... or development, and by which enzyme or in which subcellular compartment the InsP signal is generated. Characterising ubiquitin machinery components and the specificity of their interactions with signalling molecules and their isomers will be crucial for understanding how different responses might be elicited...

...Nicholas Purser; Ishita Tripathi-Giesgen; Jerry Li; Daniel C. Scott; Daniel Horn-Ghetko; Kheewoong Baek; Brenda A. Schulman; Arno F. Alpi; Gary Kleiger Protein ubiquitylation typically involves isopeptide bond formation between the C-terminus of ubiquitin to the side-chain amino group on Lys...

...Elisha H. McCrory; Vyacheslav Akimov; Philip Cohen; Blagoy Blagoev The E3 ligase HOIL-1 forms ester bonds in vitro between ubiquitin and serine/threonine residues in proteins. Here, we exploit UbiSite technology to identify serine and threonine residues undergoing HOIL-1 catalysed ubiquitylation...

... is as a RING-type E3 ubiquitin (Ub) ligase, leading to the deposition of Ub signals onto a variety of substrate proteins. Distinct types of Ub signals deposited by BRCA1/BARD1 (i.e. degradative vs. non-degradative; mono-Ub vs. poly-Ub chains) on substrate proteins mediate aspects of its function in DNA double...

...Thanh Thi Le; Johanna Ainsworth; Cristian Polo Rivera; Thomas Macartney; Karim P.M. Labib Cullin ubiquitin ligases drive replisome disassembly during DNA replication termination. In worm, frog and mouse cells, CUL2 LRR1 is required to ubiquitylate the MCM7 subunit of the CMG helicase. Here, we show...

...Marina Ikeda; Tadashi Watanabe; Akihiro Ito; Masahiro Fujimuro Herpes simplex virus 1 (HSV-1) is a human DNA virus that causes cold sores, keratitis, meningitis, and encephalitis. Ubiquitination is a post-translational protein modification essential for regulation of cellular events...

... is sequential ubiquitination of proliferating cell nuclear antigen (PCNA), a DNA clamp required for replication and DNA repair. DDT consists of two parallel pathways: error-prone DDT is mediated by PCNA monoubiquitination, which recruits translesion synthesis DNA polymerases to bypass lesions with decreased...

...Hitendra Negi; Pothula Purushotham Reddy; Vineeth Vengayil; Chhaya Patole; Sunil Laxman; Ranabir Das The Baculoviridae family of viruses encode a viral Ubiquitin (vUb) gene. Though the vUb is homologous to the host eukaryotic Ubiquitin (Ub), its preservation in the viral genome indicates unique...

.... The ubiquitination of protein needs to be treated with or without 10 μM MG132 for an additional 6 h to block proteasomal degradation of protein after transfection for 24 h. Then the co-immunoprecipitation and Western blotting assays were performed as previously reported [ 25 ]. © 2020 The Author(s). Published...

...Sayani Sarkar; Adaitya Prasad Behera; Prateeka Borar; Prerana Agarwal Banka; Ajit B. Datta Ubiquitin RING E3 ligases (E3s) catalyze ubiquitin (Ub) transfer to their substrates by engaging E2∼Ub intermediates with the help of their RING domains. Different E3s have been found to contain a conserved...

...Adaitya Prasad Behera; Pritam Naskar; Shubhangi Agarwal; Prerana Agarwal Banka; Asim Poddar; Ajit B. Datta RING ( R eally I nteresting N ew G ene) domains in ubiquitin RING E3 ligases exclusively engage ubiquitin (Ub)-loaded E2s to facilitate ubiquitination of their substrates. Despite...

... proteins could affect the circadian behavior. Although ubiquitination of core clock proteins was studied extensively, the reverse process, deubiquitination, has only begun to unfold and the role of this regulation on circadian function is not completely understood. Here, we use affinity purification...