Polarized transport of the plant hormone auxin influences multiple growth processes in plants and is regulated by plasma-membrane-localized efflux and uptake carriers. The PGP (P-glycoprotein) ABC transporters (ATP-binding-cassette transporters), PIN (pin-formed) subfamily of major facilitator proteins and members of AUX/LAX families have been shown to independently transport auxin both in planta and in heterologous systems. However, PIN- and PGP-mediated transport in heterologous systems exhibits decreased substrate specificity and inhibitor-sensitivity compared with what is seen in plants and plant cells. To determine whether PIN–PGP interactions enhance transport specificity, we analysed interactions of the representative auxin-transporting PGPs with PIN1 and AUX1 in planta and in heterologous systems. Here, we provide evidence that PINs and PGPs interact and function both independently and co-ordinately to control polar auxin transport and impart transport specificity and directionality. These interactions take place in protein complexes stabilized by PGPs in detergent-resistant microdomains.
Interactions of PIN and PGP auxin transport mechanisms
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A. Bandyopadhyay, J.J. Blakeslee, O.R. Lee, J. Mravec, M. Sauer, B. Titapiwatanakun, S.N. Makam, R. Bouchard, M. Geisler, E. Martinoia, J. Friml, W.A. Peer, A.S. Murphy; Interactions of PIN and PGP auxin transport mechanisms. Biochem Soc Trans 1 February 2007; 35 (1): 137–141. doi: https://doi.org/10.1042/BST0350137
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