Vascular endothelial cells play a pivotal role in regulating leucocyte recruitment during inflammation, and their responses can be modulated by their local environment, including cells of the tissue stroma. We have developed a model system to examine how the communication between endothelial cells and fibroblasts regulates the recruitment of leucocytes and their subsequent subendothelial fate. Here, we describe a novel co-culture filter-based flow assay and highlight the ability of synovial fibroblasts obtained from chronically inflamed tissue to promote leucocyte recruitment to otherwise ‘resting’ endothelial cells.
Modulation of endothelial responses by the stromal microenvironment: effects on leucocyte recruitment
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H.M. McGettrick, A. Filer, G.E. Rainger, C.D. Buckley, G.B. Nash; Modulation of endothelial responses by the stromal microenvironment: effects on leucocyte recruitment. Biochem Soc Trans 1 November 2007; 35 (5): 1161–1162. doi: https://doi.org/10.1042/BST0351161
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