 2...

View articletitled, Selenium controls the sex-specific immune response and selenoprotein expression during the acute-phase response in mice

Articles

Selective inhibition of β-F1-ATPase mRNA translation in human tumours

Imke M. Willers, Antonio Isidoro, Álvaro D. Ortega, Pedro L. Fernández, José M. Cuezva

Biochem J (2010) 426 (3): 319–326.

https://doi.org/10.1042/BJ20091570

Published: 24 February 2010

... and squamous carcinomas of the lung. Despite the down-regulation of the protein, tumour β-mRNA levels remained either unchanged (breast and lung adenocarcinomas) or significantly increased (colon and squamous lung carcinomas) when compared with paired normal tissues, suggesting a specific translation-masking...

View articletitled, Selective inhibition of β-F1-ATPase mRNA translation in human tumours

Includes: Supplementary data

Articles

Regulation of mRNA cap methylation

Victoria H. Cowling

Biochem J (2010) 425 (2): 295–302.

https://doi.org/10.1042/BJ20091352

Published: 23 December 2009

...Victoria H. Cowling The 7-methylguanosine cap added to the 5′ end of mRNA is essential for efficient gene expression and cell viability. Methylation of the guanosine cap is necessary for the translation of most cellular mRNAs in all eukaryotic organisms in which it has been investigated. In some...

View articletitled, Regulation of mRNA cap methylation

Articles

Cytoplasmic polyadenylation-element-binding protein (CPEB)1 and 2 bind to the HIF-1 α mRNA 3′-UTR and modulate HIF-1α protein expression

Sonja Hägele, Uwe Kühn, Melanie Böning, Dörthe M. Katschinski

Biochem J (2009) 417 (1): 235–246.

https://doi.org/10.1042/BJ20081353

Published: 12 December 2008

.... Activating transcription factor 4 is translationally regulated by hypoxic stress Mol. Cell. Biol. 2004 24 7469 7482 14 Liu L. Cash T. P. Jones R. G. Keith B. Thompson C. B. Simon M. C. Hypoxia-induced energy stress regulates mRNA translation and cell growth Mol. Cell...

View articletitled, Cytoplasmic polyadenylation-element-binding protein (CPEB)1 and 2 bind to the HIF-1 α mRNA 3′-UTR and modulate HIF-1α protein expression

Articles

Regulation of gene expression by hypoxia

Niall Steven Kenneth, Sonia Rocha

Biochem J (2008) 414 (1): 19–29.

https://doi.org/10.1042/BJ20081055

Published: 29 July 2008

... integrate with HIF and how other cellular pathways such as chromatin remodelling, translation regulation and microRNA induction, contribute to the co-ordinated cellular response observed following hypoxic stress. 1 To whom correspondence should be addressed (email [email protected] ). 29 5...

View articletitled, Regulation of gene expression by hypoxia

Includes: Multimedia, Supplementary data

Articles

A decrease in cellular energy status stimulates PERK-dependent eIF2α phosphorylation and regulates protein synthesis in pancreatic β-cells

Edith Gomez, Mike L. Powell, Alan Bevington, Terence P. Herbert

Biochem J (2008) 410 (3): 485–493.

https://doi.org/10.1042/BJ20071367

Published: 27 February 2008

... of the translational ternary complex, made up of the tRNA i Met (methionyl initiator tRNA) attached to eIF2 (eukaryotic initiation factor 2) in its GTP-bound state (eIF2–GTP:tRNA i Met ) (for a review, see [ 9 ]). This ternary complex binds to the 40S ribosomal subunit, which, in association with additional...

View articletitled, A decrease in cellular energy status stimulates PERK-dependent eIF2α phosphorylation and regulates protein synthesis in pancreatic β-cells

Articles

The translation initiation factor eIF2β is an interactor of protein phosphatase-1

Paulina Wakula, Monique Beullens, Aleyde van Eynde, Hugo Ceulemans, Willy Stalmans, Mathieu Bollen

Biochem J (2006) 400 (2): 377–383.

https://doi.org/10.1042/BJ20060758

Published: 14 November 2006

...Paulina Wakula; Monique Beullens; Aleyde van Eynde; Hugo Ceulemans; Willy Stalmans; Mathieu Bollen It is reasonably well understood how the initiation of translation is controlled by reversible phosphorylation of the eukaryotic translation initiation factors eIF2α, eIF2Bϵ and eIF4E. Other...

View articletitled, The translation initiation factor eIF2β is an interactor of protein phosphatase-1

Includes: Supplementary data

Articles

The differential catalytic activity of ribosome-inactivating proteins saporin 5 and 6 is due to a single substitution at position 162

Paroma Ghosh, Janendra K. Batra

Biochem J (2006) 400 (1): 99–104.

https://doi.org/10.1042/BJ20060895

Published: 27 October 2006

... (results not shown). 1 To whom correspondence should be addressed (email [email protected] ). catalytic activity ribosome-inactivating protein (RIP) ricin saporin 5 toxin translation A functional comparison of two saporin seed isoforms, saporin 5 and saporin 6 showed saporin 6...

View articletitled, The differential catalytic activity of ribosome-inactivating proteins saporin 5 and 6 is due to a single substitution at position 162

Articles

A protein extension to shorten RNA: elongated elongation factor-Tu recognizes the D-arm of T-armless tRNAs in nematode mitochondria

Masayuki Sakurai, Yoh-ichi Watanabe, Kimitsuna Watanabe, Takashi Ohtsuki

Biochem J (2006) 399 (2): 249–256.

https://doi.org/10.1042/BJ20060781

Published: 27 September 2006

... mitochondrial translation system employs two different EF-Tu factors named EF-Tu1 and EF-Tu2. Our previous study showed that nematode Caenorhabditis elegans EF-Tu1 binds specifically to T-armless tRNA. C. elegans EF-Tu1 has a 57-amino acid C-terminal extension that is absent from canonical EF-Tu, and the T-arm...

View articletitled, A protein extension to shorten RNA: elongated elongation factor-Tu recognizes the D-arm of T-armless tRNAs in nematode mitochondria

Includes: Supplementary data

Articles

HIV protease cleaves poly(A)-binding protein

Enrique Álvarez, Alfredo Castelló, Luis Menéndez-Arias, Luis Carrasco

Biochem J (2006) 396 (2): 219–226.

https://doi.org/10.1042/BJ20060108

Published: 15 May 2006

...Enrique Álvarez; Alfredo Castelló; Luis Menéndez-Arias; Luis Carrasco The PABP [poly(A)-binding protein] is able to interact with the 3′ poly(A) tail of eukaryotic mRNA, promoting its translation. Cleavage of PABP by viral proteases encoded by several picornaviruses and caliciviruses plays a role...

View articletitled, HIV protease cleaves poly(A)-binding protein

Articles

Genomic organization of human arylamine N -acetyltransferase Type I reveals alternative promoters that generate different 5′-UTR splice variants with altered translational activities

Neville J. BUTCHER, Ajanthy ARULPRAGASAM, Hui Li GOH, Tamara DAVEY, Rodney F. MINCHIN

Biochem J (2005) 387 (1): 119–127.

https://doi.org/10.1042/BJ20040903

Published: 22 March 2005

...) promoter splice variant translation 5′ untranslated region (5′-UTR) Human NAT1 (arylamine N -acetyltransferase Type I; EC 2.3.1.5) is found in most tissues, where it catalyses the transfer of an acetyl group from acetyl-CoA to arylamine and hydrazine substrates [ 1 – 3 ], including the folate...

View articletitled, Genomic organization of human arylamine N -acetyltransferase Type I reveals alternative promoters that generate different 5′-UTR splice variants with altered translational activities

Includes: Supplementary data

Articles

Characterization of Staufen 1 ribonucleoprotein complexes

Cornelia BRENDEL, Monika REHBEIN, Hans-Jürgen KREIENKAMP, Friedrich BUCK, Dietmar RICHTER, Stefan KINDLER

Biochem J (2004) 384 (2): 239–246.

https://doi.org/10.1042/BJ20040812

Published: 23 November 2004

... concentrated in the nucleus, it also associates with translationally quiescent mRNP complexes in the cytoplasm [ 32 ]. Thus Stau1, NFAR, nucleolin, FMRP and PABP may assemble into one complex regulating mRNA trafficking and translation. RNA helicase A (RHA), another RNA-binding protein identified in Stau1 RNPs...

View articletitled, Characterization of Staufen 1 ribonucleoprotein complexes

Articles

Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes

Sonja BAUM, Margarethe BITTINS, Steffen FREY, Matthias SEEDORF

Biochem J (2004) 380 (3): 823–830.

https://doi.org/10.1042/bj20031962

Published: 15 June 2004

... a mechanism for translational regulation of these mRNAs. However, future experiments are required to ident- ify mRNAs that are translationally regulated by Scp160p. The intracellular distribution of Asc1p changes in cells entering stationary phase. In these cells, a significant amount of Asc1p was found...

View articletitled, Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes

Articles

Parasite-specific eIF2 (eukaryotic initiation factor-2) kinase required for stress-induced translation control

William J. SULLIVAN, Jr, Jana NARASIMHAN, Micah M. BHATTI, Ronald C. WEK

Biochem J (2004) 380 (2): 523–531.

https://doi.org/10.1042/bj20040262

Published: 01 June 2004

... kinase family, TgIF2K-A has an extended N-terminal region that is highly divergent from other eIF2 kinases. TgIF2K-A specifically phosphorylates the regulatory serine residue of yeast eIF2α in vitro and in vivo , and can modulate translation when expressed in the yeast model system. We also demonstrate...

View articletitled, Parasite-specific eIF2 (eukaryotic initiation factor-2) kinase required for stress-induced translation control

Articles

Regulation of protein synthesis by eIF4E phosphorylation in adult cardiocytes: the consequence of secondary structure in the 5′-untranslated region of mRNA

William J. TUXWORTH, Jr, Atif N. SAGHIR, Laura S. SPRUILL, Donald R. MENICK, Paul J. McDERMOTT

Biochem J (2004) 378 (1): 73–82.

https://doi.org/10.1042/bj20031027

Published: 15 February 2004

... that increases in eIF4E activity selectively improve the translational efficiency of mRNAs that have an excessive amount of secondary structure in the 5´-UTR (5´-untranslated region). The activity of eIF4E was modified in primary cultures of adult cardiocytes using adenoviral gene transfer to increase either...

View articletitled, Regulation of protein synthesis by eIF4E phosphorylation in adult cardiocytes: the consequence of secondary structure in the 5′-untranslated region of mRNA

Articles

Molecular identification of the long isoform of the human neuropeptide Y Y5 receptor and pharmacological comparison with the short Y5 receptor isoform

Marianne RODRIGUEZ, Valérie AUDINOT, Sandra DROMAINT, Christelle MACIA, Véronique LAMAMY, Philippe BEAUVERGER, Hervé RIQUE, Jérôme IMBERT, Jean Paul NICOLAS, Jean A. BOUTIN, Jean Pierre GALIZZI

Biochem J (2003) 369 (3): 667–673.

https://doi.org/10.1042/bj20020739

Published: 01 February 2003

... of this transcript was confirmed by isolating part of its 5′ untranslated region through 5′ rapid amplification of cDNA ends and analysing its tissue distribution. To study the initiation of translation on Y5 L mRNA, we cloned the Y5 L cDNA and two Y5 L cDNA mutants lacking the first or the second putative...

View articletitled, Molecular identification of the long isoform of the human neuropeptide Y Y5 receptor and pharmacological comparison with the short Y5 receptor isoform

Articles

Effects of osmolarity, ions and compatible osmolytes on cell-free protein synthesis

Maurizio BRIGOTTI, Pier Giorgio PETRONINI, Domenica CARNICELLI, Roberta R. ALFIERI, Mara A. BONELLI, Angelo F. BORGHETTI, Kenneth P. WHEELER

Biochem J (2003) 369 (2): 369–374.

https://doi.org/10.1042/bj20021056

Published: 15 January 2003

... examined. Translation of globin mRNA by rabbit reticulocyte lysate decreased by 30—60% when osmolality was increased from 0.35 to 0.53osmol/kg of water by the addition of NaCl, KCl, CH 3 CO 2 Na or CH 3 CO 2 K. In contrast, equivalent additions of the compatible osmolytes betaine or myo -inositol caused...

View articletitled, Effects of osmolarity, ions and compatible osmolytes on cell-free protein synthesis

Articles

Genomic organization of the mouse peroxisome proliferator-activated receptor β/δ gene: alternative promoter usage and splicing yield transcripts exhibiting differential translational efficiency

Leif K. LARSEN, Ez-Zoubir AMRI, Susanne MANDRUP, Corinne PACOT, Karsten KRISTIANSEN

Biochem J (2002) 366 (3): 767–775.

https://doi.org/10.1042/bj20011821

Published: 15 September 2002

... rise to a transcript with a 548nt 5′-untranslated leader containing eight upstream AUG codons. We show that the presence of the 548nt leader resulted in a low translational efficiency of the corresponding PPARβ/Δ mRNA and propose, based on structural features of the 5′-untranslated region...

View articletitled, Genomic organization of the mouse peroxisome proliferator-activated receptor β/δ gene: alternative promoter usage and splicing yield transcripts exhibiting differential translational efficiency

Articles

Distinct roles of activating transcription factor 6 (ATF6) and double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (PERK) in transcription during the mammalian unfolded protein response

Tetsuya OKADA, Hiderou YOSHIDA, Rieko AKAZAWA, Manabu NEGISHI, Kazutoshi MORI

Biochem J (2002) 366 (2): 585–594.

https://doi.org/10.1042/bj20020391

Published: 01 September 2002

...)-mediated signalling pathway appeared to be responsible for induction of the remaining genes, which are not involved in secretion, but may be important after cellular recovery from ER stress. In higher eukaryotes, the PERK-mediated translational-attenuation system is known to operate in concert...

View articletitled, Distinct roles of activating transcription factor 6 (ATF6) and double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (PERK) in transcription during the mammalian unfolded protein response

Articles

Assembly of the ribonucleoprotein complex containing the mRNA of the β-subunit of the mitochondrial H+-ATP synthase requires the participation of two distal cis-acting elements and a complex set of cellular trans-acting proteins

Javier RICART, José M. IZQUIERDO, Carlo M. Di LIEGRO, José M. CUEZVA

Biochem J (2002) 365 (2): 417–428.

https://doi.org/10.1042/bj20011726

Published: 15 July 2002

... element is the 3′ non-translated region of the mRNA. Complex sets of proteins from the soluble and non-soluble fractions of the liver interact with the β1.2 and 3′β elements. A soluble p88, present also in reticulocyte lysate, displays binding specificity for both the cis -acting elements. Sedimentation...

View articletitled, Assembly of the ribonucleoprotein complex containing the mRNA of the β-subunit of the mitochondrial H+-ATP synthase requires the participation of two distal cis-acting elements and a complex set of cellular trans-acting proteins

Articles

Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates

Atif N. SAGHIR, William J. TUXWORTH, Jr, Curt H. HAGEDORN, Paul J. McDERMOTT

Biochem J (2001) 356 (2): 557–566.

https://doi.org/10.1042/bj3560557

Published: 24 May 2001

... a net accumulation of constitutively expressed proteins in the cardio- cyte such as myofibrillar and mitochondrial proteins [4,5]. There are two general translation mechanisms that accelerate the rate of protein synthesis, efficiency and capacity. Efficiency is defined as the utilization of the mRNA...

View articletitled, Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates

Articles

Phosphorylation of elongation factor-2 kinase on serine 499 by cAMP-dependent protein kinase induces Ca2+/calmodulin-independent activity

Tricia A. DIGGLE, Tatiana SUBKHANKULOVA, Kathryn S. LILLEY, Nita SHIKOTRA, Anne E. WILLIS, Nicholas T. REDPATH

Biochem J (2001) 353 (3): 621–626.

https://doi.org/10.1042/bj3530621

Published: 25 January 2001

...Tricia A. DIGGLE; Tatiana SUBKHANKULOVA; Kathryn S. LILLEY; Nita SHIKOTRA; Anne E. WILLIS; Nicholas T. REDPATH Elongation factor-2 kinase (eEF-2K) negatively regulates mRNA translation via the phosphorylation and inactivation of elongation factor-2 (eEF-2). We have shown previously that purified...

View articletitled, Phosphorylation of elongation factor-2 kinase on serine 499 by cAMP-dependent protein kinase induces Ca2+/calmodulin-independent activity

Articles

Internal-ribosome-entry-site functional activity of the 3′-untranslated region of the mRNA for the β subunit of mitochondrial H+-ATP synthase

José M. IZQUIERDO, José M. CUEZVA

Biochem J (2000) 346 (3): 849–855.

https://doi.org/10.1042/bj3460849

Published: 07 March 2000

...José M. IZQUIERDO; José M. CUEZVA Translation in vitro of the mammalian nucleus-encoded mRNA for the β subunit of mitochondrial H + -ATP synthase (β-mRNA) of oxidative phosphorylation is promoted by a 150 nt translational enhancer sequence in the 3ʹ-untranslated region (3ʹ UTR). Titration...

View articletitled, Internal-ribosome-entry-site functional activity of the 3′-untranslated region of the mRNA for the β subunit of mitochondrial H+-ATP synthase

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