... described. Null mutant parasites for LABCG2 present an increase in the intracellular levels of glutathione (GSH) and trypanothione [T(SH) 2 ]. On the other hand, parasites overexpressing LABCG2 transporter export non-protein thiols to the extracellular medium. To explore if LABCG2 may mediate an active...

... drug discovery glutathione peroxidase Leishmania Trypanosoma trypanothione Human African trypanosomiasis (sleeping sickness) is responsible for an estimated 80000 new cases and 30000 deaths each year [ 1 ]. Current therapeutics against the protozoan parasite ( Trypanosoma brucei sspp...

..., which are essential for the parasites, lack glutathione peroxidase activity but catalyse the trypanothione/Tpx (tryparedoxin)-dependent reduction of hydroperoxides. Cys 47 , Gln 82 and Trp 137 correspond to the selenocysteine, glutamine and tryptophan catalytic triad of the mammalian selenoenzymes. Site...

... impaired when TXN was lowered to 1 μM for more than 48 h. Although the levels of glutathione, glutathionylspermidine and trypanothione were increased 2–3.5-fold, the sensitivity against exogenously generated H 2 O 2 was significantly enhanced. The results prove the essential role of the cTXN and its...

.... donovani GLOII showed a marked substrate specificity for trypanothione hemithioacetal over glutathione hemithioacetal. Antiserum against recombinant LdGLOII protein could detect a band of anticipated size ∼32 kDa in promastigote extracts. By overexpressing the GLOII gene in Leishmania donovani using...

...Mark R. Ariyanayagam; Sandra L. Oza; Maria Lucia S. Guther; Alan H. Fairlamb Trypanothione plays a pivotal role in defence against chemical and oxidant stress, thiol redox homoeostasis, ribonucleotide metabolism and drug resistance in parasitic kinetoplastids. In Trypanosoma brucei , trypanothione...

... Nucleotide Sequence Database under accession number AJ313313. 7 1 2002 22 2 2002 4 4 2002 The Biochemical Society, London ©2002 2002 hydroperoxide metabolism reactive oxygen species trypanothione Abbreviations used: ROS, reactive oxygen species; GPX, glutathione...

... . The enzyme was shown to have peroxidase activity in the presence of glutathione/glutathione reductase but not in the presence of trypanothione/trypanothione reductase. It could metabolize a wide range of hydroperoxides (linoleic acid hydroperoxide and phosphatidylcholine hydroperoxide> cumene...