Neutrophil purification has traditionally been performed by centrifugation of leucocytes through density gradients. These reliable methods produce populations that are typically >95% pure neutrophils, and have allowed the widespread study of the function of these cells. Our recent work has suggested that residual monocytes may play a more important role than has been previously realized, and suggest that for some functional experiments, further purification of cells is required to understand fully the neutrophil phenotype.
Conference Article| June 01 2004
What can we learn from highly purified neutrophils?
Biochem Soc Trans (2004) 32 (3): 468-469.
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I. Sabroe, L.R. Prince, S.K. Dower, S.R. Walmsley, E.R Chilvers, M.K.B. Whyte; What can we learn from highly purified neutrophils?. Biochem Soc Trans 1 June 2004; 32 (3): 468–469. doi: https://doi.org/10.1042/bst0320468
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