Inosine residues may be introduced into long dsRNA (double-stranded RNA) molecules by the action of a family of editing enzymes, ADARs (adenosine deaminases that act on RNA). Furthermore, hyperediting of dsRNA by ADARs may result in up to 50% of adenosine residues being converted into inosine. While the effect of hyperediting has traditionally been thought to be limited to the edited dsRNA, we have recently shown that hyperedited dsRNA [I-dsRNA (inosine-containing dsRNA)] is able to down-regulate the expression of both reporter and endogenous mRNAs in cells, in trans. Down-regulation by I-dsRNA occurs both by reducing mRNA levels and by inhibiting of translation. This finding has important functional consequences for hyperediting by ADARs.
Conference Article| May 21 2008
Gene expression is reduced in trans by inosine-containing dsRNA
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A.D.J. Scadden; Gene expression is reduced in trans by inosine-containing dsRNA. Biochem Soc Trans 1 June 2008; 36 (3): 534–536. doi: https://doi.org/10.1042/BST0360534
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