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Keywords: ubiquitination
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Biochem J (2023) 480 (20): 1659–1674.
Published: 19 October 2023
...-proteomics revealed over 1000 potential mechanosensing TAFs and UBE2A/B (Ubiquitin-conjugating enzyme E2 A) was experimentally identified as a force- and CI-dependent nucleocytoplasmic shuttling TAF. We found that translocation of YAP/TAZ and UBE2A/B are distinctively regulated by inhibition of myosin...
Includes: Supplementary data
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Biochem J (2022) 479 (22): 2379–2394.
Published: 30 November 2022
... been shown to be regulated primarily through phosphorylation and ubiquitination during various stages of the cell cycle. Although phosphorylation and ubiquitin-dependent proteasomal degradation of p21 have been well established, other post-translational modifications that contribute to regulation...
Includes: Supplementary data
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Biochem J (2019) 476 (13): 1911–1926.
Published: 02 July 2019
... of the conserved H-bond between Y210 and E237 of ERK1 through point mutation at or naturally occurring nitration on Y210 initiates a quality control program dependent on chaperon systems and CHIP (C-terminal of Hsp70-interacting protein)-mediated ubiquitination and degradation. The H-bond is also important...
Includes: Supplementary data
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Biochem J (2017) 474 (22): 3747–3761.
Published: 01 November 2017
... interaction ubiquitination The human KCTD family contains 25 soluble proteins that share a conserved potassium ( K + ) C hannel T etramerization D omain (a subtype of ‘BTB domain’) at their N-termini and have variable C-termini [ 1 ]. The core BTB fold (named after homologous regions...
Includes: Supplementary data
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Biochem J (2016) 473 (10): 1297–1314.
Published: 11 May 2016
...Mathew Stanley; Satpal Virdee The modification of proteins with ubiquitin (Ub) is an important regulator of eukaryotic biology and deleterious perturbation of this process is widely linked to the onset of various diseases. The regulatory capacity of the Ub signal is high and, in part, arises from...
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Biochem J (2016) 473 (5): 571–580.
Published: 24 February 2016
... there being no change in either the levels or the translation of their mRNAs. Instead, these subunits are targeted for degradation by the ubiquitin–proteasome system. The data allow us to propose a model for the formation of stoichiometric eIF2B complexes which can ensure their stoichiometric incorporation...
Includes: Supplementary data
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Biochem J (2015) 471 (2): 243–253.
Published: 02 October 2015
... by cholesterol accumulation in late endosomes (LE) and lysosomes (Ly). Nascent or mutated NPC1 is degraded through the ubiquitin–proteasome pathway, but how NPC1 degradation is regulated remains currently unknown. In the present study, we demonstrated a link between NPC1 degradation and the Akt (protein kinase B...
Includes: Supplementary data
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Biochem J (2015) 467 (3): 365–386.
Published: 17 April 2015
...Emil Bulatov; Alessio Ciulli In the last decade, the ubiquitin–proteasome system has emerged as a valid target for the development of novel therapeutics. E3 ubiquitin ligases are particularly attractive targets because they confer substrate specificity on the ubiquitin system. CRLs [Cullin–RING...
Includes: Supplementary data
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Biochem J (2014) 463 (1): e1–e2.
Published: 08 September 2014
...) phosphorylation ubiquitination 1 To whom correspondence should be addressed (email rudi.beyaert@irc.vib-ugent.be ). 1 8 2014 5 8 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 The NF-κB (nuclear factor κB) transcription factor family controls...
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Biochem J (2014) 461 (3): 435–442.
Published: 10 July 2014
... at the post-translational level by accelerated cholesterol-dependent ubiquitination and proteasomal degradation, which is associated with the accumulation of squalene. Using model cell systems, we report that SM is stabilized by unsaturated fatty acids. Treatment with unsaturated fatty acids such as oleate...
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Biochem J (2014) 461 (1): 137–146.
Published: 13 June 2014
... of oxidative stress in vitro and in vivo , suggesting that Drp1 might be a useful target to combat necrosis in human disease. dynamin-related protein 1 (Drp1) mitochondrion necrosis oxidative stress p53 ubiquitination 1 To whom correspondence should be addressed (email xxq38...
Includes: Supplementary data
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Biochem J (2014) 459 (2): 301–312.
Published: 28 March 2014
... (SLC10A1), OSTα (SLC51A) and ABCG1 (ATP-binding cassette G1), remained unaffected. ASBT inhibition by RSV was reversed by proteasome inhibitors (MG-132 and lactacystin) and the ubiquitin inhibitor LDN57444, suggesting involvement of the ubiquitin–proteasome pathway. Immunoprecipitation revealed high levels...
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Biochem J (2014) 459 (1): 193–203.
Published: 14 March 2014
... enzymes. To date, little is known about the upstream regulation of hPXR. Using MS analysis and a kinome-wide siRNA screen, we report that the E3 ligase UBR5 (ubiquitin protein ligase E3 component n-recognin 5) and DYRK2 (dual-specificity tyrosine-phosphorylation-regulated kinase 2) regulate hPXR stability...
Includes: Supplementary data
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Biochem J (2014) 458 (3): 421–437.
Published: 28 February 2014
...Donald E. Spratt; Helen Walden; Gary S. Shaw The RBR (RING-BetweenRING-RING) or TRIAD [two RING fingers and a DRIL (double RING finger linked)] E3 ubiquitin ligases comprise a group of 12 complex multidomain enzymes. This unique family of E3 ligases includes parkin, whose dysfunction is linked...
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Biochem J (2014) 458 (3): 537–545.
Published: 28 February 2014
... ubiquitination of p21 in a reconstituted in vitro system where UbcH5a is the preferred E2. Thus the ability of TRIM3 to suppress growth is associated with its ability to ubiquitinate proteins. © The Authors Journal compilation © 2014 Biochemical Society 2014 The present study demonstrates that growth...
Includes: Supplementary data
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Biochem J (2013) 456 (1): 55–66.
Published: 24 October 2013
...), as a VHL-interacting protein. GST pull-down and co-immunoprecipitation assays show that UXT interacts with VHL. In addition, UXT recruits VHL to the nucleus. VHL associates with the DBD (DNA-binding domain) and hinge domains of the AR and induces AR ubiquitination. Moreover, VHL interaction with the AR...
Includes: Supplementary data
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Biochem J (2013) 453 (3): 345–356.
Published: 12 July 2013
... studies indicated that PSD95 enhanced Mas protein expression by increasing the stabilization of the receptor. Mas degradation was robustly inhibited by the proteasome inhibitor MG132 in time- and dose-dependent manners, and the expression of PSD95 impaired Mas ubiquitination, indicating that the PSD95–Mas...
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Biochem J (2013) 452 (1): 139–145.
Published: 25 April 2013
...) Nck ubiquitination We further measured whether FGF-2 has an impact on ER stress-mediated apoptosis signalling such as CHOP (also known as DDIT3 and GADD153) expression and PARP cleavage. Both the induction of CHOP expression and PARP cleavage observed on either TM or TG treatment were blocked...
Includes: Supplementary data
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Biochem J (2013) 449 (3): 707–717.
Published: 09 January 2013
...Vivien Landré; Emmanuelle Pion; Vikram Narayan; Dimitris P. Xirodimas; Kathryn L. Ball Understanding the determinants for site-specific ubiquitination by E3 ligase components of the ubiquitin machinery is proving to be a challenge. In the present study we investigate the role of an E3 ligase...
Includes: Supplementary data
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Biochem J (2013) 449 (2): 365–371.
Published: 14 December 2012
... describe the generation of recombinant human APC/C (anaphase-promoting complex/cyclosome), an E3 ubiquitin ligase that regulates cell-cycle progression. Human APC/C is composed of 14 distinct proteins that assemble into a complex of at least 19 subunits with a combined molecular mass of ~1.2 MDa. We show...
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Biochem J (2012) 443 (3): 681–689.
Published: 16 April 2012
... (ubiquitin/proteasome system). Moreover, the ubiquitination level of mutant Htt was found to be enhanced when FE65 is knocked down. Immunofluroescence staining revealed that FE65 associates with mutant Htt aggregates. Additionally, we demonstrated that overexpression of FE65 increases mutant Htt-induced cell...
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Biochem J (2012) 441 (3): 979–987.
Published: 16 January 2012
.... Polyubiquitination of TRAF2 and TRAF6 is critical to their activities and functions in TNFα- and IL (interleukin)-1β-induced NF-κB activation. However, the regulation of TRAF2 and TRAF6 by deubiquitination remains incompletely understood. In the present study, we identified USP (ubiquitin-specific protease) 4...
Includes: Supplementary data
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Biochem J (2012) 441 (1): 399–406.
Published: 14 December 2011
.... Interestingly, not only reduced expression but also overexpression of Beclin 1 is correlated with cancer development and metastasis. Thus it seems necessary for the cell to balance the protein levels of Beclin 1. In the present study we describe a regulatory link between Beclin 1 and the ubiquitin ligase Nedd4...
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Biochem J (2011) 437 (2): 255–267.
Published: 28 June 2011
... implicated in the internalization of the protein. In recent years, ubiquitination of Cx43 has also been proposed to regulate gap junction intercellular communication; however, the underlying mechanism and molecular players involved remain elusive. In the present study, we demonstrate that ubiquitinated Cx43...
Includes: Multimedia, Supplementary data
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Biochem J (2011) 434 (2): 275–285.
Published: 11 February 2011
... checkpoint’ function through both p53-dependent and p53-independent mechanisms. In the present study, we demonstrate a novel p53-independent interaction between p14 ARF and the adenovirus oncoprotein E1A. p14 ARF inhibits E1A transcriptional function and promotes ubiquitination-dependent degradation of E1A...
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Biochem J (2011) 433 (1): 31–42.
Published: 15 December 2010
...Dawn M. Wenzel; Kate E. Stoll; Rachel E. Klevit Ubiquitination is a post-translational modification pathway involved in myriad cellular regulation and disease pathways. The Ub (ubiquitin) transfer cascade requires three enzyme activities: a Ub-activating (E1) enzyme, a Ub-conjugating (E2) enzyme...
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Biochem J (2010) 427 (1): 91–104.
Published: 15 March 2010
... and Sug1 contain caspase 8. Expression of Ac-Ipaf or co-expression of Sug1 with Ipaf results in the formation of cytoplasmic aggregates and caspase 8 activation. Sug1 co-expression enabled modification of Ipaf by ubiquitination. Tagging ubiquitin molecules to Ipaf led to aggregate formation, enhanced...
Includes: Supplementary data
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Biochem J (2009) 422 (1): 1–10.
Published: 29 July 2009
.... Modifications of TLRs and their receptor proximal signalling proteins have also been uncovered. Phosphorylation of adaptor proteins and ubiquitination (both Lys 48 - and Lys 63 -linked) of TLRs, IRAKs (interleukin-1 receptor-associated kinase), Pellinos and TRAF6 (tumour-necrosis-factor-receptor-associated...
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Biochem J (2008) 416 (1): 117–127.
Published: 28 October 2008
...-associated protein) complex to NF-κB target genes. Importantly, the molecular mechanisms which determine the functions of RelB are still largely unknown. In the present study, we aimed to analyse whether ubiquitination of RelB might be involved in the regulation of RelB. Indeed, RelB is constitutively...
Includes: Supplementary data
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Biochem J (2008) 414 (2): 221–229.
Published: 12 August 2008
... and regulation of PTEN ubiquitination catalysed by NEDD4-1 (neural-precursor-cell-expressed, developmentally down-regulated 4-1), a ubiquitin ligase for PTEN we identified recently. Using the reconstituted assay and cellular analysis, we demonstrated that NEDD4-1-mediated PTEN ubiquitination depends on its...
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Biochem J (2008) 411 (2): e9–e10.
Published: 27 March 2008
... AMPK-related kinases are less well defined. AMP kinase (AMPK) deubiquitination LKB1 kinase signal transduction specificity ubiquitination Since LKB1 activates a number of kinases in the AMPK-related kinase family, how is signal specificity and context generated? Interestingly, LKB1...
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Biochem J (2008) 411 (2): 249–260.
Published: 27 March 2008
...-related kinases, NUAK1 (AMPK-related kinase 5) and MARK4 (microtubule-affinity-regulating kinase 4), are polyubiquitinated in vivo and interact with the deubiquitinating enzyme USP9X (ubiquitin specific protease-9). Knockdown of USP9X increased polyubiquitination of NUAK1 and MARK4, whereas overexpression...
Includes: Supplementary data
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Biochem J (2008) 410 (3): 585–594.
Published: 27 February 2008
...-R trafficking and degradation. EGF (17 nM) and BTC (8.5 nM) induced significant EGF-R degradation, with or without ectopic expression of the ubiquitin ligase Cbl. Human recombinant AR (17 nM) failed to affect receptor degradation in either case. Notably, levels of ligand-induced EGF-R ubiquitination...
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Biochem J (2008) 410 (3): 439–453.
Published: 27 February 2008
...-chaperone protein activation and degradation ubiquitination Hsp90 (90 kDa heat-shock protein) is one of a group of molecular chaperones responsible for managing protein folding and quality control in the crowded environment of the cell [ 1 ]. Although Hsp90 is involved in the triage of misfolded...
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Biochem J (2008) 409 (1): 275–287.
Published: 11 December 2007
... or Box 1 motifs. As a further consequence of activation by 3M-003, TLR8 is modified to yield both higher and lower molecular mass species. These species include a monoubiquitinated form as deduced from ubiquitin peptide sequencing by HPLC/MS/MS (tandem MS). 1 To whom correspondence should...
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Biochem J (2006) 399 (3): 361–372.
Published: 13 October 2006
...James H. Hurley; Sangho Lee; Gali Prag The covalent modification of proteins by ubiquitination is a major regulatory mechanism of protein degradation and quality control, endocytosis, vesicular trafficking, cell-cycle control, stress response, DNA repair, growth-factor signalling, transcription...
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Biochem J (2006) 397 (1): 31–38.
Published: 14 June 2006
... of the E3 ubiquitin ligase, β-TrCP2 (β-transducin repeats-containing protein 2), ubiquitination of IFNAR1 and proteolysis. The non-catalytic role of Tyk2 in sustaining the steady-state IFNAR1 level at the plasma membrane is well documented; however, little is known about the function of Tyk2 in the steps...
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Biochem J (2006) 396 (3): 411–417.
Published: 29 May 2006
... domains including an N-terminal SWIM (SWI2/SNF2 and MuDR) domain of unknown function and two RING (really interesting new gene) fingers separated by a ZZ zinc finger domain. Biochemical analyses revealed that MEX is self-ubiquitinated and targeted for degradation through the proteasome pathway. MEX can...