... biofortification iron nutrition remobilization seed vacuole The seed is a very special stage in the life of a plant. It is the basis for the next generation and the survival of the species. To ensure the success of the new generation, the mother plant transfers a large proportion of nutrients it has...

...Heejeong Lee; Jae Sam Hwang; Dong Gun Lee Communications between various organelle–organelles play an essential role in cell survival. The cross-talk between mitochondria and vacuoles comes up with the vital roles of the intercompartmental process. In this study, we found a couple of cell death...

... cells with DHA. We previously reported that MG depolarized the actin cytoskeleton and changed vacuolar morphology. We herein demonstrated the depolarization of actin and morphological changes in vacuoles following a treatment with DHA. Furthermore, we found that both MG and DHA caused the morphological...

... regulation vacuolar proton-translocating ATPase (V-ATPase) vacuole The V-ATPase (vacuolar proton-translocating ATPase) is a nanometre-scale molecular machine that performs rotational catalysis to drive protons across membranes. The ATP-hydrolysis motor V 1 and the proton turbine V o...

... molecules. RhoGDIs can also extract membrane-bound Rho GTPases to form soluble cytosolic complexes. We have shown previously that purified yeast RhoGDI Rdi1p, can inhibit vacuole membrane fusion in vitro . In the present paper we functionally dissect Rdi1p to discover its mode of regulating membrane fusion...

... and isomerizing disulfide bonds, as well as functioning as chaperones to newly synthesized proteins. In the present study we demonstrate that an abundant 7S lectin of the castor oil seed protein storage vacuole, RCA ( Ricinus communis agglutinin 1), is folded in the ER as disulfide bonded A–B dimers in both...

... 18 6 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 cation diffusion facilitator iron manganese transition metal vacuole yeast zinc Transition metal ions (Cu +/2+ , Fe 2+ , Zn 2+ , Co 2+ and Mn 2+ ) are essential for all organisms as they serve...

... is conserved from yeast to filamentous fungi. © The Authors Journal compilation © 2007 Biochemical Society 2007 Fusarium graminearum iron iron transporter Saccharomyces cerevisiae vacuole 2 To whom correspondence should be addressed (email [email protected] ). 2 4 2007...

...) syndrome. However, the functions of eukaryotic ACDPs remain unknown. We show here that S. cerevisiae MAM3 encodes an integral membrane protein of the yeast vacuole whose expression levels directly correlate with the degree of manganese toxicity. Surprisingly, Mam3p contributes to manganese toxicity without...

... on these results, self-aggregation of sophoragrin was shown to be a unique homopolymerization due to the sugar-specific interaction. An immunostaining study indicated that sophoragrin localizes mainly in vacuoles of parenchymal cells coincidently with B-SJA-I. These results indicate that sophoragrin can sequester...

..., vacuole fusion and structure. Vacuoles in Acb1p-depleted cells are multi-lobed, contain significantly less of the SNAREs (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptors) Nyv1p, Vam3p and Vti1p, and are unable to fuse in vitro . Mass spectrometric analysis revealed...

... cytoplasmic vacuoles along with disruption of the f-actin network. Interestingly, the truncated variants also imparted identical morphological changes. Characterization of the cytoplasmic vacuoles revealed that most of these vacuoles were autophagic in nature, resembling those generated under stress...

... mannosidase vacuole Abbreviations used: Man, mannose; Glc, glucose; GlcNAc, N -acetylglucosamine; fOS, free oligosaccharide(s); LLO, lipid-linked oligosaccharide; ER, endoplasmic reticulum; endo H, endo-β- d - N -acetylglucosamine H; AP, 2-aminopyridine; OST, oligosaccharyltransferase; ERAD...

...Su J. YANG; Shih S. JIANG; Soong Y. KUO; Shu H. HUNG; Ming F. TAM; Rong L. PAN A vacuolar H + -pyrophosphatase (EC 3.6.1.1) that catalyses PP i hydrolysis and the electrogenic translocation of protons from the cytosol to the vacuole lumen, was purified from etiolated hypocotyls of mung bean...