This contribution describes how de novo designed synthetic helix–loop–helix polypeptides are utilized to control the assembly of gold nanoparticles and as scaffolds for biosensing. The synthetic polypeptides are designed to fold into a four-helix bundle upon dimerization. When immobilized on gold nanoparticles, dimerization and folding occur between peptides on neighbouring particles as an effect of particle aggregation and the folded polypeptides are rigid enough to keep the particles separated at a distance corresponding to the size of the four-helix bundle. Moreover, peptide dimerization offers a convenient route to assemble nanoparticles into hybrid multilayers on planar substrates. The drastic change in the resonance conditions of the localized nanoparticle surface plasmon upon particle aggregation is shown to be useful for optical detection of biomolecular interactions.
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June 2007
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Conference Article|
May 22 2007
Synthetic de novo designed polypeptides for control of nanoparticle assembly and biosensing
D. Aili;
D. Aili
1
*Department of Physics, Biology and Chemistry, Linköping University, SE-581 83 Linköping, Sweden
1To whom correspondence should be addressed (email [email protected]).
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K. Enander;
K. Enander
*Department of Physics, Biology and Chemistry, Linköping University, SE-581 83 Linköping, Sweden
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L. Baltzer;
L. Baltzer
†Department of Chemistry, BMC, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden
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B. Liedberg
B. Liedberg
*Department of Physics, Biology and Chemistry, Linköping University, SE-581 83 Linköping, Sweden
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Publisher: Portland Press Ltd
Received:
January 02 2007
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2007 Biochemical Society
2007
Biochem Soc Trans (2007) 35 (3): 532–534.
Article history
Received:
January 02 2007
Citation
D. Aili, K. Enander, L. Baltzer, B. Liedberg; Synthetic de novo designed polypeptides for control of nanoparticle assembly and biosensing. Biochem Soc Trans 1 June 2007; 35 (3): 532–534. doi: https://doi.org/10.1042/BST0350532
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