The archaeal basal transcriptional machinery consists of TBP (TATA-binding protein), TFB (transcription factor B; a homologue of eukaryotic TFIIB) and an RNA polymerase that is structurally very similar to eukaryotic RNA polymerase II. This constellation of factors is sufficient to assemble specifically on a TATA box-containing promoter and to initiate transcription at a specific start site. We have used this system to study the functional interaction between basal transcription factors and RNA polymerase, with special emphasis on the post-recruitment function of TFB. A bioinformatics analysis of the B-finger of archaeal TFB and eukaryotic TFIIB reveals that this structure undergoes rapid and apparently systematic evolution in archaeal and eukaryotic evolutionary domains. We provide a detailed analysis of these changes and discuss their possible functional implications.
Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB
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Stefan G.E. Roberts, Robert O.J. Weinzierl, Robert J. White, Finn Werner, Simone Wiesler, Sven Nottebaum, Robert O.J. Weinzierl; Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB. Biochem Soc Symp 1 January 2006; 73 49–58. doi: https://doi.org/10.1042/bss0730049
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