There is evidence that one critically short telomere may be recognized as DNA damage and, as a consequence, induce a p53/p21WAF- and pl6INK4A-dependent G1 cell cycle checkpoint to cause senescence. Additionally, senescence via a p53-and pl6INK4A-dependent mechanism can be induced by the over- or under-stimulation of certain signalling pathways that are involved in cancer. Central to this alternative senescence mechanism is the pl4ARF protein, which connects oncogene activation, but not DNA damage, to p53 activation and senescence. We find that immortal keratinocytes almost invariably have dysfunctional p53 and p16 and have high levels of telomerase, but very often express a wild-type pl4ARF. Furthermore, when normal keratinocytes senesce they show a striking elevation of p16 protein, but not of p14ARF or its downstream targets p53 and p21WAF. These results suggest that p16, rather than pl4ARF, is the more important gene in human keratinocyte senescence, but do not exclude a co-operative role for pl4ARF, perhaps in the induction of senescence by activated oncogenes in neoplasia. Regardless of mechanism, these results suggest that replicative senescence acts as a barrier to human cancer development.
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Conference Article|
February 01 2000
Replicative senescence as a barrier to human cancer Available to Purchase
E. K. Parkinson;
E. K. Parkinson
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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J. Munro;
J. Munro
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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K. Steeghs;
K. Steeghs
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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V. Morrison;
V. Morrison
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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H. Ireland;
H. Ireland
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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N. Forsyth;
N. Forsyth
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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S. Fitzsimmons;
S. Fitzsimmons
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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S. Bryce
S. Bryce
1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, U.K.
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Publisher: Portland Press Ltd
Received:
August 05 1999
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (2): 226–233.
Article history
Received:
August 05 1999
Citation
E. K. Parkinson, J. Munro, K. Steeghs, V. Morrison, H. Ireland, N. Forsyth, S. Fitzsimmons, S. Bryce; Replicative senescence as a barrier to human cancer. Biochem Soc Trans 1 February 2000; 28 (2): 226–233. doi: https://doi.org/10.1042/bst0280226
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