...Julia Tschirka; Markus Bach; Ilmars Kisis; Julia Lemmen; Mark Jean Gnoth; Dirk Gründemann The transport efficiency (TE) describes the performance of a transport protein for a specific substrate. To compare the TE of different transporters, the number of active transporters in the plasma membrane...

... are not involved in preselection of metal substrate but play a role in promoting zinc transport. The possible function of the histidine-rich loop as a metal chaperone facilitating zinc binding to the transport site and/or a zinc sensor allosterically regulating the transport machinery was discussed. This work...

...Mònica Rius; Laura Sala; Josep Chillarón The transport system b 0,+ mediates reabsorption of dibasic amino acids and cystine in the kidney. It is made up of two disulfide-linked membrane subunits: the carrier, b 0,+ AT and the helper, rBAT (related to b 0,+ amino acid transporter). rBAT mutations...

...Mike R. Wilson; Sita Kugel; Jenny Huang; Lucas J. Wilson; Patrick A. Wloszczynski; Jun Ye; Larry H. Matherly; Zhanjun Hou The human proton-coupled folate transporter (hPCFT) is expressed in solid tumours and is active at pHs characterizing the tumour microenvironment. Recent attention focused...

...Emma L. R. Compton; Kimberly Page; Heather E. Findlay; Michael Haertlein; Martine Moulin; Ulrich Zachariae; David G. Norman; Frank Gabel; Arnaud Javelle The Slc26 proteins are a ubiquitous superfamily of anion transporters conserved from bacteria to humans, among which four have been identified...

...Christoph Mathieu; Amaia González Salgado; Corina Wirdnam; Stefan Meier; Marianne Suter Grotemeyer; Ehud Inbar; Pascal Mäser; Dan Zilberstein; Erwin Sigel; Peter Bütikofer; Doris Rentsch Amino acid transporters are crucial for parasite survival since the cellular metabolism of parasitic protozoa...

... transport proteins, integral membrane proteins that mediate the passage of solutes across the various membranes that separate the biochemical machinery of the parasite from the extracellular environment. Proteins of this type play a key role in antimalarial drug resistance, as well as being candidate drug...

... the substrate-binding groove, perhaps explaining the narrower substrate specificity of the enzyme compared with other zinc carboxypeptidases. In γ-proteobacteria mpaA is often located adjacent to mppA which encodes a periplasmic transporter protein previously shown to bind murein tripeptide. We demonstrate...

...Stefan Bröer Metabolism in tumour cells is adapted to the demands of a growing cell. The Warburg effect and increased use of glutamine are two well-known adaptations of tumour metabolism. Both require transporters to allow uptake of substrates and efflux of products. Differentiated cells are less...

...Tyler Mandt; Yumei Song; Andrew M. Scharenberg; Jaya Sahni SLC41A1 (solute carrier family 41, member A1) is a recently described vertebrate member of the MgtE family of Mg 2+ transporters. Although MgtE transporters are found in both prokaryotic and eukaryotic organisms, and are highly conserved...

...Zhanjun Hou; Jianmei Wu; Jun Ye; Christina Cherian; Larry H. Matherly RFC (reduced folate carrier) is the major transporter for reduced folates and antifolates [e.g. MTX (methotrexate)]. RFC is characterized by two halves, each with six TMD (transmembrane domain) α helices connected...

...Meaghan STOLK; Elizabeth COOPER; Greg VILK; David W. LITCHFIELD; James R. HAMMOND Two subtypes of equilibrative transporters, es (equilibrative inhibitor-sensitive) and ei (equilibrative inhibitor-insensitive), are responsible for the majority of nucleoside flux across mammalian cell membranes...

... of cancer cells to the drug is not known. In the present study, we created fluorescently labelled BLM-A5, which retained nearly full genotoxic potential, and used this molecule to conduct the first study to understand the transport pathway of the drug in Saccharomyces cerevisiae . Uptake studies revealed...

...Claire I. BUNGARD; John D. McGIVAN Glutamine transport into the human hepatoma cell line HepG2 is catalysed primarily by an ASCT2-type transporter identical in sequence with that cloned previously from JAR cells. An antibody raised against the C-terminus of the ASCT2 protein was shown to recognize...

... is required to facilitate AEA transport across the plasma membrane in a selective and bi-directional way. Abbreviations used: AEA, N -arachidonoylethanolamine (anandamide); 2-AG, 2-arachidonoylglycerol; FAAH, fatty acid amide hydrolase; HEK-293 cells, human embryonic kidney 293 cells; NADA, N...

...Wei CAO; Larry H. MATHERLY The hRFC (human reduced folate carrier) is the major membrane transporter for both reduced folates and antifolates in human tissues and tumours. The primary amino acid sequence of hRFC predicts a membrane topology involving 12 TMDs (transmembrane domains) with cytosolic...

...Rosa J. TORRES; Isabel DeANTONIO; Carmen PRIOR; Juan G. PUIG We postulated that adenosine function could be related to some of the neurological features of Lesch–Nyhan syndrome and therefore characterized adenosine transport in PBLs (peripheral blood lymphocytes) obtained from Lesch–Nyhan patients...

...Hideo SATSU; Eriko TERASAWA; Yu HOSOKAWA; Makoto SHIMIZU We investigated the characterization and the regulation of TAUT (taurine transporter) and CDO (cysteine dioxygenase), one of the key enzymes of taurine biosynthesis, in human hepatoblastoma HepG2 cells. The activity of TAUT in the HepG2 cells...

...Wei CAO; Larry H. MATHERLY The human reduced folate carrier (hRFC) mediates the transport of reduced folates and classical anti-folates into mammalian cells. Whereas the functionally important domains in hRFC are poorly characterized, previous studies with anti-folate-resistant cells suggest...

[email protected] ). 21 5 2002 7 7 2002 24 7 2002 24 7 2002 The Biochemical Society, London ©2002 2002 drug resistance mutation transporter Abbreviations used: 10-CHOTHF, 10-formyltetrahydrofolate; CH 2 THF, N 5 , N 10 -methylenetetrahydrofolate; hRFC, human RFC; MRP1...

...]. Substrate would be provided to this enzyme by a translocase that is specific for glucose 6-phosphate, thereby accounting for the specificity of the phosphatase for glucose 6-phosphate in intact microsomes. Distinct transporters would allow inorganic phosphate and glucose to leave the vesicles. At variance...

... of the endogenous cannabinoid system (ECS). Here we synthesized a series of N -acyl-dopamines (NADAs) and studied their effects on the anandamide membrane transporter, the anandamide amidohydrolase (fatty acid amide hydrolase, FAAH) and the two cannabinoid receptor subtypes, CB 1 and CB 2 . NADAs competitively...

...Benoît PINSON; Jean CHEVALLIER; Danièle URBAN-GRIMAL The transport of uracil into the yeast Saccharomyces cerevisiae is mediated by uracil permease, a specific co-transporter encoded by the FUR4 gene. Uracil permease is a multispan membrane protein that is delivered to the plasma membrane via...