The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2′,3′-cyclic and 5′-hydroxy termini. The cleavage rate is approx. 1 min-1 at 25 °C, pH 7.5, and increases proportionally with hydroxide ion concentration between pH 5 and pH 9. As the rate of non-catalysed cleavage of RNA to form the same products is approx. 10-6 min-1 under similar conditions, an important goal is to understand how the folded structure of this small ribozyme increases the rate of alkaline cleavage 106-fold at this specific site.
Conference Article| November 01 2002
The hammerhead ribozyme
K. F. Blount;
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K. F. Blount, O. C. Uhlenbeck; The hammerhead ribozyme. Biochem Soc Trans 1 November 2002; 30 (6): 1119–1122. doi: https://doi.org/10.1042/bst0301119
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