In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus caldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments
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T.J. Rahman, R. Marchant, I.M. Banat; Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments. Biochem Soc Trans 1 April 2004; 32 (2): 209–213. doi: https://doi.org/10.1042/bst0320209
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