Inverted-repeat-based gene constructs targeted against two key cotton seed-specific fatty acid desaturase genes, ghSAD-1 encoding stearoylacyl carrier protein Δ9-desaturase and ghFAD2-1 encoding microsomal ω-6 desaturase, were transformed into cotton. The expression of ghSAD-1 and ghFAD2-1 in the inverted-repeat orientation resulted in increased levels of stearic and oleic acids, respectively. Interestingly, the content of palmitic acid in both high-stearic and high-oleic lines was substantially reduced. These materials offer the promise of developing cotton seed oil products with greatly improved nutritional appeal to consumers.
Conference Article| December 01 2000
Genetic modification of cotton seed oil using inverted-repeat gene-silencing techniques
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Q. Liu, S. Singh, A. Green; Genetic modification of cotton seed oil using inverted-repeat gene-silencing techniques. Biochem Soc Trans 1 December 2000; 28 (6): 927–929. doi: https://doi.org/10.1042/bst0280927
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