... of methyltransferase activity. Using a combination of truncation mutants, a yeast two-hybrid system, and direct in vitro binding assays, we show that the human SUV39H1 chromodomain (CD) and HP1 chromoshadow domain (CSD) directly interact. Chaetocin inhibits this binding interaction through its disulfide functionality...

... a yeast two-hybrid screening using CCN2/CTGF peptide as a bait and a cDNA library from a chondrocytic cell line, HCS-2/8. In the present paper, we report the identification of aggrecan, which is a major proteoglycan of the extracellular matrix in cartilage, as a CCN2/CTGF-binding protein. Among the four...

... of these authors (email [email protected] or [email protected] ). Cdc13 DNA replication telomere binding telomere structure yeast two-hybrid In eukaryotic cells, the end of the linear chromosomes are composed of special nucleoprotein complexes known as telomeres, which are essential for chromosome...

... the molecular mechanism of transcriptional repression by the INSM1 gene, cyclin D1 was identified as an interacting protein by using a 45-day-old human foetal brain cDNA library and a yeast two-hybrid screen. The physical association between INSM1 and cyclin D1 was confirmed by in vitro and in vivo pull-down...

.... In the present study, we have investigated the ability of the four mammalian GRIP domain proteins to interact. Using a combination of immunoprecipitation experiments of epitope-tagged GRIP domain proteins, cross-linking experiments and yeast two-hybrid interactions, we have established that the GRIP proteins can...

...) microtubule-associated protein 1A (MAP1A) yeast two-hybrid Many extracellular physiological signalling agents increase the intracellular levels of the ubiquitous second-messenger cAMP [ 1 ]. These include immunomodulators, neurotransmitters and hormones, which typically activate the seven...

...Guozheng WANG; Shu ZHANG; David G. FERNIG; David SPILLER; Marisa MARTIN-FERNANDEZ; Hongmei ZHANG; Yi DING; Zihe RAO; Philip S. RUDLAND; Roger BARRACLOUGH With the widespread use of yeast two-hybrid systems, many heterodimeric forms of S100 proteins have been found, although their biological...

... cell migration. To examine the nischarin–α5 interaction in detail, and to find the minimal region required for the interaction, several mutants of nischarin and α5 were created. The results obtained for the yeast two-hybrid system indicate that a 99-aminoacid region of nischarin (from residues 464...

...Dongcheng LU; Henglin YAN; Timothy OTHMAN; Christopher P. TURNER; Thomas WOOLF; Scott A. RIVKEES To identify binding partners of the A 1 AR (A 1 adenosine receptor), yeast two-hybrid screening of a rat embryonic cDNA library was performed. This procedure led to the identification of erythrocyte...

... to the central control of energy homoeostasis. In order to identify putative MC4-R regulatory proteins, we performed a yeast two-hybrid screen of a mouse brain cDNA library using the mouse MC4-R intracellular tail (residues 303–332) as bait. We report here on one positive clone that shares 63% amino acid...

...Jeffrey A. SKINNER; Alan R. SALTIEL To identify novel protein phosphatase 1 (PP1)-interacting proteins, a yeast two-hybrid 3T3-L1 adipocyte cDNA library was screened with the catalytic subunit of PP1 as bait. In the present work, the isolation, identification and initial biochemical...