... partners of their cytoplasmic tail. We isolated Ubc9 (ubiquitin-conjugating enzyme 9) protein by screening a human kidney library using the yeast two-hybrid system. Lu/Ubc9 interaction was validated by GST (glutathione S-transferase) pull-down and co-immunoprecipitation experiments. Endogenous Ubc9 formed...

... ). 1 2 2006 20 6 2006 23 6 2006 23 6 2006 The Biochemical Society, London 2006 c-Fes coiled-coil KAP-1 non-receptor protein tyrosine kinase protein–protein interaction yeast two-hybrid system The human c-Fes locus is the cellular homologue of the transforming...

... events pivotal in inflammation. Whether CD39 interacts with other membrane or cytoplasmic proteins has not been established to date. Using the yeast two-hybrid system, we note that the N-terminus of CD39 binds to RanBPM (Ran binding protein M; also known as RanBP9), a multi-adaptor scaffolding membrane...

... study characterizes the structural requirements for human septin–septin interactions using a yeast two-hybrid system. We focus on three septins that are highly expressed in platelets and neurons, SEPT4 [previously designated H5, CDCrel-2 (cell-division-control-related-2), PNUTL2], SEPT5 (CDCrel-1...

...Junji HIROTA; Hideaki ANDO; Kozo HAMADA; Katsuhiko MIKOSHIBA The inositol 1,4,5-trisphosphate (IP 3 ) receptor (IP 3 R) is an intracellular IP 3 -gated Ca 2+ channel that is located on intracellular Ca 2+ stores and modulates Ca 2+ signalling. Using the yeast two-hybrid system, we screened a mouse...

..., on the basis of similar experiments, ARF6 (Class III) may be involved in recruitment of GGA proteins to other compartments, possibly early endosomes. Key words: c-adaptin, brefeldin A, trans-Golgi network, yeast two-hybrid system. c- and b1-adaptins, l1 and r1 in AP-1; a- and b2-adaptins, l2 and r2 in AP-2; d...

...-lipoxygenase protein binding yeast two-hybrid system Abbreviations used: ABP1, actin-binding protein 1; CLP, coactosin-like protein; EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide hydrochloride; F-actin, filamentous actin; G-actin, globular actin; GST, glutathione S-transferase; HA...

... proteins that might interact with the large cytoplasmic loop of Na,K-ATPase and potentially affect the function and/or the disposition of the pump in the cell, the yeast two-hybrid system was used to screen a rat skeletal muscle cDNA library. Several cDNA clones were isolated, some of which coded...

...Tadao HASEGAWA; Hengyi XIAO; Fumiyasu HAMAJIMA; Ken-ichi ISOBE GADD34 is one of a subset of proteins induced after DNA damage or cell growth arrest. To examine the function of GADD34, we used the yeast two-hybrid system to clone the protein that interacts with murine GADD34. As bait we used...

...Tadao HASEGAWA; Akihide TAKEUCHI; Osamu MIYAISHI; Hengyi XIAO; Jalin MAO; Ken-ichi ISOBE We have used the yeast two-hybrid system to clone the protein that interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor that was cloned previously...

... and 1162 are required for this interaction. 1 To whom correspondence should be addressed (e-mail [email protected]). 6 4 1999 27 4 1999 27 5 1999 The Biochemical Society, London © 1999 1999 tyrosine kinase tyrosine phosphorylation yeast two-hybrid system...