Interactions between an immobilized, heparin-derived octasaccharide and growth factors have been observed using a quartz crystal microbalance-dissipation (QCM-D). This device can measure the amount of growth factors binding to the octasaccharide surface and also the change of dissipation of the surface. Dissipation is a measure of how the adhered material ‘damps’ the surface vibrations. The octasaccharides were anchored through their reducing ends by the intermediary of the alkanethiol molecule, which covalently binds to the crystal surface through the thiol group. As expected, heparin sulphate binding growth factors bound to the octasaccharide, but the change in mass of growth factor bound per unit change in dissipation is different for the different growth factors. Suggesting that the structures of the various growth factor–octasaccharide complexes are different, therefore, indicates that the change in dissipation can give insights into the structure, orientation and packing of the oligosaccharide-growth factor complexes.
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April 2003
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Conference Article|
April 01 2003
A gravimetric analysis of protein–oligosaccharide interactions
T. Rudd
;
T. Rudd
*School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K.
†Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K.
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J.T. Gallagher
;
J.T. Gallagher
‡Cancer Research Campaign Department of Medical Oncology, University of Manchester, Christie Hospital National Health Service Trust, Wilmslow Road, Manchester M20 4BX, U.K.
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D. Ron
;
D. Ron
§Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
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R.J. Nichols
;
R.J. Nichols
†Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K.
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D.G. Fernig
D.G. Fernig
1
*School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K.
1To whom correspondence should be addressed (e-mail dgfernig@liv.ac.uk).
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Biochem Soc Trans (2003) 31 (2): 349–351.
Citation
T. Rudd, J.T. Gallagher, D. Ron, R.J. Nichols, D.G. Fernig; A gravimetric analysis of protein–oligosaccharide interactions. Biochem Soc Trans 1 April 2003; 31 (2): 349–351. doi: https://doi.org/10.1042/bst0310349
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