CPPs (cell-penetrating peptides) facilitate cellular uptake of covalently attached macromolecules, through an as yet controversial mechanism that either involves direct membrane passage or a type of endocytosis. We investigated the potential of the CPPs penetratin and Tat to act as mitochondria-targeting vectors by testing whether they were internalized by isolated mitochondria, and by mitochondria within cells in culture. We also tested peptides conjugated to the mitochondria-targeting moiety triphenylphosphonium. We found no evidence for mitochondrial uptake by penetratin, Tat or their triphenylphosphonium conjugates. This result suggests that CPPs are unsuitable as mitochondria-targeting vectors, and implies an endocytic mode of cellular uptake for CPPs.
Conference Article| October 26 2004
Cell-penetrating peptides are excluded from the mitochondrial matrix
- Views Icon Views
- Share Icon Share
- Cite Icon Cite
M.F. Ross, M.P. Murphy; Cell-penetrating peptides are excluded from the mitochondrial matrix. Biochem Soc Trans 1 November 2004; 32 (6): 1072–1074. doi: https://doi.org/10.1042/BST0321072
Download citation file: