Rhodobacter sphaeroides produces a novel cytochrome, designated as SHP (sphaeroides haem protein), that is unusual in having asparagine as a redox-labile haem ligand. The gene encoding SHP is contained within an operon that also encodes a DHC (dihaem cytochrome c) and a membrane-associated cytochrome b. DHC and SHP have been shown to have high affinity for each other at low ionic strength (Kd=0.2 μM), and DHC is able to reduce SHP very rapidly. The reduced form of the protein, SHP2+ (reduced or ferrous SHP), has high affinity for both oxygen and nitric oxide (NO). It has been shown that the oxyferrous form, SHP2+–O2 (oxygen-bound form of SHP), reacts rapidly with NO to produce nitrate, whereas SHP2+–NO (the NO-bound form of SHP) will react with superoxide with the same product formed. It is therefore possible that SHP functions physiologically as a nitric oxide dioxygenase, protecting the organism against NO poisoning, and we propose a possible mechanism for this process.
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October 2008
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Conference Article|
September 19 2008
Rhodobacter sphaeroides haem protein: a novel cytochrome with nitric oxide dioxygenase activity
Bor-Ran Li;
Bor-Ran Li
*EaStCHEM, School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, U.K.
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J.L. Ross Anderson;
J.L. Ross Anderson
*EaStCHEM, School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, U.K.
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Christopher G. Mowat;
Christopher G. Mowat
*EaStCHEM, School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, U.K.
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Caroline S. Miles;
Caroline S. Miles
†Institute of Structural and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, U.K.
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Graeme A. Reid;
Graeme A. Reid
†Institute of Structural and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, U.K.
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Stephen K. Chapman
Stephen K. Chapman
1
*EaStCHEM, School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, U.K.
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
June 05 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (5): 992–995.
Article history
Received:
June 05 2008
Citation
Bor-Ran Li, J.L. Ross Anderson, Christopher G. Mowat, Caroline S. Miles, Graeme A. Reid, Stephen K. Chapman; Rhodobacter sphaeroides haem protein: a novel cytochrome with nitric oxide dioxygenase activity. Biochem Soc Trans 1 October 2008; 36 (5): 992–995. doi: https://doi.org/10.1042/BST0360992
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