Page 1 | Search Results | Biochemical Journal

1-50 of 58

Sort by

Sort Order Select

Articles

Permeation mechanisms of hydrogen peroxide and water through Plasma Membrane Intrinsic Protein aquaporins

Jonathan Chevriau, Gerardo Zerbetto De Palma, Cintia Jozefkowicz, Victoria Vitali, Agustina Canessa Fortuna, Nicolas Ayub, Gabriela Soto, Gerd Patrick Bienert, Ari Zeida, Karina Alleva

Biochem J (2024) 481 (19): 1329–1347.

https://doi.org/10.1042/BCJ20240310

Published: 25 September 2024

...Jonathan Chevriau; Gerardo Zerbetto De Palma; Cintia Jozefkowicz; Victoria Vitali; Agustina Canessa Fortuna; Nicolas Ayub; Gabriela Soto; Gerd Patrick Bienert; Ari Zeida; Karina Alleva Hydrogen peroxide (H 2 O 2 ) transport by aquaporins (AQP) is a critical feature for cellular redox signaling...

View article titled, Permeation mechanisms of hydrogen peroxide and water through Plasma Membrane Intrinsic Protein aquaporins

Includes: Supplementary data

Articles

Thermostability-based binding assays reveal complex interplay of cation, substrate and lipid binding in the bacterial DASS transporter, VcINDY

Connor D. D. Sampson, Cristina Fàbregas Bellavista, Matthew J. Stewart, Christopher Mulligan

Biochem J (2021) 478 (21): 3847–3867.

https://doi.org/10.1042/BCJ20210061

Published: 09 November 2021

...Connor D. D. Sampson; Cristina Fàbregas Bellavista; Matthew J. Stewart; Christopher Mulligan The divalent anion sodium symporter (DASS) family of transporters (SLC13 family in humans) are key regulators of metabolic homeostasis, disruption of which results in protection from diabetes and obesity...

View article titled, Thermostability-based binding assays reveal complex interplay of cation, substrate and lipid binding in the bacterial DASS transporter, VcINDY

Includes: Supplementary data

Articles

All-trans retinoic acid reduces the transcriptional regulation of intestinal sodium-dependent phosphate co-transporter gene ( Npt2b )

Masashi Masuda, Hironori Yamamoto, Yuichiro Takei, Otoki Nakahashi, Yuichiro Adachi, Kohta Ohnishi, Hirokazu Ohminami, Hisami Yamanaka-Okumura, Hiroshi Sakaue, Makoto Miyazaki, Eiji Takeda, Yutaka Taketani

Biochem J (2020) 477 (4): 817–831.

https://doi.org/10.1042/BCJ20190716

Published: 28 February 2020

...-dependent co-transporter (Npt2b) and by renal reabsorption via Npt2a and Npt2c. Although we previously reported that vitamin A-deficient (VAD) rats had increased urine Pi excretion through the decreased renal expression of Npt2a and Npt2c, the effect of vitamin A on the intestinal Npt2b expression remains...

View article titled, All-trans retinoic acid reduces the transcriptional regulation of intestinal sodium-dependent phosphate co-transporter gene ( Npt2b )

Includes: Supplementary data

Articles

ATP-dependent thermostabilization of human P-glycoprotein (ABCB1) is blocked by modulators

Sabrina Lusvarghi, Suresh V. Ambudkar

Biochem J (2019) 476 (24): 3737–3750.

https://doi.org/10.1042/BCJ20190736

Published: 17 December 2019

...Sabrina Lusvarghi; Suresh V. Ambudkar P-glycoprotein (P-gp), an ATP-binding cassette transporter associated with multidrug resistance in cancer cells, is capable of effluxing a number of xenobiotics as well as anticancer drugs. The transport of molecules through the transmembrane (TM) region of P...

View article titled, ATP-dependent thermostabilization of human P-glycoprotein (ABCB1) is blocked by modulators

Includes: Supplementary data

Articles

Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

David P. Scanlon, Michael W. Salter

Biochem J (2019) 476 (1): 25–37.

https://doi.org/10.1042/BCJ20180473

Published: 07 January 2019

... multifunctional proteins trafficking transport Historically, mitochondria have been perceived almost exclusively as the cell's energy generator, principally responsible for cellular ATP (adenosine triphosphate) production, as well as an evolutionary curiosity due to their endosymbiotic nature and α...

View article titled, Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

Articles

Identification of residues critical for proton-coupled glutathione translocation in the yeast glutathione transporter, Hgt1p

Mohammad Zulkifli, Anand Kumar Bachhawat

Biochem J (2017) 474 (11): 1807–1821.

https://doi.org/10.1042/BCJ20161063

Published: 16 May 2017

...Mohammad Zulkifli; Anand Kumar Bachhawat The proton gradient acts as the driving force for the transport of many metabolites across fungal and plant plasma membranes. Identifying the mechanism of proton relay is critical for understanding the mechanism of transport mediated by these transporters...

View article titled, Identification of residues critical for proton-coupled glutathione translocation in the yeast glutathione transporter, Hgt1p

Includes: Supplementary data

Articles

TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex

Sarah M. Smith, Andrew Yarwood, Roland A. Fleck, Colin Robinson, Corinne J. Smith

Biochem J (2017) 474 (9): 1495–1508.

https://doi.org/10.1042/BCJ20160952

Published: 19 April 2017

...Sarah M. Smith; Andrew Yarwood; Roland A. Fleck; Colin Robinson; Corinne J. Smith The twin-arginine translocation (Tat) system is an integral membrane protein complex that accomplishes the remarkable feat of transporting large, fully folded polypeptides across the inner membrane of bacteria...

View article titled, TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex

Includes: Supplementary data

Articles

Astrocytic transporters in Alzheimer's disease

Chris Ugbode, Yuhan Hu, Benjamin Whalley, Chris Peers, Marcus Rattray, Mark L. Dallas

Biochem J (2017) 474 (3): 333–355.

https://doi.org/10.1042/BCJ20160505

Published: 20 January 2017

... transport system covers a diverse array of proteins involved in metabolic support, neurotransmission and synaptic architecture. Therefore, specific targeting of individual transporter families has the potential to suppress neurodegeneration, a characteristic hallmark of AD. A small number of the 400...

View article titled, Astrocytic transporters in Alzheimer's disease

Articles

Channel–transporter complexes: an emerging theme in cell signaling

Geoffrey W. Abbott

Biochem J (2016) 473 (21): 3759–3763.

https://doi.org/10.1042/BCJ20160685C

Published: 27 October 2016

...–transporter (‘chansporter’) complexes, which together constitute an emerging theme in cell signaling. Correspondence: Geoffrey W. Abbott ( [email protected] ) neuronal ion transport physiology potassium channels renal ion transport sodium channels transport The primary difference between true...

View article titled, Channel–transporter complexes: an emerging theme in cell signaling

Articles

Functional and mechanistic roles of the human proton-coupled folate transporter transmembrane domain 6–7 linker

Mike R. Wilson, Zhanjun Hou, Lucas J. Wilson, Jun Ye, Larry H. Matherly

Biochem J (2016) 473 (20): 3545–3562.

https://doi.org/10.1042/BCJ20160399

Published: 11 October 2016

...Mike R. Wilson; Zhanjun Hou; Lucas J. Wilson; Jun Ye; Larry H. Matherly The proton-coupled folate transporter (PCFT; SLC46A1) is a folate–proton symporter expressed in solid tumors and is used for tumor-targeted delivery of cytotoxic antifolates. Topology modeling suggests that the PCFT secondary...

View article titled, Functional and mechanistic roles of the human proton-coupled folate transporter transmembrane domain 6–7 linker

Includes: Supplementary data

Articles

The role of TREX in gene expression and disease

Catherine G. Heath, Nicolas Viphakone, Stuart A. Wilson

Biochem J (2016) 473 (19): 2911–2935.

https://doi.org/10.1042/BCJ20160010

Published: 27 September 2016

... functions with an emphasis on mammalian systems. mRNA export nucleocytoplasmic NXF1 RNA processing THO transport Correspondence: Stuart A. Wilson ( [email protected] ) 22 1 2016 11 5 2016 23 5 2016 © 2016 The Author(s) 2016 This is an open access...

View article titled, The role of TREX in gene expression and disease

Articles

The sodium chloride cotransporter (NCC) and epithelial sodium channel (ENaC) associate

Abinash C. Mistry, Brandi M. Wynne, Ling Yu, Viktor Tomilin, Qiang Yue, Yiqun Zhou, Otor Al-Khalili, Rickta Mallick, Hui Cai, Abdel A. Alli, Benjamin Ko, Alexa Mattheyses, Hui-Fang Bao, Oleh Pochynyuk, Franziska Theilig, Douglas C. Eaton, Robert S. Hoover

Biochem J (2016) 473 (19): 3237–3252.

https://doi.org/10.1042/BCJ20160312

Published: 27 September 2016

... cotransporter (NCC) and the epithelial sodium channel (ENaC) are two of the most important determinants of salt balance and thus systemic blood pressure. Abnormalities in either result in profound changes in blood pressure. There is one segment of the nephron where these two sodium transporters are coexpressed...

View article titled, The sodium chloride cotransporter (NCC) and epithelial sodium channel (ENaC) associate

Articles

Role of N-glycosylation in renal betaine transport

Eva S. Schweikhard, Birgitta C. Burckhardt, Friedericke Joos, Cristina Fenollar-Ferrer, Lucy R. Forrest, Stephen A. Kempson, Christine Ziegler

Biochem J (2015) 470 (2): 169–179.

https://doi.org/10.1042/BJ20131031

Published: 20 August 2015

...Eva S. Schweikhard; Birgitta C. Burckhardt; Friedericke Joos; Cristina Fenollar-Ferrer; Lucy R. Forrest; Stephen A. Kempson; Christine Ziegler The osmolyte and folding chaperone betaine is transported by the renal Na + -coupled GABA (γ-aminobutyric acid) symporter BGT-1 (betaine/GABA transporter 1...

View article titled, Role of N-glycosylation in renal betaine transport

Articles

Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species

Chatchawal Phansopa, Sumita Roy, John B. Rafferty, C. W. Ian Douglas, Jagroop Pandhal, Phillip C. Wright, David J. Kelly, Graham P. Stafford

Biochem J (2014) 458 (3): 499–511.

https://doi.org/10.1042/BJ20131415

Published: 28 February 2014

... forsythia that includes a novel outer membrane sialic acid-transport system (NanOU), where NanO (neuraminate outer membrane permease) is a putative TonB-dependent receptor and NanU (extracellular neuraminate uptake protein) is a predicted SusD family protein. Using heterologous complementation of nanOU...

View article titled, Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species

Includes: Supplementary data

Articles

Physiological and pathological roles of mitochondrial SLC25 carriers

Manuel Gutiérrez-Aguilar, Christopher P. Baines

Biochem J (2013) 454 (3): 371–386.

https://doi.org/10.1042/BJ20121753

Published: 29 August 2013

... mitochondrial membrane. Among these carriers, the six-transmembrane-helix mitochondrial SLC25 (solute carrier family 25) proteins facilitate transport of solutes with disparate chemical identities across the inner mitochondrial membrane. Although their proper function replenishes building blocks needed...

View article titled, Physiological and pathological roles of mitochondrial SLC25 carriers

Articles

Structural model of a putrescine-cadaverine permease from Trypanosoma cruzi predicts residues vital for transport and ligand binding

Radika Soysa, Hanka Venselaar, Jacqueline Poston, Buddy Ullman, Marie-Pierre Hasne

Biochem J (2013) 452 (3): 423–432.

https://doi.org/10.1042/BJ20130350

Published: 31 May 2013

...Radika Soysa; Hanka Venselaar; Jacqueline Poston; Buddy Ullman; Marie-Pierre Hasne The TcPOT1.1 gene from Trypanosoma cruzi encodes a high affinity putrescine-cadaverine transporter belonging to the APC (amino acid/polyamine/organocation) transporter superfamily. No experimental three-dimensional...

View article titled, Structural model of a putrescine-cadaverine permease from Trypanosoma cruzi predicts residues vital for transport and ligand binding

Includes: Supplementary data

Articles

Homo- and hetero-dimeric architecture of the human liver Na + -dependent taurocholate co-transporting protein

Ingrid T. G. W. Bijsmans, Rianne A. M. Bouwmeester, Joachim Geyer, Klaas Nico Faber, Stan F. J. van de Graaf

Biochem J (2012) 441 (3): 1007–1016.

https://doi.org/10.1042/BJ20111234

Published: 16 January 2012

...Ingrid T. G. W. Bijsmans; Rianne A. M. Bouwmeester; Joachim Geyer; Klaas Nico Faber; Stan F. J. van de Graaf The NTCP (Na + –taurocholate co-transporting protein)/SLC10A [solute carrier family 10 (Na + /bile acid co-transporter family)] 1 is tightly controlled to ensure hepatic bile salt uptake...

View article titled, Homo- and hetero-dimeric architecture of the human liver Na + -dependent taurocholate co-transporting protein

Includes: Supplementary data

Articles

Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli

Lorena Pochini, Mariafrancesca Scalise, Michele Galluccio, Linda Amelio, Cesare Indiveri

Biochem J (2011) 439 (2): 227–233.

https://doi.org/10.1042/BJ20110544

Published: 28 September 2011

...Lorena Pochini; Mariafrancesca Scalise; Michele Galluccio; Linda Amelio; Cesare Indiveri The hOCTN1 (human organic cation transporter 1) overexpressed in Escherichia coli and purified by Ni-chelating chromatography has been reconstituted in liposomes by detergent removal with a batch-wise procedure...

View article titled, Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli

Includes: Supplementary data

Articles

Diet-induced epigenetic regulation in vivo of the intestinal fructose transporter Glut5 during development of rat small intestine

Takuji Suzuki, Veronique Douard, Kazuki Mochizuki, Toshinao Goda, Ronaldo P. Ferraris

Biochem J (2011) 435 (1): 43–53.

https://doi.org/10.1042/BJ20101987

Published: 15 March 2011

...Takuji Suzuki; Veronique Douard; Kazuki Mochizuki; Toshinao Goda; Ronaldo P. Ferraris Metabolic complications arising from excessive fructose consumption are increasing dramatically even in young children, but little is known about ontogenetic mechanisms regulating Glut5 [glucose transporter 5...

View article titled, Diet-induced epigenetic regulation in vivo of the intestinal fructose transporter Glut5 during development of rat small intestine

Includes: Supplementary data

Articles

Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase

Aiko Sato, Yuki Sato, Yoichiro Fukao, Masayuki Fujiwara, Taishi Umezawa, Kazuo Shinozaki, Takao Hibi, Mitsutaka Taniguchi, Hiroshi Miyake, Derek B. Goto, Nobuyuki Uozumi

Biochem J (2009) 424 (3): 439–448.

https://doi.org/10.1042/BJ20091221

Published: 10 December 2009

... was examined by single point mutations and measurement of KAT1 channel activities in Xenopus oocyte and yeast systems. Modification of Thr 308 had minimal effect on KAT1 activity. On the other hand, modification of Thr 306 reduced the K + transport uptake activity of KAT1 in both systems, indicating that Thr...

View article titled, Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase

Includes: Supplementary data

Articles

The NRAMP6 metal transporter contributes to cadmium toxicity

Rémy Cailliatte, Bruno Lapeyre, Jean-François Briat, Stéphane Mari, Catherine Curie

Biochem J (2009) 422 (2): 217–228.

https://doi.org/10.1042/BJ20090655

Published: 13 August 2009

...Rémy Cailliatte; Bruno Lapeyre; Jean-François Briat; Stéphane Mari; Catherine Curie NRAMP (natural resistance-associated macrophage protein) homologues are evolutionarily conserved bivalent metal transporters. In Arabidopsis , AtNRAMP3 and AtNRAMP4 play a key role in iron nutrition...

View article titled, The NRAMP6 metal transporter contributes to cadmium toxicity

Includes: Supplementary data

Articles

Trafficking properties and activity regulation of the neuronal glycine transporter GLYT2 by protein kinase C

Amparo Fornés, Enrique Núñez, Pablo Alonso-Torres, Carmen Aragón, Beatriz López-Corcuera

Biochem J (2008) 412 (3): 495–506.

https://doi.org/10.1042/BJ20071018

Published: 28 May 2008

...Amparo Fornés; Enrique Núñez; Pablo Alonso-Torres; Carmen Aragón; Beatriz López-Corcuera The neuronal glycine transporter GLYT2 controls the availability of the neurotransmitter in glycinergic synapses, and the modulation of its function may influence synaptic transmission. The active transporter...

View article titled, Trafficking properties and activity regulation of the neuronal glycine transporter GLYT2 by protein kinase C

Articles

Molecular identification of three Arabidopsis thaliana mitochondrial dicarboxylate carrier isoforms: organ distribution, bacterial expression, reconstitution into liposomes and functional characterization

Luigi Palmieri, Nathalie Picault, Roberto Arrigoni, Evelyne Besin, Ferdinando Palmieri, Michael Hodges

Biochem J (2008) 410 (3): 621–629.

https://doi.org/10.1042/BJ20070867

Published: 27 February 2008

... belonging to the mitochondrial carrier protein family. DIC1 and DIC2 are broadly expressed at comparable levels in all the tissues investigated. DIC1–DIC3 have been reported previously as uncoupling proteins, but direct transport assays with recombinant and reconstituted DIC proteins clearly demonstrate...

View article titled, Molecular identification of three Arabidopsis thaliana mitochondrial dicarboxylate carrier isoforms: organ distribution, bacterial expression, reconstitution into liposomes and functional characterization

Articles

Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study

Samir El-Andaloussi, Peter Järver, Henrik J. Johansson, Ülo Langel

Biochem J (2007) 407 (2): 285–292.

https://doi.org/10.1042/BJ20070507

Published: 25 September 2007

... The Authors Journal compilation © 2007 Biochemical Society 2007 cell-penetrating peptide cytotoxicity delivery vector penetratin transactivator of transcription (Tat) transport The ability to cross the lipid bilayer of cells and access the cell interior is still one of the major obstacles...

View article titled, Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study

Articles

Metabolite transport across the peroxisomal membrane

Wouter F. Visser, Carlo W. T. van Roermund, Lodewijk Ijlst, Hans R. Waterham, Ronald J. A. Wanders

Biochem J (2007) 401 (2): 365–375.

https://doi.org/10.1042/BJ20061352

Published: 21 December 2006

..., especially in higher eukaryotes. Peroxisomes catalyse a number of essential metabolic functions including fatty acid β-oxidation, ether phospholipid biosynthesis, fatty acid α-oxidation and glyoxylate detoxification. The involvement of peroxisomes in these metabolic pathways necessitates the transport...

View article titled, Metabolite transport across the peroxisomal membrane

Articles

The human Vps29 retromer component is a metallo-phosphoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide

Ester Damen, Elmar Krieger, Jens E. Nielsen, Jelle Eygensteyn, Jeroen E. M. Van Leeuwen

Biochem J (2006) 398 (3): 399–409.

https://doi.org/10.1042/BJ20060033

Published: 29 August 2006

...Ester Damen; Elmar Krieger; Jens E. Nielsen; Jelle Eygensteyn; Jeroen E. M. Van Leeuwen The retromer complex is involved in the retrograde transport of the CI-M6PR (cation-independent mannose 6-phosphate receptor) from endosomes to the Golgi. It is a hetero-trimeric complex composed of Vps26...

View article titled, The human Vps29 retromer component is a metallo-phosphoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide

Includes: Supplementary data

Articles

Agmatine is transported into liver mitochondria by a specific electrophoretic mechanism

Mauro Salvi, Valentina Battaglia, Mario Mancon, Sebastiano Colombatto, Carlo Cravanzola, Rita Calheiros, Maria P. M. Marques, Maria A. Grillo, Antonio Toninello

Biochem J (2006) 396 (2): 337–345.

https://doi.org/10.1042/BJ20060003

Published: 15 May 2006

...Mauro Salvi; Valentina Battaglia; Mario Mancon; Sebastiano Colombatto; Carlo Cravanzola; Rita Calheiros; Maria P. M. Marques; Maria A. Grillo; Antonio Toninello Agmatine, a divalent diamine with two positive charges at physiological pH, is transported into the matrix of liver mitochondria...

View article titled, Agmatine is transported into liver mitochondria by a specific electrophoretic mechanism

Articles

Life cycle of connexins in health and disease

Dale W. Laird

Biochem J (2006) 394 (3): 527–543.

https://doi.org/10.1042/BJ20051922

Published: 24 February 2006

... transport through the secretory pathway, gap-junction assembly at the cell surface, internalization and degradation. 1 email [email protected] 5 12 2005 10 1 2006 The Biochemical Society, London 2006 assembly connexin gap junction transport Table 1...

View article titled, Life cycle of connexins in health and disease

Articles

Identification of a mitochondrial transporter for pyrimidine nucleotides in Saccharomyces cerevisiae : bacterial expression, reconstitution and functional characterization

Carlo Marya Thomas Marobbio, Maria Antonietta Di Noia, Ferdinando Palmieri

Biochem J (2006) 393 (2): 441–446.

https://doi.org/10.1042/BJ20051284

Published: 23 December 2005

... outside the mitochondrial matrix and must therefore be transported across the permeability barrier of the mitochondrial inner membrane. However, no protein has ever been found to be associated with this transport activity. In the present study, Rim2p has been identified as a yeast mitochondrial pyrimidine...

View article titled, Identification of a mitochondrial transporter for pyrimidine nucleotides in Saccharomyces cerevisiae : bacterial expression, reconstitution and functional characterization

Articles

Demonstration and characterization of phosphate transport in mammalian peroxisomes

Wouter F. Visser, Carlo W. van Roermund, Lodewijk Ijlst, Klaas J. Hellingwerf, Ronald J. A. Wanders, Hans R. Waterham

Biochem J (2005) 389 (3): 717–722.

https://doi.org/10.1042/BJ20041846

Published: 26 July 2005

...Wouter F. Visser; Carlo W. van Roermund; Lodewijk Ijlst; Klaas J. Hellingwerf; Ronald J. A. Wanders; Hans R. Waterham It is now well established that the peroxisomal membrane is not freely permeable to small molecules in vivo , which implies the existence of metabolite transporters...

View article titled, Demonstration and characterization of phosphate transport in mammalian peroxisomes

Articles

Molecular cloning and functional characterization of a high-affinity zinc importer ( Dr ZIP1) from zebrafish ( Danio rerio )

Andong QIU, Majid SHAYEGHI, Christer HOGSTRAND

Biochem J (2005) 388 (3): 745–754.

https://doi.org/10.1042/BJ20041807

Published: 07 June 2005

... transporter of the SLC39A family [commonly referred to as the ZIP (Zrt- and Irt-related protein) family] from the gill of zebrafish ( Danio rerio ) ( Dr ZIP1). Dr ZIP1 protein was found to localize at the plasma membrane and to function as a zinc uptake transporter when being expressed in either chinook...

View article titled, Molecular cloning and functional characterization of a high-affinity zinc importer ( Dr ZIP1) from zebrafish ( Danio rerio )

Articles

The human multidrug-resistance-associated protein MRP1 mediates ATP-dependent transport of unconjugated bilirubin

Igino RIGATO, Lorella PASCOLO, Cristina FERNETTI, J. Donald OSTROW, Claudio TIRIBELLI

Biochem J (2004) 383 (2): 335–341.

https://doi.org/10.1042/BJ20040599

Published: 08 October 2004

...Igino RIGATO; Lorella PASCOLO; Cristina FERNETTI; J. Donald OSTROW; Claudio TIRIBELLI Results of previous studies have suggested that UCB (unconjugated bilirubin) may be transported by MRP1/Mrp1 (multidrug-resistance-associated protein 1). To test this hypothesis directly, [ 3 H]UCB transport...

View article titled, The human multidrug-resistance-associated protein MRP1 mediates ATP-dependent transport of unconjugated bilirubin

Articles

Characterization of three novel members of the Arabidopsis thaliana equilibrative nucleoside transporter (ENT) family

Alexandra WORMIT, Michaela TRAUB, Martin FLÖRCHINGER, H. Ekkehard NEUHAUS, Torsten MÖHLMANN

Biochem J (2004) 383 (1): 19–26.

https://doi.org/10.1042/BJ20040389

Published: 24 September 2004

...Alexandra WORMIT; Michaela TRAUB; Martin FLÖRCHINGER; H. Ekkehard NEUHAUS; Torsten MÖHLMANN Research on metabolism of nucleotides and their derivatives has gained increasing interest in the recent past. This includes de novo synthesis, analysis of salvage pathways, breakdown and transport...

View article titled, Characterization of three novel members of the Arabidopsis thaliana equilibrative nucleoside transporter (ENT) family

Articles

Analysis of Plasmodium vivax hexose transporters and effects of a parasitocidal inhibitor

Thierry JOËT, Kesinee CHOTIVANICH, Kamolrat SILAMUT, Asha P. PATEL, Christophe MORIN, Sanjeev KRISHNA

Biochem J (2004) 381 (3): 905–909.

https://doi.org/10.1042/BJ20040433

Published: 27 July 2004

... resistance to current treatments. To study new potential targets, we have functionally characterized two natural variants of the hexose transporter of P. vivax (PvHT) after heterologous expression in Xenopus oocytes. We show that PvHT transports both glucose and fructose. Differences in the affinity...

View article titled, Analysis of Plasmodium vivax hexose transporters and effects of a parasitocidal inhibitor

Articles

Partial reconstruction of in vitro gluconeogenesis arising from mitochondrial l-lactate uptake/metabolism and oxaloacetate export via novel l-lactate translocators

Lidia de BARI, Anna ATLANTE, Daniela VALENTI, Salvatore PASSARELLA

Biochem J (2004) 380 (1): 231–242.

https://doi.org/10.1042/bj20031981

Published: 15 May 2004

... correspondence should be addressed (e-mail [email protected] ). 23 12 2003 10 2 2004 11 2 2004 11 2 2004 The Biochemical Society, London ©2004 2004 antiporter dehydrogenase glyceraldheyde-3-phosphate symporter transport Abbreviations used: AOA, aminooxyacetate; α...

View article titled, Partial reconstruction of in vitro gluconeogenesis arising from mitochondrial l-lactate uptake/metabolism and oxaloacetate export via novel l-lactate translocators

Articles

Identification of the human mitochondrial S-adenosylmethionine transporter: bacterial expression, reconstitution, functional characterization and tissue distribution

G. AGRIMI, M. A. Di NOIA, C. M. T. MAROBBIO, G. FIERMONTE, F. M. LASORSA, F. PALMIERI

Biochem J (2004) 379 (1): 183–190.

https://doi.org/10.1042/bj20031664

Published: 01 April 2004

...G. AGRIMI; M. A. Di NOIA; C. M. T. MAROBBIO; G. FIERMONTE; F. M. LASORSA; F. PALMIERI The mitochondrial carriers are a family of transport proteins that, with a few exceptions, are found in the inner membranes of mitochondria. They shuttle metabolites and cofactors through this membrane...

View article titled, Identification of the human mitochondrial S-adenosylmethionine transporter: bacterial expression, reconstitution, functional characterization and tissue distribution

Articles

Methyl-beta-cyclodextrin stimulates glucose uptake in Clone 9 cells: a possible role for lipid rafts

Kay BARNES, Jean C. INGRAM, Matthew D. M. BENNETT, Gordon W. STEWART, Stephen A. BALDWIN

Biochem J (2004) 378 (2): 343–351.

https://doi.org/10.1042/bj20031186

Published: 01 March 2004

...Kay BARNES; Jean C. INGRAM; Matthew D. M. BENNETT; Gordon W. STEWART; Stephen A. BALDWIN An acute increase in the V max for glucose uptake occurs in many mammalian cell types after exposure to osmotic or metabolic stress. In the rat epithelial Clone 9 cell line, the glucose transporter isoform...

View article titled, Methyl-beta-cyclodextrin stimulates glucose uptake in Clone 9 cells: a possible role for lipid rafts

Articles

Efficient selenium transfer from mother to offspring in selenoprotein-P-deficient mice enables dose-dependent rescue of phenotypes associated with selenium deficiency

Ulrich SCHWEIZER, Marten MICHAELIS, Josef KÖHRLE, Lutz SCHOMBURG

Biochem J (2004) 378 (1): 21–26.

https://doi.org/10.1042/bj20031795

Published: 15 February 2004

... sufficiently safe to prevent overdosages. Key words: glutathione peroxidase, growth, lactation, selenium, selenoprotein P, transport. INTRODUCTION Selenium (Se) is an essential trace element that is required for normal growth and development [1 3]. It is present in the rare amino acid selenocysteine (Sec...

View article titled, Efficient selenium transfer from mother to offspring in selenoprotein-P-deficient mice enables dose-dependent rescue of phenotypes associated with selenium deficiency

Articles

Use of the transport specificity ratio and cysteine-scanning mutagenesis to detect multiple substrate specificity determinants in the consensus amphipathic region of the Escherichia coli GABA (γ-aminobutyric acid) transporter encoded by gabP

Steven C. KING, Lisa BROWN-ISTVAN

Biochem J (2003) 376 (3): 633–644.

https://doi.org/10.1042/bj20030594

Published: 15 December 2003

...Steven C. KING; Lisa BROWN-ISTVAN The Escherichia coli GABA (γ-aminobutyric acid) permease, GabP, and other members of the APC (amine/polyamine/choline) transporter superfamily share a CAR (consensus amphipathic region) that probably contributes to solute translocation. If true, then the CAR should...

View article titled, Use of the transport specificity ratio and cysteine-scanning mutagenesis to detect multiple substrate specificity determinants in the consensus amphipathic region of the Escherichia coli GABA (γ-aminobutyric acid) transporter encoded by gabP

Articles

Induction of substrate specificity shifts by placement of alanine insertions within the consensus amphipathic region of the Escherichia coli GABA (γ-aminobutyric acid) transporter encoded by gabP

Steven C. KING, Liaoyuan A. HU, Amy PUGH

Biochem J (2003) 376 (3): 645–653.

https://doi.org/10.1042/bj20030595

Published: 15 December 2003

...Steven C. KING; Liaoyuan A. HU; Amy PUGH The Escherichia coli GABA (γ-aminobutyric acid) permease GabP is a prototypical APC (amine/polyamine/choline) super-family transporter that has a CAR (consensus amphipathic region) containing multiple specificity determinants, ostensibly organized on two...

View article titled, Induction of substrate specificity shifts by placement of alanine insertions within the consensus amphipathic region of the Escherichia coli GABA (γ-aminobutyric acid) transporter encoded by gabP

Articles

Polarized expression of members of the solute carrier SLC19A gene family of water-soluble multivitamin transporters: implications for physiological function

Michael J. BOULWARE, Veedamali S. SUBRAMANIAN, Hamid M. SAID, Jonathan S. MARCHANT

Biochem J (2003) 376 (1): 43–48.

https://doi.org/10.1042/bj20031220

Published: 15 November 2003

...Michael J. BOULWARE; Veedamali S. SUBRAMANIAN; Hamid M. SAID; Jonathan S. MARCHANT Humans lack biochemical pathways for the synthesis of the micro-nutrients thiamine and folate. Cellular requirements are met through membrane transport activity, which is mediated by proteins of the SLC19A gene...

View article titled, Polarized expression of members of the solute carrier SLC19A gene family of water-soluble multivitamin transporters: implications for physiological function

Articles

Role of organic anion-transporting polypeptides, OATP-A, OATP-C and OATP-8, in the human placenta-maternal liver tandem excretory pathway for foetal bilirubin

Oscar BRIZ, Maria A. SERRANO, Rocio I. R. MACIAS, Javier GONZALEZ-GALLEGO, Jose J. G. MARIN

Biochem J (2003) 371 (3): 897–905.

https://doi.org/10.1042/bj20030034

Published: 01 May 2003

...Oscar BRIZ; Maria A. SERRANO; Rocio I. R. MACIAS; Javier GONZALEZ-GALLEGO; Jose J. G. MARIN Recent functional studies have suggested that, in addition to simple diffusion, carrier-mediated transport may play an important role in foetal unconjugated bilirubin (UCB) uptake by the placenta. We have...

View article titled, Role of organic anion-transporting polypeptides, OATP-A, OATP-C and OATP-8, in the human placenta-maternal liver tandem excretory pathway for foetal bilirubin

Articles

Transport of bile acids in multidrug-resistance-protein 3-overexpressing cells co-transfected with the ileal Na+-dependent bile-acid transporter

Noam ZELCER, Tohru SAEKI, Ilse BOT, Annemieke KUIL, Piet BORST

Biochem J (2003) 369 (1): 23–30.

https://doi.org/10.1042/bj20021081

Published: 01 January 2003

...Noam ZELCER; Tohru SAEKI; Ilse BOT; Annemieke KUIL; Piet BORST Many of the transporters involved in the transport of bile acids in the enterohepatic circulation have been characterized. The basolateral bile-acid transporter of ileocytes and cholangiocytes remains an exception. It has been suggested...

View article titled, Transport of bile acids in multidrug-resistance-protein 3-overexpressing cells co-transfected with the ileal Na+-dependent bile-acid transporter

Articles

Comparative characterization of hexose transporters of Plasmodium knowlesi, Plasmodium yoelii and Toxoplasma gondii highlights functional differences within the apicomplexan family

Thierry JOËT, Lennart HOLTERMAN, Timothy T. STEDMAN, Clemens H.M. KOCKEN, Annemarie van der WEL, Alan W. THOMAS, Sanjeev KRISHNA

Biochem J (2002) 368 (3): 923–929.

https://doi.org/10.1042/bj20021189

Published: 15 December 2002

...Thierry JOËT; Lennart HOLTERMAN; Timothy T. STEDMAN; Clemens H.M. KOCKEN; Annemarie van der WEL; Alan W. THOMAS; Sanjeev KRISHNA Chemotherapy of apicomplexan parasites is limited by emerging drug resistance or lack of novel targets. PfHT1, the Plasmodium falciparum hexose transporter 1...

View article titled, Comparative characterization of hexose transporters of Plasmodium knowlesi, Plasmodium yoelii and Toxoplasma gondii highlights functional differences within the apicomplexan family

Articles

Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metabolite

Alison V. FRASER, Patrick M. WOSTER, Heather M. WALLACE

Biochem J (2002) 367 (1): 307–312.

https://doi.org/10.1042/bj20020156

Published: 01 October 2002

... transporter system for uptake and its accumulation in cells was prevented by polyamine transport inhibitors. IPENSpm can be classified as a polyamine anti-metabolite and it may be a promising new lead compound in terms of treatment of some human cancers. Biochem. J. (2002) 367, 307 312 (Printed in Great...

View article titled, Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metabolite

Articles

Perturbation of free oligosaccharide trafficking in endoplasmic reticulum glucosidase I-deficient and castanospermine-treated cells

Christelle DURRANT, Stuart E.H. MOORE

Biochem J (2002) 365 (1): 239–247.

https://doi.org/10.1042/bj20011786

Published: 01 July 2002

... where they, along with their cytosolically generated counterparts possessing a single N -acetylglucosamine residue at their reducing termini (FOSGN1), are trimmed in order to be imported into lysosomes for final degradation. Both the ER and lysosomal FOS transport processes are unable to translocate...

View article titled, Perturbation of free oligosaccharide trafficking in endoplasmic reticulum glucosidase I-deficient and castanospermine-treated cells

Articles

Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae

Sandra PAIVA, Arthur L. KRUCKEBERG, Margarida CASAL

Biochem J (2002) 363 (3): 737–744.

https://doi.org/10.1042/bj3630737

Published: 24 April 2002

... transporter with a cellular abundance of 1670 molecules/cell, and a catalytic-centre activity of 123s −1 . It is expressed and tagged to the plasma membrane under induction conditions. The factors involved in proper localization and turnover of Jen1p were revealed by expression of the Jen1p—GFP fusion protein...

View article titled, Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae

Articles

Antimonite regulation of the ATPase activity of ArsA, the catalytic subunit of the arsenical pump

Adrian R. WALMSLEY, Tongqing ZHOU, M. Ines BORGES-WALMSLEY, Barry P. ROSEN

Biochem J (2001) 360 (3): 589–597.

https://doi.org/10.1042/bj3600589

Published: 10 December 2001

... members of the ATP- binding cassette (ABC) superfamily of membrane transporters are drug-translocating ATPases that pump drugs out of the cytosol, thus conferring drug resistance in both prokaryotes and eukaryotes. This family includes the human multidrug-resistance ( MDR ) P-glycoprotein, which confers...

View article titled, Antimonite regulation of the ATPase activity of ArsA, the catalytic subunit of the arsenical pump

Articles

The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush-border membrane

George L. KELLETT, Philip A. HELLIWELL

Biochem J (2000) 350 (1): 155–162.

https://doi.org/10.1042/bj3500155

Published: 09 August 2000

...-operative ( n = 1.6, [G 1/2 ] = 56mM; where [G 1/2 ] is the glucose concentration at half V max ) because of the glucose-induced activation and recruitment of GLUT2 to the brush-border membrane. Diffusive transport by paracellular flow was negligible. The phloretin-insensitive, SGLT1-mediated, component...

View article titled, The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush-border membrane

Articles

Regulation of GLUT5, GLUT2 and intestinal brush-border fructose absorption by the extracellular signal-regulated kinase, p38 mitogen-activated kinase and phosphatidylinositol 3-kinase intracellular signalling pathways: implications for adaptation to diabetes

Philip A. HELLIWELL, Michael RICHARDSON, Julie AFFLECK, George L. KELLETT

Biochem J (2000) 350 (1): 163–169.

https://doi.org/10.1042/bj3500163

Published: 09 August 2000

...Philip A. HELLIWELL; Michael RICHARDSON; Julie AFFLECK; George L. KELLETT We have investigated the role of the extracellular signal-regulated kinase (ERK), p38 and phosphatidylinositol 3-kinase (PI 3-kinase) pathways in the regulation of intestinal fructose transport. Different combinations...

View article titled, Regulation of GLUT5, GLUT2 and intestinal brush-border fructose absorption by the extracellular signal-regulated kinase, p38 mitogen-activated kinase and phosphatidylinositol 3-kinase intracellular signalling pathways: implications for adaptation to diabetes

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only