Regardless of whether apoptosis or necrosis are elicited by toxicants or by pathophysiological conditions, they are considered conceptually distinct forms of cell death. Nevertheless, there is increasing evidence that classical apoptosis and necrosis represent only the extreme ends of a wide range of possible morphological and biochemical deaths. The two classical types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus and, often, the intensity of the same initial insult decides the prevalence of either apoptosis or necrosis. The execution of the death programme seems to involve a relatively limited number of pathways. In many instances, their ordered execution results in characteristic morphological and biochemical changes termed apoptosis. However, some subroutines of the degradation programme may not be active in all cases of cell death. Then, the morphological appearance of dying cells and some of their biochemical alterations differ from those of classical apoptosis. We have recently shown that intracellular energy levels and mitochondrial function are rapidly compromised in necrosis, but not in apoptosis, of neuronal cells. Then we went on to show that pre-emptying human T-cells of ATP switches the type of demise caused by two classic apoptotic triggers (staurosporine and CD95 stimulation) from apoptosis to necrosis. Conditions of controlled intracellular ATP depletion, obtained by blocking mitochondrial and/or glycolytic ATP generation, were used in combination with repletion of the cytosolic ATP pool with glucose to redirect the death programme towards apoptosis or necrosis.
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September 1999
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September 01 1999
Apoptosis and necrosis: different execution of the same death
Pierluigi Nicotera;
Pierluigi Nicotera
1
1Faculty of Biology, University of Konstanz, PO Box 5560 X911, D-78434 Konstanz, Germany
1To whom correspondence should be addressed.
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Marcel Leist;
Marcel Leist
1Faculty of Biology, University of Konstanz, PO Box 5560 X911, D-78434 Konstanz, Germany
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Elisa Ferrando-May
Elisa Ferrando-May
1Faculty of Biology, University of Konstanz, PO Box 5560 X911, D-78434 Konstanz, Germany
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Publisher: Portland Press Ltd
Online ISSN: 1744-1439
Print ISSN: 0067-8694
© 1999 The Biochemical Society
1999
Biochem Soc Symp (1999) 66: 69–73.
Citation
Guy C. Brown, David G. Nicholls, Chris E. Cooper, Pierluigi Nicotera, Marcel Leist, Elisa Ferrando-May; Apoptosis and necrosis: different execution of the same death. Biochem Soc Symp 1 September 1999; 66 69–73. doi: https://doi.org/10.1042/bss0660069
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