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Keywords: validation
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Articles
Biochem J (2024) 481 (10): 643–651.
Published: 10 May 2024
... these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or ex vivo...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (2): 323–332.
Published: 28 March 2014
... pharmacologically validated as a drug target in the African trypanosome, Trypanosoma brucei . In the present study, we evaluate NMT as a potential drug target in Trypanosoma cruzi , the causative agent of Chagas’ disease, using chemical and genetic approaches. Replacement of both allelic copies of TcNMT ( T. cruzi...