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Keywords: vitamin B1
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In silico , in vitro, and in vivo characterization of thiamin-binding proteins from plant seeds

Maria Faustino, Simon Strobbe, Raul Sanchez-Muñoz, Da Cao, Ratnesh C. Mishra, Tiago Lourenço, M. Margarida Oliveira, Dominique Van Der Straeten
Journal: Biochemical Journal
Biochem J (2025) 482 (04): 181–195.
https://doi.org/10.1042/BCJ20240429
Published: 05 February 2025
... diphosphate TMP thiamin monophosphate molecular docking seed storage protein thermal-shift assay thiamin vitamin B1 References 1 Whitfield , K.C. , Bourassa , M.W. , Adamolekun , B. , Bergeron , G. , Bettendorff , L. , Brown , K.H. et al...
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Includes: Supplementary data
Articles

A strictly monofunctional bacterial hydroxymethylpyrimidine phosphate kinase precludes damaging errors in thiamin biosynthesis

Antje M. Thamm, Gengnan Li, Marlene Taja-Moreno, Svetlana Y. Gerdes, Valérie de Crécy-Lagard, Steven D. Bruner, Andrew D. Hanson
Journal: Biochemical Journal
Biochem J (2017) 474 (16): 2887–2895.
https://doi.org/10.1042/BCJ20170437
Published: 10 August 2017
... require dietary thiamin that they convert directly to ThDP [ 1 , 2 ]. The enzymes of thiamin synthesis are consequently potential antimicrobial drug targets [ 1 , 2 ]. 4-amino-5-hydroxymethyl-2-methylpyrimidine kinetic characterization salvage thiamin vitamin B1 Plasmids encoding ThiD2...
View article titled, A strictly monofunctional bacterial hydroxymethylpyrimidine phosphate kinase precludes damaging errors in thiamin biosynthesis
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Includes: Supplementary data
Articles

Bacterial and plant HAD enzymes catalyse a missing phosphatase step in thiamin diphosphate biosynthesis

Ghulam Hasnain, Sanja Roje, Na Sa, Rémi Zallot, Michael J. Ziemak, Valérie de Crécy-Lagard, Jesse F. Gregory, III, Andrew D. Hanson
Journal: Biochemical Journal
Biochem J (2016) 473 (2): 157–166.
https://doi.org/10.1042/BJ20150805
Published: 05 January 2016
... vitamin B1 Thiamin diphosphate (ThDP) is an essential cofactor for central metabolic enzymes that include α-keto acid dehydrogenases, transketolase, pyruvate decarboxylase and acetolactate synthase [ 1 ]. ThDP is synthesized de novo by prokaryotes, plants and fungi [ 2 ]. In all...
View article titled, Bacterial and plant HAD enzymes catalyse a missing phosphatase step in thiamin diphosphate biosynthesis
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Includes: Supplementary data
Articles

A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism

Aymeric Goyer, Ghulam Hasnain, Océane Frelin, Maria A. Ralat, Jesse F. Gregory, III, Andrew D. Hanson
Journal: Biochemical Journal
Biochem J (2013) 454 (3): 533–542.
https://doi.org/10.1042/BJ20130516
Published: 29 August 2013
View article titled, A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism
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Includes: Supplementary data