Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.
Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging
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I. Karakikes, R.E. Barber, I.E.G. Morrison, N. Fernández, R.J. Cherry; Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging. Biochem Soc Trans 1 December 2003; 31 (6): 1453–1455. doi: https://doi.org/10.1042/bst0311453
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