We have generated antisense plants of Brassica napus, targeting either the cytoplasmic Type I acetyl-CoA carboxylase or the β-ketoacyl-acyl carrier protein reductase of the Type II dissociable fatty acid synthase, in order to investigate the importance of these components in regulating lipid metabolism in plants. Using a range of down-regulated plants, it has become clear that down-regulation of these genes also causes down-regulation of other components of lipid metabolism, at the activity, translational and transcriptional levels. These plants exhibit effects, not immediately predicted, on oil yield and carbon resource allocation in seeds.
Investigations into the regulation of lipid biosynthesis in Brassica napus using antisense down-regulation
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A. R. Slabas, A. White, P. O'Hara, T. Fawcett; Investigations into the regulation of lipid biosynthesis in Brassica napus using antisense down-regulation. Biochem Soc Trans 1 November 2002; 30 (6): 1056–1058. doi: https://doi.org/10.1042/bst0301056
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