The implementation of highly sensitive and rapid mass spectrometric screening strategies for defining the glycosylation repertoires of organs in knockout mice is helping to reveal the roles that glycans play in health and disease. Thus novel glycosylation pathways have been uncovered in two such knockouts, namely α-mannosidase II null mice and UDP-N-acetylglucosamine:α6-d-mannosideϐ1,2-N-acetylglucosaminyltransferase II null mice. This chapter documents the glycosylation profiles of a wide range of organs from the normal mouse which should facilitate future glycomics studies of knockout mice. Furthermore, we report applications of our screening technology in studies of the myodystrophy mouse and a human leukodystrophy.
MS screening strategies: investigating the glycomes of knockout and myodystrophic mice and leukodystrophic human brains
- Views Icon Views
- Share Icon Share
Kurt Drickamer, Anne Dell, Mark Sutton-Smith, Howard R. Morris, Prabhjit K. Grewal, Jane E. Hewitt, Reginald E. Bittner, Ehud Goldin, Raphael Schiffmann, Anne Dell; MS screening strategies: investigating the glycomes of knockout and myodystrophic mice and leukodystrophic human brains. Biochem Soc Symp 1 October 2002; 69 105–115. doi: https://doi.org/10.1042/bss0690105
Download citation file: