Glycosylation is one of the most common post-translational protein modifications. Carbohydrate-mediated interactions between cells and their environment are important in differentiation, embryogenesis, inflammation, cancer and metastasis and other processes. Humans and mice with mutations that prevent normal N-glycosylation show multi-systemic defects in embryogenesis, thereby proving that these molecules are essential for normal development; however, a large number of proteins undergo defective glycosylation in these human and mouse mutants, and it is therefore difficult to determine the precise molecular roles of specific N-glycans on individual proteins. We describe here a 'functional post-translational proteomics' approach that is designed to determine the role of N-glycans on individual glycoproteins in the development of Caenorhabditis elegans.
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Conference Article|
October 01 2002
Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans
Harry Schachter;
Harry Schachter
1
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
†Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
1To whom correspondence should be addressed at Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8 (e-mail [email protected]).
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Shihao Chen;
Shihao Chen
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
‡Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, U.S.A.
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Wenli Zhang;
Wenli Zhang
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
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Andrew M. Spence;
Andrew M. Spence
§Department of Molecular and Medical Genetics, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
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Shaoxian Zhu;
Shaoxian Zhu
§Department of Molecular and Medical Genetics, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
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John W. Callahan;
John W. Callahan
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
†Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
∥Department of Paediatrics, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
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Don J. Mahuran;
Don J. Mahuran
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
¶Department of Medicine, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
**Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S 1A8
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Xiaolian Fan;
Xiaolian Fan
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
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Rick D. Bagshaw;
Rick D. Bagshaw
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
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Yi-Min She;
Yi-Min She
*Department of Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
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J Cesar Rosa;
J Cesar Rosa
††Department of Chemistry, University of New Hampshire, Durham, NH 03824, U.S.A.
‡‡Protein Chemistry Center, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto/SP, Brazil
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Vernon N. Reinhold
Vernon N. Reinhold
††Department of Chemistry, University of New Hampshire, Durham, NH 03824, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1744-1439
Print ISSN: 0067-8694
© 2002 The Biochemical Society
2002
Biochem Soc Symp (2002) 69: 1–21.
Citation
Kurt Drickamer, Anne Dell, Harry Schachter, Shihao Chen, Wenli Zhang, Andrew M. Spence, Shaoxian Zhu, John W. Callahan, Don J. Mahuran, Xiaolian Fan, Rick D. Bagshaw, Yi-Min She, J Cesar Rosa, Vernon N. Reinhold; Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans. Biochem Soc Symp 1 October 2002; 69 1–21. doi: https://doi.org/10.1042/bss0690001
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