Phospholamban (PLB) is a 52 amino acid transmembrane protein found in the sarcoplasmic reticulum of cardiac myocytes, where it regulates the transport of calcium ions by SERCA (sarcoplasmic/endoplasmic reticulum Ca2+-ATPase). This work has shown that the cytoplasmic domain of PLB associates with phospholipid vesicles, possibly with the lipid polar head groups, and, in doing so, undergoes a transition from a random coil to a more helical conformation. These findings support a recent hypothesis that the cytoplasmic domain of PLB acts as a conformational switch, alternating between an orientation that lies across the membrane surface and an upright orientation that associates with the regulatory site of SERCA.
Conference Article| October 26 2005
Spectroscopic studies of phospholamban variants in phospholipid bilayers
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J.C. Clayton, E. Hughes, D.A. Middleton; Spectroscopic studies of phospholamban variants in phospholipid bilayers. Biochem Soc Trans 26 October 2005; 33 (5): 913–915. doi: https://doi.org/10.1042/BST0330913
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