.... GST pull-down experiments and molecular docking studies revealed that the N-terminal zinc finger domain of PRMT3 binds to the C-terminal zinc finger regions of ZNF200. Furthermore, the evolutionary conservation of the Znf domain of PRMT3 correlates with the emergence of ZNF200 in mammals. We found...

... of telomere maintenance recruit NuRD to their telomeres. This interaction is mediated by the N-terminal domain of the zinc-finger protein ZNF827. NuRD–ZNF827 plays a vital role in the ALT pathway by creating a molecular platform for recombination-mediated repair. Disruption of NuRD binding results in loss...

...Eneda Toska; Stefan G. E. Roberts The WT1 (Wilms’ tumour 1) gene encodes a zinc finger transcription factor and RNA-binding protein that direct the development of several organs and tissues. WT1 manifests both tumour suppressor and oncogenic activities, but the reasons behind these opposing...

... DHHC domain suggests that it could fold as a zinc-finger domain, co-ordinating two zinc atoms in a CCHC arrangement. All residues predicted to be involved in the co-ordination of zinc were found to be essential for Swf1 function in the screen. Moreover, these mutations result in unstable proteins...

... 10 5 2013 3 6 2013 3 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 activation loop hydrophobic motif mammalian target of rapamycin (mTOR) signalling phosphorylation ribosomal protein subunit 6 kinase 1 (S6K1) zinc finger The full-length...

... interact with Jun proteins are largely unknown. © The Authors Journal compilation © 2012 Biochemical Society 2012 c-Jun MEKK1 protein kinase transcription factor ubiquitylation zinc finger Total protein (500 μg) from transfected HEK-293T cell lysates was brought to a volume of 350 μl...

... the 1.9 Å (1 Å=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domains, a CCCH-type ZnF (zinc finger) and a RRM (RNA recognition motif). Unexpectedly, the ZnF domain and the RRM form a single folding unit, glued...

...-L1Tc recombinant protein has at least a 16-fold higher affinity for single-stranded than for double-stranded nucleic acids, and that it exhibits a clear preference for RNA binding over DNA. The C2-L1Tc binding profile (to RNA and DNA) corresponds to a non-co-operative-binding model. The zinc fingers...

... recognized through the identification of three closely related proteins: RNF114, RNF138 and RNF166. TRAC-1 and its relatives were found to contain, apart from the RING domain, a C2HC (Cys 2 -His-Cys)- and two C2H2 (Cys 2 -His 2 )-type zinc fingers, as well as a UIM (ubiquitin-interacting motif). The UIM...

...Shih-Ming Huang; Sheng-Ping Huang; Sung-Ling Wang; Pei-Yao Liu Zac1, a novel seven-zinc-finger transcription factor, preferentially binds GC-rich DNA elements and has intrinsic transactivation activity. To date, the NLS (nuclear localization signal) of Zac1 has not been empirically determined. We...

...Rafal SWIERCZ; Maria D. PERSON; Mark T. BEDFORD PRMT3 (protein arginine methyltransferase 3) is one of four type I arginine methyltransferases that catalyse the formation of asymmetric dimethylarginine. PRMT3 is unique in that its N-terminus harbours a C 2 H 2 zinc-finger domain that is proposed...

... of a sizeable number of published studies and must be considered in future experiments. CREB-binding protein (CBP) p300 chelation denaturation EDTA transcription factor zinc-binding domain zinc finger The effects of Zn 2+ and EDTA on the C/H1–MDM2 interaction indicated by Matt et al. [ 5...

... double minute protein (MDM2) protein secondary structure zinc finger CBP [CREB (cAMP-response-element-binding protein)-binding protein]/p300 are members of a family of transcriptional co-activators which are involved in cell growth, development, and differentiation [ 1 – 4 ]. Most...

...Robert BABB; Benjamin R. BOWEN Peroxisome-proliferator-activated receptors (PPARs), members of the nuclear hormone receptor superfamily, play an important role in the regulation of lipid metabolism and energy homoeostasis. In a yeast two-hybrid experiment using the zinc-finger transcription factor...

... 2 1999 29 3 1999 6 4 1999 The Biochemical Society, London © 1999 1999 inositol 1,3,4,5-tetrakisphosphate pleckstrin homology domain zinc finger Biochem. J. (1999) 340, 359 363 (Printed in Great Britain) 359 RESEARCH COMMUNICATION Identification of centaurin-a1...