Fatty acids are accumulated in triacylglycerols (TAGs), in specialized organelles of seeds named oil bodies. The major site of TAG accumulation is detected in developing seed and mesocarp of certain species. We have isolated two cDNAs encoding DGAT enzymes from olives. The deduced polypeptides differ by 26 amino acids in size. However, they have high homology and almost identical hydropathy profiles. The DGAT gene is expressed in all tissues that synthesize TAGs. However, higher levels of DGAT transcripts have been detected in seed tissues of developing olive drupe. DGAT expression and mRNA accumulation in drupe tissues is developmentally regulated. Each DGAT transcript shows a distinct profile of accumulation. The existence of two different DGAT transcripts might reflect two different enzymes with discrete function and/or localization.
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Conference Article|
December 01 2000
Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues
K. Giannoulia;
K. Giannoulia
1Laboratory of Molecular Biology, Agricultural University of Athens, lera Odos 75, 118 55 Athens, Greece
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K. Haralampidis;
K. Haralampidis
1Laboratory of Molecular Biology, Agricultural University of Athens, lera Odos 75, 118 55 Athens, Greece
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Z. Poghosyan;
Z. Poghosyan
1Laboratory of Molecular Biology, Agricultural University of Athens, lera Odos 75, 118 55 Athens, Greece
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D. J. Murphy;
D. J. Murphy
1Laboratory of Molecular Biology, Agricultural University of Athens, lera Odos 75, 118 55 Athens, Greece
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P. Hatzopoulos
P. Hatzopoulos
1
1Laboratory of Molecular Biology, Agricultural University of Athens, lera Odos 75, 118 55 Athens, Greece
1To whom correspondence should be addressed (e-mail [email protected])
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Publisher: Portland Press Ltd
Received:
June 23 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 695–697.
Article history
Received:
June 23 2000
Citation
K. Giannoulia, K. Haralampidis, Z. Poghosyan, D. J. Murphy, P. Hatzopoulos; Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues. Biochem Soc Trans 1 December 2000; 28 (6): 695–697. doi: https://doi.org/10.1042/bst0280695
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