The periplasmic nitrite reductase system from Escherichia coli and the extracellular Fe(III) reductase system from Shewanella oneidensis contain multihaem c-type cytochromes as electron carriers and terminal reductases. The position and orientation of the haem cofactors in multihaem cytochromes from different bacteria often show significant conservation despite different arrangements of the polypeptide chain. We propose that the decahaem cytochromes of the iron reductase system MtrA, MtrC and OmcA comprise pentahaem ‘modules’ similar to the electron donor protein, NrfB, from E. coli. To demonstrate this, we have isolated and characterized the N-terminal pentahaem module of MtrA by preparing a truncated form containing five covalently attached haems. UV–visible spectroscopy indicated that all five haems were low-spin, consistent with the presence of bis-His ligand co-ordination as found in full-length MtrA.
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Conference Article|
September 19 2008
The role of multihaem cytochromes in the respiration of nitrite in Escherichia coli and Fe(III) in Shewanella oneidensis
Thomas A. Clarke;
Thomas A. Clarke
1
*Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
1To whom correspondence should be addressed (email [email protected]).
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Tracey Holley;
Tracey Holley
*Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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Robert S. Hartshorne;
Robert S. Hartshorne
*Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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Jim K. Fredrickson;
Jim K. Fredrickson
†Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, U.S.A.
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John M. Zachara;
John M. Zachara
†Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, U.S.A.
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Liang Shi;
Liang Shi
†Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, U.S.A.
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David J. Richardson
David J. Richardson
*Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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Publisher: Portland Press Ltd
Received:
May 03 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (5): 1005–1010.
Article history
Received:
May 03 2008
Citation
Thomas A. Clarke, Tracey Holley, Robert S. Hartshorne, Jim K. Fredrickson, John M. Zachara, Liang Shi, David J. Richardson; The role of multihaem cytochromes in the respiration of nitrite in Escherichia coli and Fe(III) in Shewanella oneidensis. Biochem Soc Trans 1 October 2008; 36 (5): 1005–1010. doi: https://doi.org/10.1042/BST0361005
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