RNase A (1 mM) was incubated with glucose (0.4 M) at 37°C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys1, Lys7, Lys37 and Lys41. Three of these sites (Lys7, Lys37 and Lys41) were also the major sites of N∊-(carboxymethyl)lysine formation.
Mass spectrometric analysis of glucose-modified ribonuclease
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W.E. Cotham, D.J.S. Hinton, T.O. Metz, J.W.C. Brock, S.R. Thorpe, J.W. Baynes, J.M. Ames; Mass spectrometric analysis of glucose-modified ribonuclease. Biochem Soc Trans 1 December 2003; 31 (6): 1426–1427. doi: https://doi.org/10.1042/bst0311426
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