A substantial number of G-protein-coupled receptor-interacting proteins have been identified initially by the use of yeast two-hybrid screens. Using the C-terminal tail of both opioid receptors and the melanin concentrating hormone receptor-1 as bait, the actin and intermediate filament-binding protein periplakin was isolated. In each case, the site of interaction is within helix VIII of the receptor and periplakin limits agonist-mediated G-protein activation potentially by competing with G-protein for this region of the receptor.
Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation
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G. Milligan, H. Murdoch, E. Kellett, J.H. White, G.-J. Feng; Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation. Biochem Soc Trans 1 November 2004; 32 (5): 878–880. doi: https://doi.org/10.1042/BST0320878
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