Using the Edmonton protocol, a number of patients with Type 1 diabetes mellitus have remained insulin-independent for prolonged periods of time. In spite of this success, transplantation of islets from cadaver donors will remain a therapy for very few patients owing to a lack of donors. Thus, if cell therapy should be widely available, it will require an unlimited source of cells to serve as a ‘biological’ insulin pump. At this time, the development of β-cells from hESCs (human embryonic stem cells) has emerged as the most attractive alternative. It is envisioned that ultimate success of an in vitro approach to programme hESCs into β-cells will depend on the ability, at least to a certain degree, to sequentially reproduce the individual steps that characterizes normal β-cell ontogenesis during fetal pancreatic development, including definitive endoderm from which all gastrointestinal organs, including the pancreas, originate. In the present article, differentiation of hESCs into putative definitive endodermal cell types is reviewed.
Conference Article| May 21 2008
Definitive endoderm: a key step in coaxing human embryonic stem cells into transplantable β-cells
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Henrik Semb; Definitive endoderm: a key step in coaxing human embryonic stem cells into transplantable β-cells. Biochem Soc Trans 1 June 2008; 36 (3): 272–275. doi: https://doi.org/10.1042/BST0360272
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