Hydrodynamic techniques such as analytical ultracentrifugation can provide key information about subunit stoichiometry and interaction strengths of protein–nucleic acid interactions. Analysis is complicated by (i) the need for low concentrations in order to observe both free and bound species and (ii) thermodynamic non-ideality. With the introduction of fluorescence optics, we are able to obtain data at lower concentrations, and improved understanding of the statistical thermodynamics of macromolecular solutions has allowed non-ideality to be accurately assigned. With these developments, it is possible now to assay protein–nucleic acid interactions at concentrations typically used in molecular biology assays.
Conference Article| July 22 2008
The shock of the old: hydrodynamics for the masses
- Views Icon Views
- Share Icon Share
David J. Scott; The shock of the old: hydrodynamics for the masses. Biochem Soc Trans 1 August 2008; 36 (4): 766–770. doi: https://doi.org/10.1042/BST0360766
Download citation file: