Wnt signalling in development operates via members of the Frizzleds, G-protein-coupled receptors that bind specific Wnt ligands and mediate signalling via distinct pathways. The Wnt/Ca2+/cGMP pathway mediated by Frizzled-2 was discovered recently. Activation of this pathway leads to increased intracellular concentrations of Ca2+ and decreased intracellular concentrations of cGMP. The nature of the phosphodiesterase responsible for this Frizzled-2-mediated effect on cGMP levels was identified based on three separate criteria: (i) sensitivity to selective enzyme inhibitors, (ii) behaviour on chromatographic separation, and (ii) isolation by two-dimensional gels in tandem with direct mapping by MS of tryptic digests of the activity. On the basis of results from these three analyses, the cGMP-specific phosphodiesterase, PDE6, is demonstrated to be an effector for the Wnt/Ca2+/cGMP signalling pathway of development, which is mediated by Frizzled-2.
Conference Article| October 26 2004
PDE6 is an effector for the Wnt/Ca2+/cGMP-signalling pathway in development
H. Wang 1
*Department of Physiology and Biophysics, Health Sciences Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8661, U.S.A.
1To whom correspondence should be addressed (email email@example.com).
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H. Wang, Y. Lee, C.C. Malbon; PDE6 is an effector for the Wnt/Ca2+/cGMP-signalling pathway in development. Biochem Soc Trans 1 November 2004; 32 (5): 792–796. doi: https://doi.org/10.1042/BST0320792
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