The structure–function relationship in group V of C-type animal lectins remains incompletely understood despite the new structures of NK (natural killer) cell receptors that have been solved recently. Recombinant, soluble forms of rat and human NKR-P1 and CD69 that we obtained after in vitro refolding were analysed by Fourier transform–ion cyclotron resonance MS and heteronuclear NMR (1H-15N correlation). In NKR-P1, calcium may not be removed by chelating agents because of the very high affinity of binding. In CD69, incorporation of calcium causes a structural shift in several amino acids important for the interaction with carbohydrates. Structural studies have also allowed us to understand an interesting preference of these receptors for either linear (NKR-P1) or branched (CD69) carbohydrate sequences.
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November 2004
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Conference Article|
October 26 2004
Lymphocyte activation receptors: new structural paradigms in group V of C-type animal lectins
J. Pavlíček;
J. Pavlíček
1
*Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
1Correspondence should be addressed at Hlavova 8, CZ-12840 Praha 2, Czech Republic (email pavlicek@biomed.cas.cz).
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D. Kavan;
D. Kavan
*Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
†Institute of Microbiology, Academy of Sciences of Czech Republic, Praha, Czech Republic
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P. Pompach;
P. Pompach
*Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
†Institute of Microbiology, Academy of Sciences of Czech Republic, Praha, Czech Republic
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P. Novák;
P. Novák
†Institute of Microbiology, Academy of Sciences of Czech Republic, Praha, Czech Republic
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O. Lukšan;
O. Lukšan
*Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
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K. Bezouška
K. Bezouška
*Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
†Institute of Microbiology, Academy of Sciences of Czech Republic, Praha, Czech Republic
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Publisher: Portland Press Ltd
Received:
July 02 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (6): 1124–1126.
Article history
Received:
July 02 2004
Citation
J. Pavlíček, D. Kavan, P. Pompach, P. Novák, O. Lukšan, K. Bezouška; Lymphocyte activation receptors: new structural paradigms in group V of C-type animal lectins. Biochem Soc Trans 1 November 2004; 32 (6): 1124–1126. doi: https://doi.org/10.1042/BST0321124
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