Growth and differentiation factor-5 (GDF-5) is a divergent member of the transforming growth factor-β/bone morphogenetic protein (BMP) superfamily that is required for proper skeletal patterning and development in the vertebrate limb. Based on the homology of GDF-5 with other bone-inducing BMP family members, the inductive activity of a recombinant form of human GDF-5 (rhGDF-5) was evaluated in a series of in vitro assays and in vivo bone-formation models. The in vitro response to rhGDF-5 resulted in the formation of chondrogenic nodules in fetal rat calvarial cells cultured in the context of collagen or collagen/hyaluronate extracellular matrices. Matrices loaded with rhGDF-5 induced ectopic cartilaginous and osseous tissue when implanted in subcutaneous or intramuscular sites. In non-human primate long-bone-defect and spinal-fusion models, rhGDF-5 combined with a mineralized collagen matrix induced bone formation in a manner equivalent to autogenous bone. These results highlight the unique potential of rhGDF-5 in a wide variety of orthopaedic applications.
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Conference Article|
August 01 2000
Inductive activity of recombinant human growth and differentiation factor-5
R. C. Spiro;
R. C. Spiro
1
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
1To whom correspondence should be addressed (e-mail bspiro@orquest.com)
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L.-S. Liu;
L.-S. Liu
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
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M. A. Heidaran;
M. A. Heidaran
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
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A. Y. Thompson;
A. Y. Thompson
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
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C. K. Ng;
C. K. Ng
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
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J. Pohl;
J. Pohl
†Biopharm GmbH, Czemyring 22, 69115 Heidelberg, Germany
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J. W. Poser
J. W. Poser
*Orquest, Inc., 365 Ravendale Drive, Mountain View, CA 94043, U.S.A.
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Publisher: Portland Press Ltd
Received:
March 01 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (4): 362–368.
Article history
Received:
March 01 2000
Citation
R. C. Spiro, L.-S. Liu, M. A. Heidaran, A. Y. Thompson, C. K. Ng, J. Pohl, J. W. Poser; Inductive activity of recombinant human growth and differentiation factor-5. Biochem Soc Trans 1 August 2000; 28 (4): 362–368. doi: https://doi.org/10.1042/bst0280362
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