Rhizobium etli CFN42 is not capable of growing anaerobically with nitrate but it grows with nitrite as a terminal electron acceptor. This bacterium contains the nirK gene encoding the copper-containing Nir (nitrite reductase), which is located on the cryptic plasmid pCFN42f. Mutational analysis has demonstrated that a nirK deficient mutant was not capable of growing under nitrite-respiring conditions. Moreover, microaerobic growth of this mutant was inhibited by the presence of nitrite. Nir activity and nitrite uptake were highly diminished in a nirK mutant, compared with the wild-type levels after incubation under anaerobic conditions. Our results suggest that the copper-containing Nir may have both a respiratory and a nitrite-detoxifying role in R. etli.
Function of the Rhizobium etli CFN42 nirK gene in nitrite metabolism
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E. Bueno, N. Gómez-Hernández, L. Girard, E.J. Bedmar, M.J. Delgado; Function of the Rhizobium etli CFN42 nirK gene in nitrite metabolism. Biochem Soc Trans 1 February 2005; 33 (1): 162–163. doi: https://doi.org/10.1042/BST0330162
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