The production of ROS (reactive oxygen species) by the mitochondrial respiratory chain contributes to a range of pathologies, including neurodegenerative diseases, ischaemia/reperfusion injury and aging. There are also indications that mitochondrial ROS production plays a role in damage response and signal transduction pathways. To unravel the role of mitochondrial ROS production in these processes, we have developed a range of mitochondria-targeted probe molecules. Covalent attachment of a lipophilic cation leads to their accumulation into mitochondria, driven by the membrane potential. Molecules developed so far include antioxidants designed to intercept mitochondrial ROS and reagents that specifically label mitochondrial thiol proteins. Here we outline how mitochondrial ROS formation and its consequences can be investigated using these probes.
Skip Nav Destination
Article navigation
December 2003
- PDF Icon PDF LinkFront Matter
Conference Article|
December 01 2003
Using mitochondria-targeted molecules to study mitochondrial radical production and its consequences
R.A.J. Smith;
R.A.J. Smith
*Department of Chemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
G.F. Kelso;
G.F. Kelso
*Department of Chemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
F.H. Blaikie;
F.H. Blaikie
*Department of Chemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
C.M. Porteous;
C.M. Porteous
†Department of Biochemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
E.C. Ledgerwood;
E.C. Ledgerwood
†Department of Biochemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
G. Hughes;
G. Hughes
†Department of Biochemistry, University of Otago, Box 56, Dunedin, New Zealand
Search for other works by this author on:
A.M. James;
A.M. James
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
M.F. Ross;
M.F. Ross
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
J. Asin-Cayuela;
J. Asin-Cayuela
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
H.M. Cochemé;
H.M. Cochemé
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
A. Filipovska;
A. Filipovska
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
M.P. Murphy
M.P. Murphy
1
‡Medical Research Council–Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
1To whom correspondence should be addressed (e-mail [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2003 Biochemical Society
2003
Biochem Soc Trans (2003) 31 (6): 1295–1299.
Citation
R.A.J. Smith, G.F. Kelso, F.H. Blaikie, C.M. Porteous, E.C. Ledgerwood, G. Hughes, A.M. James, M.F. Ross, J. Asin-Cayuela, H.M. Cochemé, A. Filipovska, M.P. Murphy; Using mitochondria-targeted molecules to study mitochondrial radical production and its consequences. Biochem Soc Trans 1 December 2003; 31 (6): 1295–1299. doi: https://doi.org/10.1042/bst0311295
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.